子种绿A与锡反应分光光度法测定痕量锡

探讨了在氨性介质中子种绿Ａ（Ｃ３７Ｈ３５Ｎ２Ｏ６Ｓ２Ｎａ） 染料与锡（ＩＶ） 反应形成离子缔合物的最佳条件，建立了测定痕量锡的新方法。子种绿Ａ—Ｓｎ（ＩＶ） 缔合物的最大吸收波长位于６３０ ｎｍ ，表观摩尔吸光系数ε６３０ ＝ １．７×１０６ Ｌ／（ｍｏｌ·ｃｍ），锡浓度在０～８０ ｕｇ／Ｌ 范围内遵守比耳定律。用于水样与罐头食品分析，结果令人满意。

盐阜之子绿色情——记“全国绿化奖章”获得者、盐城市林业工作站站长戴蔚

心系绿化倾注情,造林护林做奉献。江苏省盐城市林业局林业工作站站长戴蔚,从事林业工作30个春秋,盐阜大地的每个林场、苗圃都留下了他的足迹。他把对苗木的爱洒在林地上,把对绿化的情倾注在苗圃里,创造了令人赞叹的成绩。

校园有把绿色的伞

校园的西南角,有个石头围砌的圆形花坛。坛内生长着一棵高大、粗壮的黄葛树。盛夏时节,那墨绿的树冠如同一把巨大的绿伞,遮蔽着小半个校园。春天带着魔力悄悄地来到校园。黄葛树睁开了蒙(目龙)的睡眼,在光秃秃的枝头上进出半粒豌豆大小的绿苞。我知道,这些绿苞过些时日就会成长一簇簇绿叶。于是,我心中充满了企盼。

解读《挪威的森林》中的三个女性形象

《挪威的森林》中主要刻画了直子、绿子、玲子三个女性形象,虽各不同,但在她们的身上有一个共同的特点,那就是都或多或少存在着精神方面的缺失。本文试借用弗洛伊德的人格结构论简要论述这三个女性的精神特质。

《挪威的森林》中孤独感的诗意描绘

在现代文明发展的过程中,随着城市的发展、扩大以及生活节奏的加快,人与人之间的交往也出现了很多阻隔,从而产生一种难以避免的孤独感。《挪威的森林》本是甲壳虫乐队的一首乐曲,这首乐曲忧伤、静谧,村上春树以此为自己的小说命名,使整个小说仿佛一首和谐、优美而又孤独的旋律,将都市青年难以名状的孤独感表现得淋漓尽致。在《挪威的森林》这部小说中,村上春树寄寓了自己内心强烈的孤独感以及对待孤独的态度。

Plasma methods for preparing green catalysts: Current status and perspective

Most current catalyst preparation methods cause pollution to air, water and land with the use of hazardous chemicals, lengthy operation time, high energy input and excessive water usage. The development of green catalyst preparation is necessary to prevent and eliminate waste from each step of the catalyst preparation. We summarize recent progress in the application of cold plasmas for green catalyst preparation. Cold plasma preparation can reduce the catalyst size, improve the dispersion and enhance catalyst-support interaction with the use of less or no hazardous chemicals. These improvements also lead to the enhancement of catalyst activity and stability. An alternative room temperature electron reduction with a non-hydrogen plasma as an electron source was developed for the reduction of noble metal ions in which no hazardous chemical reducing agent or hydrogen was needed. This creates many opportunities for the development of supported catalysts with heat sensitive substrates, including metal organic frameworks （MOFs）, covalent organic framework（COFs）, high surface area carbon, peptide, DNA, proteins and others. A novel floating metal catalyst on a water（or solution） surface has been established. Template removal using low temperature cold plasmas also leads to the formation of high surface area porous materials with characteristics that are normally only obtainable with high temperature calcination, but sintering can be avoided. Micro combustion has been developed for the removal of carbon template using cold plasma. This is promising for preparing many structured oxides in a simple way with no use of auxiliary chemicals. Many opportunities exist for the use of cold plasmas to make multi-metallic oxides. Some future development ideas are addressed.

Chemical Compositions of Volatile Oil from Fruiting Body of Armillaria luteo-virens

The chemical compositions of volatile oil from fruiting body of Armillaria luteo-virens in Qinghai Province were firstly analyzed with GC-MS and its relevant compositions were detected by calculating chromatographic peak area with normalized method. 21 peaks were separated and 13 compositions were identified which were mainly unsaturated fatty acids, taking 97.1% of the total volatile oil.

A Proteinase from Mung Bean Sprouts That Inactivaties Soybean Trypsin Inhibitor

By 30% - 60% (NH4)(2)SO4 fractional precipitation, anion-exchange chromatography on DEAE-Sepharose CL-6B, gel filtration on Sephacryl S-200 and anion-exchange chromatography on Waters AP-1 column (Protein(PM)-Pak DEAE 15HR), a proteinase which can inactivate soybean trypsin inhibitor (STI) was purified from mung bean ( Vigna rabiata (L.) Wilezek) sprouts. Its molecular weight was estimated to be 29.8 kD by SDS-PAGE, and its K-m and V-max for STI were 769.2 N-alpha -benzoyl-L-arginine ethyl ester BAEE/mL and 115.3 BAEE . mL(-1) . min(-1) respectively. This proteinase was stable at temperatures lower than 50 degreesC and pH 6.5 - 8.5, and 90.91% STI activity of defatted soybean powder was inactivated by this preparation, with proteolytic activity 5 000 BAEE/mL at 50 degreesC and pH 8.0 in 4 h.

Effects of High K^+ and Alkaline pH on Ultrastructure of Dunaliella salina Chloroplasts

ItwasreportedthatthegrowthofDunaliellasalinaTeod .culturedinmediumcontaining 1mol/L NaClwasalmostcompletelyinhibitedbytheadditionof 10 0mmol/LKCl.ThehighK+ (10 0mmol/LKCl) treatmentalsosignificantlyinhibitedthephotosyntheticrateofD .salinaanddecreasedchlorophyllcontentsin algae .ThisstudyfocusesonpossibleeffectsofhighK+ oralkalinepHontheultrastructuralchangeofchloro plastsinD .salina .AfterD .salinawasculturedinamediumcontaining 10 0mmol/LKClorinamedium withalkalinepHfor 8to 10days ,dramaticultrastructuralchangesoccurredinthechloroplastsincludingthy lakoidswelling ,volumeincreaseofchloroplast,andsignificantaccumulationofstarchgrainsinchloroplasts . Theresultsareconsistentwithourpreviousreportindicatingthattheultrastructuralchangesinchloroplastun derhighK+ oralkalinepHmayleadtoaninhibitoryeffectsonphotosynthesisandoverallgrowthofD .sali na .

Grey Relational Analysis between Chlorophyll a and Environmental Factors in ShaHu Lake

[Objective] The aim was to explore effects of environmental factors on the content of Chlorophyll a in ShaHu Lake.[Method] Based on the data in Shahu Lake from November in 2007 to September in 2008,the relationship between chlorophyll a and environmental factors like water temperature,pH,secchi-depth （SD）,total nitrogen,total phosphorus and potassium permanganate index was studied by grey relational analysis method.[Result] The main environmental factors affecting the content of Chlorophyll a in ShaHu Lake were in order of water temperature potassium permanganate index 〉total nitrogen 〉pH〉 total phosphorus 〉SD.[Conclusion] The research provides reference for the control of eutrophication and the reasonable development and utilization of Shahu Lake.

格高调雅——读井上靖的《猎枪》

井上靖先生(l 907--),是日本当代最著名的作家之一。生于北海进的旭川。J936年毕业于京都大学析学系,曾任《侮日新闻社》记者,1964年被选为日本艺术院会员,并先后获芥川奖、艺术院奖、文部大臣奖。现任日中文化交流协会会长。自1957年多次访华,对中扫友好做出了显著贡献。

都市中的森林与沙漠——读《挪威的森林》有感

“挪威的森林”(NORWEGIANWOOD)是60年代 Beatles 的一支忧伤的乐曲,尤其是在 John Lennon 那平淡却见沧桑的声音娓娓道来的演绎下,更令人莫名的沉醉。日本当代作家村上春树的小说《挪威的森林》就是从这首歌开场。主人公,37岁的“我”在汉堡机场着陆后,听到扩音器传来的一曲《挪威的森林》,其身心受到强烈的摇撼,难以自持,跌入了回忆世界……故事由此展开。

Enhancement of Photophosphorylation and Photosynthesis in Rice by Low Concentrations of NaHSO_3 Under Field Conditions

Spraying 1-2 mmol/L solution of NaHSO 3 on rice ( Oryza sativa L.) leaves resulted in the enhancement of net photosynthetic rate for more than three days. It was also observed that NaHSO 3 application caused increases both in ATP content in leaves and the millisecond_delayed light emission of leaves. The increase in net photosynthetic rate caused by NaHSO 3 treatment was similar to that by PMS (phenazine methosulfate) treatment. The grain yield of treated rice was enhanced approximately by 10% after duplicated application of NaHSO 3 in milk_ripening stage. It is suggested that the enhancement of photosynthesis by NaHSO 3 treatment resulted from the effect of increasing ATP supplement. Concomitant with an increase in the photosynthetic rate and ATP content in leaves, the transient increase in chlorophyll fluorescence after the termination of actinic light, which could be used as an index of the cyclic electron flow, was also enhanced by low concentration of NaHSO 3 treatment. Basing on these results it is proposed that the increase in rice photosynthesis caused by low concentrations of NaHSO 3 could be due to the stimulation of the cyclic electron flow around PSⅠ which in turn the enhancement of the coupled photophosphorylation and photosynthesis.

DNA Extraction and Molecular Identification of Green Tea

[Objective] The aim of this study was to provide reference for the quality identification of green tea.[Method] Green Tea was used as materials,and its total DNA was extracted through improved CTAB method.And the obtained DNA was used to carry out identification on 10 varieties of green tea through ISSR molecular markers.[Result] The high quality DNA from green tea could be obtained with new method,the DNA yield ranged from 101-498 μg/g tea sample for various green tea samples,and the average yield was 249 μg/g tea sample.The ISSR detection result showed that ISSR markers could effectively differentiate different varieties of green tea.[Conclusion] The result had provided reference for the further study on molecular identification of green tea.

Construction of Double Cross-over Expression Vector for Chloroplast Multicistron in Brassica napus L.

[Objective] This study aimed to construct Brassica napus chloroplast multi- cistron double cross-over expression vector, to lay the foundation for the genetic engi- neering research of Brassica napus chloroplast. [Method] Two primers were designed based on the known Brassica napus chloroplast DNA sequences AF267640 and Z50868 in GenBank. By using PCR method, two Brassica napus L. chloroplast DNA fragments were obtained, which were named RbcL and ACCD. The two Brassica na- pus chloroplast DNA homologous fragments were then cloned into plasmid pMD18-T to obtain recombinant plasmid pHBM715. Tandem expression cassette harboring spectinomycin-resistant gene aadA, mannanase gene man and green fluorescent pro- tein gene gfp was cloned into the plasmid pHBM715, thereby constructing Brassica napus chloroplast multicistron double cross-over expression vector pHBM716, which was transformed into Escherichia coil for expression and identification. [Result] Plate qualitative analysis was conducted for the functional identification of expression cas- sette in the constructed Brassica napus chloroplast multicistron double cross-over ex- pression vector, results showed that the three genes of the same multicistron were all expressed in E. coil [Conclusion] This study successfully constructed Brassica napus chloroplast multicistron double cross-over expression vector, which laid the foundation for the genetic engineering of Brassica napus chloroplast.

Screening and Genetic Analysis of the Hydrogen Peroxide Sensitive Mutant hps12 of Arabidopsis thaliana

In order to investigate the mechanism of ROS signaling transduction in after-germination process, we screened a hydrogen peroxide sensitive mutant from EMS mutant library of Arabidopsis thaliana. Hydrogen peroxide sensitive 12（hps12）was screened from this library. Phenotype analysis indicated that hps12 had dwarf plant and shorter pod than wild type（WT）. The cotyledon greening rate of hps12 was decreased after being treated with 4 mmol/L H_2O_2. Furthermore, the isolated leaves of hps12 mutants showed more obvious symptoms of senescence than WT under the treatment of 10 mmol/L H_2O_2. Genetic analysis suggested that hps12 was a monogenic recessive mutant.

Influence of copper ions and calcium ions on adsorption of CMC on chlorite

The effects of copper ions and calcium ions on the depression of chlorite using CMC（carboxymethyl cellulose） as a depressant were studied through flotation tests,adsorption measurements,ζ potential tests and co-precipitation experiments.The results show that the electrostatic repulsion between the CMC molecules and the chlorite surfaces hinders the approach of the CMC to the chlorite while the presence of copper ions and calcium ions enhances the adsorption density of CMC.The action mechanisms of these two types of ions are different.Calcium ions can not adsorb onto the mineral surfaces,but they can interact with the CMC molecules,thus reducing the charge of the CMC and enhancing adsorption density.Copper ions can adsorb onto the mineral surfaces,which facilitates the CMC adsorption through acid/base interaction.The enhanced adsorption density is also attributed to the decreased electrostatic repulsion between the CMC and mineral surfaces as copper ions reduce the surface charge of both the mineral surfaces and the CMC molecules.

Effects of Doubled_CO_2 Concentration on Ultrastructure, Supramolecular Architecture and Spectral Characteristics of Chloroplasts from Wheat

Wheat ( Triticum aestivum L.) plants were grown under ambient and doubled_CO 2(plus 350 μL/L) concentration in cylindrical open_top chamber to examine their effects on the ultrastructure, supramolecular architecture, absorption spectrum and low temperature (77 K) fluorescence emission spectrum of the chloroplasts from wheat leaves. The results were briefly summarized as follows: (1) The wheat leaves possessed normally developed chloroplasts with intact grana and stroma thylakoid membranes; The grana intertwined with stroma thylakoid membranes and increased slightly in stacking degree and the width of granum, in spite of more accumulated starch grains within the chloroplasts than those in control; (2) The particle density in the stacked region of the endoplasmic fracture face (EFs) and protoplasmic fracture face (PFs) and in the unstacked region the endoplasmic fracture face (EFu) and the protoplasmic fracture face (PFu) was significantly higher than that of control. Furthermore, in some cases many more particles on EFs faces of thylakoid membranes appeared as a paracrystalline particle array; (3) The variations in the structure of chloroplasts were consistent with the absorption spectra and the low temperature (77 K) fluorescence emission spectra of the chloroplasts developed under the doubled_CO 2 concentration. Results indicate that the capability of light energy absorption of chloroplasts and regulative capability of excitation energy distribution between PSⅡ and PSⅠ were raised by doubled_CO 2 concentration. This is very favorable for final productivity of wheat.