莲心碱冻干粉针抗心律失常药效学研究

目的研究莲心碱冻干粉针抗心律失常的药效学。方法采用哇巴因、氯化钙、乌头碱、氯仿诱导心律失常动物模型 ,分别观察不同剂量组的莲心碱冻干粉针抗心律失常的作用。结果高、中、低剂量的莲心碱冻干粉针均能显著提高哇巴因和乌头碱致大鼠室性早搏 (VE)、室性纤颤 (VF)及心脏停搏 (CA)时的用量 ;延长氯化钙诱发大鼠心律失常出现的时间 ,缩短生存大鼠的窦性心律恢复时间 ,减少死亡率 ;降低氯仿所致小鼠室颤发生率 ,延长小鼠出现室颤的时间。

钙通道阻断剂在围术期的应用

临床常用的三种钙通道阻断剂维拉帕咪、地尔硫卓及二羟吡啶类药物，通过作用位于心肌、周围动脉血管平滑肌和神经系统细胞膜上的L型和N型钙离子通道而发挥临床作用。由于上述三种钙通道阻断剂在心肌和周围动脉血管平滑肌的选择性方面存在显著差异（二羟吡啶类药物-尼卡地平对周围动脉血管与心肌的选择性为30，000倍，而地尔硫卓为70倍，维拉帕咪仅为3倍），

Effects of 2-[p-(Dimethylamino)Styrl] PyridineMethiodide on Slow Response Action Potentialand Slow lnward Current of Guinea PigPapillary Muscles

The effects of 2-[p-(Dimethylamino)styryl] pyridine methiodide(DSPM)on slow response action potential(SRAP)and slow inward current(Isi) of guinea pig papillary muscles were studied by intracellular microelectrodes and voltage clamp techniques.The APA and V_(max) of SRAP induced by high K￣+were decreased after 50 min of perfusion with DSPM solu- tion. Isi was suppressed from a peak value of8.8± 1.6μA to 5.7± 1.8μA, The results indicated that DSPM has a selective blocking effect on calcium channel.

Antineoplastic Effect of Calcium Channel Blocker-Verapamil and 5-Fluorouracil Intraperitoneal Chemotherapy on Hepatocarcinoma-Bearing Rats

Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy onhepatocarcinoma-bearing rats, and examine the action between calcium channel blockers and cytotoxic drugs.Methods We adopted the method of subcapsular implantation of carcinoma tissues of walker-256 in the left liver lobe as a model of livercarcinoma-bearing rats. All experimental animals were divided into four groups. On the sixth day post implantation, in group A (controlgroup) 6 ml of saline was injected intraperitoneally once a day for 3 days. In group B (single chemotherapy group) 6 ml of 5-Fu 75 mg/kg was injected intraperitoneally once a day for 3 days. In group C (combination of treatment group) both 5-Fu (75 mg/kg) and verapamil(25 mg/kg) were administered simultaneously as in A and B. In group D (simple verapamil group) only 6 ml of verapamil (25 mg/kg)was administered as above.Results Compared with groups A, B and D, The volume of cancer and the contents of liver cancer DNA and protein were significantlyreduced. The rates of inhibiting cancer (89.9% in group C and 35.4% in group B) were significantly increased in group C. Group C hadsignificantly long survival time compared to groups A, B and D ( P < 0.05) . By light microscopy, a number of focal necroses were foundin cancer tissue in group C.Conclusion Calcium channel blockers can enhance the antineoplastic effect of 5-Fu intraperitoneal chemotherapy to liver cancer ; Theuse of verapamil can not increase the toxicity of 5-Fu.

Effects of Berbamine on PAF Production in Human Neutrophils and on Platelet Aggregation

The effects of berbamine, an alkaloid of dibenzylisoquinoline, on PAF produc tion in human neutrophils and on platelet aggregation induced by PAF were studied and compared with those of the calcium antagonist verapamil. Preincubation with berbamine (50 mmol / L, 100 mmol / L) or verapamil (10 mmol / L, 100 mmol / L) was shown to significantly inhibit A 23187 stimulated PAF synthesis. Berbamine and verapamil were found to inhibit platelet aggregation induced by PAF 70 pmol / L in a dose dependent manner. These results suggest that the inhibitory effects of berbamine and verapamil on A 23187 stimulated PAF synthesis in human neutrophils and PAF induced platelet aggregation are possibly brought about by inhibiting cellular calcium influx.

异博定治疗高血压病45例临床观察

按1979年兰州会议标准,使用异博定治疗45例高血压病,每天总剂量240mg,疗程定为8周。对高血压病Ⅰ、Ⅱ期患者有效率为95.6％,显效率为82.2％。治疗前后对比,血压下降均值为3.3/2.3kPa,经方差分析(P>0.001);心率改变不大(P>0.0)5;血酯、血糖、肾功能均无影响。副作用小,优于噻嗪类利尿剂和β-阻滞剂,可作为高血压病阶梯治疗的第一线药物。