目的:观察抗病毒口服液(Antivirus O ral L iqu id,AOL)对家兔金葡菌性结膜炎及刺激性结膜炎的治疗作用。方法:建立兔眼损伤金葡菌性结膜炎及斑蝥酊刺激性结膜炎模型,观察各组动物的眼刺激症状、细菌转阴率及结膜的组织病理学改变,并和...目的:观察抗病毒口服液(Antivirus O ral L iqu id,AOL)对家兔金葡菌性结膜炎及刺激性结膜炎的治疗作用。方法:建立兔眼损伤金葡菌性结膜炎及斑蝥酊刺激性结膜炎模型,观察各组动物的眼刺激症状、细菌转阴率及结膜的组织病理学改变,并和氯霉素滴眼剂或舒目滴眼剂的治疗效果相比较。结果:1.AOL对家兔细菌性结膜炎具有明显抗炎作用,连续给药4 d后细菌转阴率>90%,其结膜充血及水肿症状比模型组明显减轻。2.AOL对刺激性兔眼结膜炎具有明显抗炎作用,给药1 d后眼结膜充血、水肿症状明显减轻;给药4 d后,其炎症已基本消失。结论:AOL对家兔金葡菌性结膜炎及刺激性结膜炎具有良好的疗效。展开更多
Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin (FN) from cultured rat mesangial cells and its possible mechanism. Methods: (1) MCs seeded at a density of 1 × 104 per well in 24-w...Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin (FN) from cultured rat mesangial cells and its possible mechanism. Methods: (1) MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34(10-12 mol/l -10-8 mol/l) for 6 h, 12 h, 24 h and 48 h, control cells were treated with vehicle only. The FN levels (in the supernatant) were measured by ELISA assay. (2) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34(10-12 mol/l - 10-8 mol/l ). Forty-eight hours later, FN were tested by ELISA. (4) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h, 12 h, 24 h and 48 h and then FN were tested. Results: (1) hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l (P<0. 01). (2) Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34, on synthesis of FN in cultured rat mesangial cells (P<0. 05). Conclusion: hPTH1-34 up-regulates FN synthesis in cultured rat mesangial cells via TGF-β, suggesting that PTH may play an important role in deteriorating the residual renal function at the early stage of chronic renal disease.展开更多
文摘目的:观察抗病毒口服液(Antivirus O ral L iqu id,AOL)对家兔金葡菌性结膜炎及刺激性结膜炎的治疗作用。方法:建立兔眼损伤金葡菌性结膜炎及斑蝥酊刺激性结膜炎模型,观察各组动物的眼刺激症状、细菌转阴率及结膜的组织病理学改变,并和氯霉素滴眼剂或舒目滴眼剂的治疗效果相比较。结果:1.AOL对家兔细菌性结膜炎具有明显抗炎作用,连续给药4 d后细菌转阴率>90%,其结膜充血及水肿症状比模型组明显减轻。2.AOL对刺激性兔眼结膜炎具有明显抗炎作用,给药1 d后眼结膜充血、水肿症状明显减轻;给药4 d后,其炎症已基本消失。结论:AOL对家兔金葡菌性结膜炎及刺激性结膜炎具有良好的疗效。
文摘Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin (FN) from cultured rat mesangial cells and its possible mechanism. Methods: (1) MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34(10-12 mol/l -10-8 mol/l) for 6 h, 12 h, 24 h and 48 h, control cells were treated with vehicle only. The FN levels (in the supernatant) were measured by ELISA assay. (2) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34(10-12 mol/l - 10-8 mol/l ). Forty-eight hours later, FN were tested by ELISA. (4) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h, 12 h, 24 h and 48 h and then FN were tested. Results: (1) hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l (P<0. 01). (2) Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34, on synthesis of FN in cultured rat mesangial cells (P<0. 05). Conclusion: hPTH1-34 up-regulates FN synthesis in cultured rat mesangial cells via TGF-β, suggesting that PTH may play an important role in deteriorating the residual renal function at the early stage of chronic renal disease.
