目的观察肾小球足突细胞膜黏蛋白(podoplanin,PNPD)在结直肠上皮内瘤变内镜手术组织中的表达,研究其在结直肠上皮内瘤变中的作用。方法回顾性研究40例结直肠上皮内瘤变患者,采用免疫组织化学法检测内镜手术组织中肾小球足突细胞膜黏蛋...目的观察肾小球足突细胞膜黏蛋白(podoplanin,PNPD)在结直肠上皮内瘤变内镜手术组织中的表达,研究其在结直肠上皮内瘤变中的作用。方法回顾性研究40例结直肠上皮内瘤变患者,采用免疫组织化学法检测内镜手术组织中肾小球足突细胞膜黏蛋白的表达,并与40例直、结肠癌组织和40例正常组织进行对照。采用免疫组织化学法检测40例结直肠上皮内瘤变内镜手术组织、40例直、结肠癌组织和40例正常对照组织中肾小球足突细胞膜黏蛋白的表达,分析其与上皮内瘤变的关系。结果肾小球足突细胞膜黏蛋白主要表达在细胞淋巴管内皮细胞上,肾小球足突细胞膜黏蛋白标记的淋巴管密度(lymphatic vessel density of glomerular podocyte membrane mucin,LMVD)在直、结肠癌组织中表达高于上皮内瘤变组织,差异显著(P<0.05);其在上皮内瘤变组织中的表达与正常大肠组织中表达相比差异显著(P<0.05)。结论肾小球足突细胞膜黏蛋白在结直肠上皮内瘤变内镜手术组织中高表达,可以作为结直肠上皮内瘤变的标记物。展开更多
AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (...AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology. METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies. RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research. CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopie method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.展开更多
AIM： To evaluate 20 adults with intussusception and to clarify the cause, clinical features, diagnosis, and management of this uncommon entity. METHODS： A retrospective review of patients aged 〉 18 years with a dia...AIM： To evaluate 20 adults with intussusception and to clarify the cause, clinical features, diagnosis, and management of this uncommon entity. METHODS： A retrospective review of patients aged 〉 18 years with a diagnosis of intestinal intussusception between 2000 and 2008. Patients with rectal prolapse, prolapse of or around an ostomy and gastroenterostomy intussusception were excluded. RESULTS： There were 20 rases of adult intussusception. Mean age was 47.7 years. Abdominal pain, nausea, and vomiting were the most common symptoms. The majority of intussusceptions were in the small intestine （85%）. There were three （15%） cases of colonic intussusception. Enteric intussusception consisted of five jejunojejunal cases, nine ileoileal, and four cases of ileocecal invagination. Among enteric intussusceptions, 14 were secondary to a benign process, and in one of these, the malignant cause was secondary to metastatic lung adenocarcinoma. All colonic lesions were malignant. All cases were treated surgically. CONCLUSION： Adult intussusception is an unusual and challenging condition and is a preoperative diagnostic problem. Treatment usually requires resection of the involved bowel segment. Reduction can be attempted in small-bowel intussusception if the segment involved is viable or malignancy is not suspected; however, a more careful approach is recommended in colonic intussusception because of a significantly higher coexistence of malignancy.展开更多
文摘目的观察肾小球足突细胞膜黏蛋白(podoplanin,PNPD)在结直肠上皮内瘤变内镜手术组织中的表达,研究其在结直肠上皮内瘤变中的作用。方法回顾性研究40例结直肠上皮内瘤变患者,采用免疫组织化学法检测内镜手术组织中肾小球足突细胞膜黏蛋白的表达,并与40例直、结肠癌组织和40例正常组织进行对照。采用免疫组织化学法检测40例结直肠上皮内瘤变内镜手术组织、40例直、结肠癌组织和40例正常对照组织中肾小球足突细胞膜黏蛋白的表达,分析其与上皮内瘤变的关系。结果肾小球足突细胞膜黏蛋白主要表达在细胞淋巴管内皮细胞上,肾小球足突细胞膜黏蛋白标记的淋巴管密度(lymphatic vessel density of glomerular podocyte membrane mucin,LMVD)在直、结肠癌组织中表达高于上皮内瘤变组织,差异显著(P<0.05);其在上皮内瘤变组织中的表达与正常大肠组织中表达相比差异显著(P<0.05)。结论肾小球足突细胞膜黏蛋白在结直肠上皮内瘤变内镜手术组织中高表达,可以作为结直肠上皮内瘤变的标记物。
基金Supported by the National Natural Science Foundation of China, No. 30371604 State Key Project of China, No. 2002CCA01900
文摘AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology. METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies. RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research. CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopie method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.
文摘AIM： To evaluate 20 adults with intussusception and to clarify the cause, clinical features, diagnosis, and management of this uncommon entity. METHODS： A retrospective review of patients aged 〉 18 years with a diagnosis of intestinal intussusception between 2000 and 2008. Patients with rectal prolapse, prolapse of or around an ostomy and gastroenterostomy intussusception were excluded. RESULTS： There were 20 rases of adult intussusception. Mean age was 47.7 years. Abdominal pain, nausea, and vomiting were the most common symptoms. The majority of intussusceptions were in the small intestine （85%）. There were three （15%） cases of colonic intussusception. Enteric intussusception consisted of five jejunojejunal cases, nine ileoileal, and four cases of ileocecal invagination. Among enteric intussusceptions, 14 were secondary to a benign process, and in one of these, the malignant cause was secondary to metastatic lung adenocarcinoma. All colonic lesions were malignant. All cases were treated surgically. CONCLUSION： Adult intussusception is an unusual and challenging condition and is a preoperative diagnostic problem. Treatment usually requires resection of the involved bowel segment. Reduction can be attempted in small-bowel intussusception if the segment involved is viable or malignancy is not suspected; however, a more careful approach is recommended in colonic intussusception because of a significantly higher coexistence of malignancy.
