软件企业是知识密集型企业,知识成为企业重要的生产资源。软件企业在开发软件的过程中,越来越重视利用过去的经验,重用以前某些相似项目的分析、设计、代码等。维基技术是近年来发展起来的一种超文本系统的新技术,维基技术可以帮助软件...软件企业是知识密集型企业,知识成为企业重要的生产资源。软件企业在开发软件的过程中,越来越重视利用过去的经验,重用以前某些相似项目的分析、设计、代码等。维基技术是近年来发展起来的一种超文本系统的新技术,维基技术可以帮助软件企业建立知识库。软件企业在局域网基础上应用 Wiki 技术可以辅助组织实现其内部的知识共享。展开更多
Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular...Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.展开更多
In this short paper we generalize the conditions that Casoratian entries satisfy for the two-dlmenslonal Toda lattice. Although we finally conclude that our generalization is trivial in some sense for getting new solu...In this short paper we generalize the conditions that Casoratian entries satisfy for the two-dlmenslonal Toda lattice. Although we finally conclude that our generalization is trivial in some sense for getting new solutions, our discussion is still helpful for the study of Wronskian technique.展开更多
Pichia pastoris expression system is similar to that of the mammal cell in modification of expressed protein, including refolding and glycosylation. A human aFGF gene was cloned into the intracellular expression vecto...Pichia pastoris expression system is similar to that of the mammal cell in modification of expressed protein, including refolding and glycosylation. A human aFGF gene was cloned into the intracellular expression vector pPIC9K. The Pichia pastoris KM71 strain was transformed with the recombined expression plasmid. Transgenic expression was observed after screening the transformants with G418. The expression and secretion of recombinant human aFGF (rhaFGF) into the culture medium were testified by ELISA assay. The yield peaked after two days of induction and was approximately 10 mgL-1 in shake-flask fermentation medium. The recombinant proteins were purified by the combination of heparin-Sepharose affinity chromatography and gel filtration chromatography. Two proteins with relative molecular masses (Mr) of 17 000 and 35 000 were purified as a single band in SDS-PAGE, whose biological activities were determined by MTT assay. It is found that the protein with Mr of 17 000 is nonglycosylated haFGF, and that with Mr of 35 000 is glycosylated haFGF; and the latter has a lower biological activity than the former.展开更多
Currently,with the fast development of GPS technique,GPS RTK is rapidly being applied to the cadastral survey. GPS RTK technique not only improves efficiency and accelerates the rate of mapping,but also increases econ...Currently,with the fast development of GPS technique,GPS RTK is rapidly being applied to the cadastral survey. GPS RTK technique not only improves efficiency and accelerates the rate of mapping,but also increases economic efficiency. The authors introduced a method for GPS RTK rapid positioning,which uses continuous operational reference system (CORS) as a base station to change the method of previous map drawing by obtaining real-time three-dimensional coordinates of boundary points and statistically analyzing with the three-dimensional coordinates obtained by Total Station to get the accuracy of GPS RTK real-time coordinates. The application of GPS RTK in practical measurement was discussed when the data link had been lost especially. The results show that the three-dimensional coordinates measured by total station can be replaced by real-time three-dimensional coordinates measured by Engineering Star RTK in the CORS.展开更多
Bridges are important elements in road system and can influence the entire economy of cities and region. Usually, these structures have high financial investments for their constructions, in this way, maintenance and ...Bridges are important elements in road system and can influence the entire economy of cities and region. Usually, these structures have high financial investments for their constructions, in this way, maintenance and conservation become so important. Inspection is a technical activity that covers several operations, including performance analysis; examination; final performance report, other operations may be necessary, such as maintenance work, recovery, strengthening and rehabilitation. Technical examination together with some test methods allows a critical and parametric judgment of the bridge performance by minimizing the subjectivity of visual evaluations, and permits a more detailed diagnosis. This paper discusses the methodology to perform tests to complement the assessment recommended by DNIT （Brazilian National Department of Transport Infrastructure）, which consists only on a visual judgment. This approach provides technical basis to classify the bridges as its need and urgency of maintenance.展开更多
The use of three-dimensional(3D)electrodes in water treatment is competitive because of their high catalytic efficiency,low energy consumption and promising development.The use of particle electrodes is a key research...The use of three-dimensional(3D)electrodes in water treatment is competitive because of their high catalytic efficiency,low energy consumption and promising development.The use of particle electrodes is a key research focus in this technology.They are usually in the form of particles that fill the space between the cathode and anode,and the selection of materials used is important.Carbon-based materials are widely used because of their large specific surface area,good adsorption performance,high chemical stability and low cost.The principles of 3D electrode technology are introduced and recent research on its use for degrading organic pollutants using carbon-based particle electrodes is summarized.The classification of particle electrodes is introduced and the challenges for the future development of carbon-based particle electrodes in wastewater treatment are discussed.展开更多
文摘软件企业是知识密集型企业,知识成为企业重要的生产资源。软件企业在开发软件的过程中,越来越重视利用过去的经验,重用以前某些相似项目的分析、设计、代码等。维基技术是近年来发展起来的一种超文本系统的新技术,维基技术可以帮助软件企业建立知识库。软件企业在局域网基础上应用 Wiki 技术可以辅助组织实现其内部的知识共享。
基金supported by the National Natural Science Foundation of China (11372243, 11532009, and 11522219)the China Postdoctoral Science Foundation (2013M540742)+2 种基金the Doctoral Program of Higher Education of China (20130201120071)the Natural Science Basic Research Plan in Shaanxi Province of China (2014JQ1004)the Fun- damental Research Funds for the Central Universities
文摘Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.
基金＊The project supported by National Natural Science Foundation of China under Grant No. 10371070, the Youth Foundation of Shanghai Education Committee, and the Foundation of Shanghai Education Committee for Shanghai Prospective Excellent Young Teachers
文摘In this short paper we generalize the conditions that Casoratian entries satisfy for the two-dlmenslonal Toda lattice. Although we finally conclude that our generalization is trivial in some sense for getting new solutions, our discussion is still helpful for the study of Wronskian technique.
基金the National Natural Science Foundation of China (No.19732003)
文摘Pichia pastoris expression system is similar to that of the mammal cell in modification of expressed protein, including refolding and glycosylation. A human aFGF gene was cloned into the intracellular expression vector pPIC9K. The Pichia pastoris KM71 strain was transformed with the recombined expression plasmid. Transgenic expression was observed after screening the transformants with G418. The expression and secretion of recombinant human aFGF (rhaFGF) into the culture medium were testified by ELISA assay. The yield peaked after two days of induction and was approximately 10 mgL-1 in shake-flask fermentation medium. The recombinant proteins were purified by the combination of heparin-Sepharose affinity chromatography and gel filtration chromatography. Two proteins with relative molecular masses (Mr) of 17 000 and 35 000 were purified as a single band in SDS-PAGE, whose biological activities were determined by MTT assay. It is found that the protein with Mr of 17 000 is nonglycosylated haFGF, and that with Mr of 35 000 is glycosylated haFGF; and the latter has a lower biological activity than the former.
文摘Currently,with the fast development of GPS technique,GPS RTK is rapidly being applied to the cadastral survey. GPS RTK technique not only improves efficiency and accelerates the rate of mapping,but also increases economic efficiency. The authors introduced a method for GPS RTK rapid positioning,which uses continuous operational reference system (CORS) as a base station to change the method of previous map drawing by obtaining real-time three-dimensional coordinates of boundary points and statistically analyzing with the three-dimensional coordinates obtained by Total Station to get the accuracy of GPS RTK real-time coordinates. The application of GPS RTK in practical measurement was discussed when the data link had been lost especially. The results show that the three-dimensional coordinates measured by total station can be replaced by real-time three-dimensional coordinates measured by Engineering Star RTK in the CORS.
文摘Bridges are important elements in road system and can influence the entire economy of cities and region. Usually, these structures have high financial investments for their constructions, in this way, maintenance and conservation become so important. Inspection is a technical activity that covers several operations, including performance analysis; examination; final performance report, other operations may be necessary, such as maintenance work, recovery, strengthening and rehabilitation. Technical examination together with some test methods allows a critical and parametric judgment of the bridge performance by minimizing the subjectivity of visual evaluations, and permits a more detailed diagnosis. This paper discusses the methodology to perform tests to complement the assessment recommended by DNIT （Brazilian National Department of Transport Infrastructure）, which consists only on a visual judgment. This approach provides technical basis to classify the bridges as its need and urgency of maintenance.
文摘The use of three-dimensional(3D)electrodes in water treatment is competitive because of their high catalytic efficiency,low energy consumption and promising development.The use of particle electrodes is a key research focus in this technology.They are usually in the form of particles that fill the space between the cathode and anode,and the selection of materials used is important.Carbon-based materials are widely used because of their large specific surface area,good adsorption performance,high chemical stability and low cost.The principles of 3D electrode technology are introduced and recent research on its use for degrading organic pollutants using carbon-based particle electrodes is summarized.The classification of particle electrodes is introduced and the challenges for the future development of carbon-based particle electrodes in wastewater treatment are discussed.
