Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (...Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6～8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC_3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6～8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels.展开更多
In petrochemical plant, the in-operation repairing is usually a repairing strategy with pressured inoperation repairing for avoiding huge economic losses caused by unplanned shutdown when some slight local leakage hap...In petrochemical plant, the in-operation repairing is usually a repairing strategy with pressured inoperation repairing for avoiding huge economic losses caused by unplanned shutdown when some slight local leakage happens in pipes. This paper studies the effects of repairing strategies on the failure probability of the pipe systems in process industries based on the time-average fault tree approach, especially the in-operation repairing strategies including pressured in-operation repairing activities. The fault tree model can predict the effect of different repairing plans on the pipe failure probability, which is significant to the optimization of the repairing plans. At first pipes are distinguished into four states in this model, i.e., successive state, flaw state, leakage state and failure state. Then the fault tree approach, which is usually applied in the studies of dynamic equipment, is adopted to model the pipe failure. Moreover, the effect of pressured in-operation repairing is also considered in the model. In addition, this paper proposes a series of time-average parameters of the fault tree model, all of which are used to calculate node parameters of the fault tree model. At last, a practical case is calculated based on the fault tree model in a repairing activity of pipe thinning.展开更多
In this paper, based on the analysis and test methods of national standards （GB 14754-2010） and chemical analysis and test items carried out by chemical enterprises, a set of automatic processing of quality analysis...In this paper, based on the analysis and test methods of national standards （GB 14754-2010） and chemical analysis and test items carried out by chemical enterprises, a set of automatic processing of quality analysis test data of ascorbic acid products was developed by using access database technology and Visual Basic programming language system, and its stability was investigated. The results show that the software can manage intelligently all aspects of the quality analysis and test of ascorbic acid products, uploading timely the data and results of the analysis and inspection to the network and saving it for users, enterprises and quality management, which set up a network of information sharing platform to ensure the authenticity and reliability of measurement results, improving greatly the speed of data processing, saving valuable time, reducing production costs with good economic efficiency and social benefit. It has practical value for ascorbic acid quality analysis test data processing automatically the results of the implementation of intelligent management.展开更多
基金supported by the national“973”tissue engineering project of China(G1999054300)Shanghai Science and Technology Development Foundation(03DJ14021)
文摘Endothelial cells (TEC_3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × 10~6 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6～8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC_3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6～8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels.
基金Supported by National Science and Technology Pillar Program in the Twelfth Five-Year Plan (No. 2011BAK06B02)National Basic Research Program of China ("973" Program, No. 2012CB026000)
文摘In petrochemical plant, the in-operation repairing is usually a repairing strategy with pressured inoperation repairing for avoiding huge economic losses caused by unplanned shutdown when some slight local leakage happens in pipes. This paper studies the effects of repairing strategies on the failure probability of the pipe systems in process industries based on the time-average fault tree approach, especially the in-operation repairing strategies including pressured in-operation repairing activities. The fault tree model can predict the effect of different repairing plans on the pipe failure probability, which is significant to the optimization of the repairing plans. At first pipes are distinguished into four states in this model, i.e., successive state, flaw state, leakage state and failure state. Then the fault tree approach, which is usually applied in the studies of dynamic equipment, is adopted to model the pipe failure. Moreover, the effect of pressured in-operation repairing is also considered in the model. In addition, this paper proposes a series of time-average parameters of the fault tree model, all of which are used to calculate node parameters of the fault tree model. At last, a practical case is calculated based on the fault tree model in a repairing activity of pipe thinning.
文摘In this paper, based on the analysis and test methods of national standards （GB 14754-2010） and chemical analysis and test items carried out by chemical enterprises, a set of automatic processing of quality analysis test data of ascorbic acid products was developed by using access database technology and Visual Basic programming language system, and its stability was investigated. The results show that the software can manage intelligently all aspects of the quality analysis and test of ascorbic acid products, uploading timely the data and results of the analysis and inspection to the network and saving it for users, enterprises and quality management, which set up a network of information sharing platform to ensure the authenticity and reliability of measurement results, improving greatly the speed of data processing, saving valuable time, reducing production costs with good economic efficiency and social benefit. It has practical value for ascorbic acid quality analysis test data processing automatically the results of the implementation of intelligent management.
