The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenc...The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenced. Together with the ITS sequences of 13 selected Rhododendron species and Bejaria racemosa downloaded from GenBank, we explored the infrageneric and sectional relationships of this important North Temperate genus by employing maximum-parsimony analysis using PAUP software. C. fastigiata and B. racemosa were designated as outgroups. The ITS-based tree inferred that: (1) Rhododendron was a well-supported monophyletic group, while subg. Therorhodion was basal to the rest of the genus; (2) Ledum was a member of Rhododendron, and its close relationship with the lepidote rhododendron was confirmed; (3) the lepidote rhododendron plus Ledum formed a strongly-supported monophyletic clade which was sister to the rest of the elepidote rhododendron; (4) the elepidote rhododendron formed a weakly-supported clade within which the monophyly of subg. Hynwrianthes and subg. Tsutsusi were strongly supported, while subg. Pentanthera and subg. Azaleastrum were polyphyletic; and (5) the monophyly of sect. Choniastnini, (subg. Azaleastrum) was strongly-supported, while subg. Tsutsusi could be sister to a weakly-supported clade composed of two sampled species of sect. Azaleastrum (subg. Azaleastrum) together with R. sentibarbatum, of subg. Mumeazalea.展开更多
[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） f...[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） from 29 taxa of Caragana species and seven close relatives （all belong to Astralinae （Adens） Benth） were used for phylogenetic analysis. [Result] Length of the entire ITS region ranges from 611 to 614 bp in Caragana species. The aligned sequences nrlTS of Caragana are 655 bp, and 170 sites are variable, with 107 phylogenetically informative sites. The phylogenetically informative sites are 16.3% of the total aligned sequences. The ITS sequences data are useful to resolve some relationships at lower taxonomic levels within Caragana. The Caragana Fabr. is not a monophyletic group with very close connection with Calophaca tianschanica. The ITS data revealed that the species of Sect. tragacanthoides were dispersed in MP tree or ME tree. Although the morphol- ogy of C. ordosica is similar to C. tibetica, the ITS results revealed an unexpectedly close relativeship to C. roborovskyi. The ITS data also indicate C. davazamcii, C. korshinskii, C. intermedia, and C. microphylla are different species. [Conclusion] ITS sequences have an important reference value in exploring the relationships of Cara- gana.展开更多
[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta ...[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced（COⅠ 1 539 bp,COⅡ 684 bp,CO Ⅲ 792 bp,with the total of 3 015 bp）.The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment.Phylogenic trees of four subspecies of L.migratoria were constructed by Neighbor-Joining,Maximum-parsimony and Bayesian,respectively.[Result] The average content of A ＋ T in three subunits of four subspecies was 69.57%;the third site of codon showed the highest A ＋ T content,and the COⅠ had the highest A ＋ T content（87.6%）;The nucleotide substitution mainly occurred at the third site of codon,and the nucleotide replacement rate of CO Ⅱ was the highest.The second site of codon was conservative,so the replacement rate was in the range of 5.9%-15%.The start codon of COⅠ was CCG or ACG.Genetic distances among four subspecies were ranged from 0.001 to 0.076.The relationship between L.m.tibetensis and Locusta migratoria manilensis was the closest,followed by L.m.migratorioides and L.m.migratorioides,while the genetic distance between L.m.tibetensis and L.m.migratorioides was the largest.[Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L.m.tibetensis,L.m.manilensis,L.m.migratoria,L.m.migratorioides.展开更多
Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representin...Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A+T content is 73.9%,ranging from 71.2% to 77.5%;the overall G+C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.展开更多
Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of IT...Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit （LSU） rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.展开更多
In recent years,multiple-load automatic guided vehicle(AGV)is increasingly used in the logistics transportation fields,owing to the advantages of smaller fleet size and fewer occurrences of traffic congestion.However,...In recent years,multiple-load automatic guided vehicle(AGV)is increasingly used in the logistics transportation fields,owing to the advantages of smaller fleet size and fewer occurrences of traffic congestion.However,one main challenge lies in the deadlock-avoidance for the dispatching process of a multiple-load AGV system.To prevent the system from falling into a deadlock,a strategy of keeping the number of jobs in the system(NJIS)at a low level is adopted in most existing literatures.It is noteworthy that a low-level NJIS will make the processing machine easier to be starved,thereby reducing the system efficiency unavoidably.The motivation of the paper is to develop a deadlock-avoidance dispatching method for a multiple-load AGV system operating at a high NJIS level.Firstly,the deadlock-avoidance dispatching method is devised by incorporating a deadlock-avoidance strategy into a dispatching procedure that contains four sub-problems.In this strategy,critical tasks are recognized according to the status of workstation buffers,and then temporarily forbidden to avoid potential deadlocks.Secondly,three multiattribute dispatching rules are designed for system efficiency,where both the traveling distance and the buffer status are taken into account.Finally,a simulation system is developed to evaluate the performance of the proposed deadlock-avoidance strategy and dispatching rules at different NJIS levels.The experimental results demonstrate that our deadlock-avoidance dispatching method can improve the system efficiency at a high NJIS level and the adaptability to various system settings,while still avoiding potential deadlocks.展开更多
To examine the importance of kin selection in shaping human societies, this study analyzed the kinship system practiced in traditional China for two millennia and teased apart its underlying genetic and other, presuma...To examine the importance of kin selection in shaping human societies, this study analyzed the kinship system practiced in traditional China for two millennia and teased apart its underlying genetic and other, presumably cultural, components. The results demonstrate that, in the traditional patrilineal Chinese family, both genetic relatedness and the cultural factor of generation were important in determining kinship status for male agnates （genetically related relatives）. For female agnates, however, only genetic relatedness was important. Another surprising finding was that the influence of gender was not as important as genetic relatedness. The most interesting finding in this study, however, was that kin selection and culture （i.e., seniority in generation and age） played vastly different roles in different lineages in the Chinese family： for collateral （indirect） agnates, genetic relatedness was the most important factor in determining their kinship status, but for lineal （direct） agnates, its importance was overridden by seniority in generation and age, a cultural factor. Several other bio-cultural factors also explained a considerable amount of variance in kinship status. Since kinship profoundly affected, and was often the foundation of, the legal and social systems in dynastic China, kin selection, while its strength may differ remarkably between lineal and collateral relatives, could act as a selective force in Chinese families展开更多
This thesis makes an analysis of the influence of the implementation of university personnel agency on three participators of universities, agency, and agency personnel. It is pointed out those personnel agency of the...This thesis makes an analysis of the influence of the implementation of university personnel agency on three participators of universities, agency, and agency personnel. It is pointed out those personnel agency of the current period is mainly transforming fi＇om ＂personnel relations agency＂ to ＂household register relation affiliation＂ in terms of initial system design intention. Finally, it proposes that we should further improve the existing university personnel agency system, so as to eliminate the adverse effects on all parties.展开更多
Detailed soil surveys involve costly and time-consuming work and require expert knowledge. Since soil surveys provide information to meet a wide range of needs, new methods are necessary to map soils quickly and accur...Detailed soil surveys involve costly and time-consuming work and require expert knowledge. Since soil surveys provide information to meet a wide range of needs, new methods are necessary to map soils quickly and accurately. In this study, multilayer perceptron artificial neural networks(ANNs) were developed to map soil units using digital elevation model(DEM) attributes. Several optimal ANNs were produced based on a number of input data and hidden units. The approach used test and validation areas to calculate the accuracy of interpolated and extrapolated data. The results showed that the system and level of soil classification employed had a direct effect on the accuracy of the results. At the lowest level, smaller errors were observed with the World Reference Base(WRB)classification criteria than the Soil Taxonomy(ST) system, but more soil classes could be predicted when using ST(7 soils in the case of ST vs. 5 with WRB). Training errors were below 11% for all the ANN models applied, while the test error(interpolation error) and validation error(extrapolation error) were as high as 50% and 70%, respectively. As expected, soil prediction using a higher level of classification presented a better overall level of accuracy. To obtain better predictions, in addition to DEM attributes, data related to landforms and/or lithology as soil-forming factors, should be used as ANN input data.展开更多
文摘The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenced. Together with the ITS sequences of 13 selected Rhododendron species and Bejaria racemosa downloaded from GenBank, we explored the infrageneric and sectional relationships of this important North Temperate genus by employing maximum-parsimony analysis using PAUP software. C. fastigiata and B. racemosa were designated as outgroups. The ITS-based tree inferred that: (1) Rhododendron was a well-supported monophyletic group, while subg. Therorhodion was basal to the rest of the genus; (2) Ledum was a member of Rhododendron, and its close relationship with the lepidote rhododendron was confirmed; (3) the lepidote rhododendron plus Ledum formed a strongly-supported monophyletic clade which was sister to the rest of the elepidote rhododendron; (4) the elepidote rhododendron formed a weakly-supported clade within which the monophyly of subg. Hynwrianthes and subg. Tsutsusi were strongly supported, while subg. Pentanthera and subg. Azaleastrum were polyphyletic; and (5) the monophyly of sect. Choniastnini, (subg. Azaleastrum) was strongly-supported, while subg. Tsutsusi could be sister to a weakly-supported clade composed of two sampled species of sect. Azaleastrum (subg. Azaleastrum) together with R. sentibarbatum, of subg. Mumeazalea.
基金Supported by National Key Basic Research Development Program of China(2002CB111505)~~
文摘[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） from 29 taxa of Caragana species and seven close relatives （all belong to Astralinae （Adens） Benth） were used for phylogenetic analysis. [Result] Length of the entire ITS region ranges from 611 to 614 bp in Caragana species. The aligned sequences nrlTS of Caragana are 655 bp, and 170 sites are variable, with 107 phylogenetically informative sites. The phylogenetically informative sites are 16.3% of the total aligned sequences. The ITS sequences data are useful to resolve some relationships at lower taxonomic levels within Caragana. The Caragana Fabr. is not a monophyletic group with very close connection with Calophaca tianschanica. The ITS data revealed that the species of Sect. tragacanthoides were dispersed in MP tree or ME tree. Although the morphol- ogy of C. ordosica is similar to C. tibetica, the ITS results revealed an unexpectedly close relativeship to C. roborovskyi. The ITS data also indicate C. davazamcii, C. korshinskii, C. intermedia, and C. microphylla are different species. [Conclusion] ITS sequences have an important reference value in exploring the relationships of Cara- gana.
基金Supported by National Natural Science Foundation of China(30770263)~~
文摘[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced（COⅠ 1 539 bp,COⅡ 684 bp,CO Ⅲ 792 bp,with the total of 3 015 bp）.The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment.Phylogenic trees of four subspecies of L.migratoria were constructed by Neighbor-Joining,Maximum-parsimony and Bayesian,respectively.[Result] The average content of A ＋ T in three subunits of four subspecies was 69.57%;the third site of codon showed the highest A ＋ T content,and the COⅠ had the highest A ＋ T content（87.6%）;The nucleotide substitution mainly occurred at the third site of codon,and the nucleotide replacement rate of CO Ⅱ was the highest.The second site of codon was conservative,so the replacement rate was in the range of 5.9%-15%.The start codon of COⅠ was CCG or ACG.Genetic distances among four subspecies were ranged from 0.001 to 0.076.The relationship between L.m.tibetensis and Locusta migratoria manilensis was the closest,followed by L.m.migratorioides and L.m.migratorioides,while the genetic distance between L.m.tibetensis and L.m.migratorioides was the largest.[Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L.m.tibetensis,L.m.manilensis,L.m.migratoria,L.m.migratorioides.
文摘Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A+T content is 73.9%,ranging from 71.2% to 77.5%;the overall G+C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.
基金Project (No.2002C34003) supported by the Science and Technology Department of Zhejiang Province,China
文摘Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit （LSU） rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.
基金supported by the National Natural Science Foundation of China(Nos.52005427,61973154)the National Defense Basic Scientific Research Program of China(No.JCKY2018605C004)+1 种基金the Natural Science Research Project of Jiangsu Higher Education Institutions(Nos.19KJB510013,18KJA460009)the Foundation of Graduate Innovation Center in Nanjing University of Aeronautics and Astronautics(No.KFJJ20190516)。
文摘In recent years,multiple-load automatic guided vehicle(AGV)is increasingly used in the logistics transportation fields,owing to the advantages of smaller fleet size and fewer occurrences of traffic congestion.However,one main challenge lies in the deadlock-avoidance for the dispatching process of a multiple-load AGV system.To prevent the system from falling into a deadlock,a strategy of keeping the number of jobs in the system(NJIS)at a low level is adopted in most existing literatures.It is noteworthy that a low-level NJIS will make the processing machine easier to be starved,thereby reducing the system efficiency unavoidably.The motivation of the paper is to develop a deadlock-avoidance dispatching method for a multiple-load AGV system operating at a high NJIS level.Firstly,the deadlock-avoidance dispatching method is devised by incorporating a deadlock-avoidance strategy into a dispatching procedure that contains four sub-problems.In this strategy,critical tasks are recognized according to the status of workstation buffers,and then temporarily forbidden to avoid potential deadlocks.Secondly,three multiattribute dispatching rules are designed for system efficiency,where both the traveling distance and the buffer status are taken into account.Finally,a simulation system is developed to evaluate the performance of the proposed deadlock-avoidance strategy and dispatching rules at different NJIS levels.The experimental results demonstrate that our deadlock-avoidance dispatching method can improve the system efficiency at a high NJIS level and the adaptability to various system settings,while still avoiding potential deadlocks.
文摘To examine the importance of kin selection in shaping human societies, this study analyzed the kinship system practiced in traditional China for two millennia and teased apart its underlying genetic and other, presumably cultural, components. The results demonstrate that, in the traditional patrilineal Chinese family, both genetic relatedness and the cultural factor of generation were important in determining kinship status for male agnates （genetically related relatives）. For female agnates, however, only genetic relatedness was important. Another surprising finding was that the influence of gender was not as important as genetic relatedness. The most interesting finding in this study, however, was that kin selection and culture （i.e., seniority in generation and age） played vastly different roles in different lineages in the Chinese family： for collateral （indirect） agnates, genetic relatedness was the most important factor in determining their kinship status, but for lineal （direct） agnates, its importance was overridden by seniority in generation and age, a cultural factor. Several other bio-cultural factors also explained a considerable amount of variance in kinship status. Since kinship profoundly affected, and was often the foundation of, the legal and social systems in dynastic China, kin selection, while its strength may differ remarkably between lineal and collateral relatives, could act as a selective force in Chinese families
文摘This thesis makes an analysis of the influence of the implementation of university personnel agency on three participators of universities, agency, and agency personnel. It is pointed out those personnel agency of the current period is mainly transforming fi＇om ＂personnel relations agency＂ to ＂household register relation affiliation＂ in terms of initial system design intention. Finally, it proposes that we should further improve the existing university personnel agency system, so as to eliminate the adverse effects on all parties.
文摘Detailed soil surveys involve costly and time-consuming work and require expert knowledge. Since soil surveys provide information to meet a wide range of needs, new methods are necessary to map soils quickly and accurately. In this study, multilayer perceptron artificial neural networks(ANNs) were developed to map soil units using digital elevation model(DEM) attributes. Several optimal ANNs were produced based on a number of input data and hidden units. The approach used test and validation areas to calculate the accuracy of interpolated and extrapolated data. The results showed that the system and level of soil classification employed had a direct effect on the accuracy of the results. At the lowest level, smaller errors were observed with the World Reference Base(WRB)classification criteria than the Soil Taxonomy(ST) system, but more soil classes could be predicted when using ST(7 soils in the case of ST vs. 5 with WRB). Training errors were below 11% for all the ANN models applied, while the test error(interpolation error) and validation error(extrapolation error) were as high as 50% and 70%, respectively. As expected, soil prediction using a higher level of classification presented a better overall level of accuracy. To obtain better predictions, in addition to DEM attributes, data related to landforms and/or lithology as soil-forming factors, should be used as ANN input data.
