绿甘蓝冰温实体库及普通冷藏库的贮藏对比研究

本文实验对象为绿甘蓝,将其分别贮藏在冰温库和普通冷藏库中进行实验,测得两种冷藏库下绿甘蓝的失重率、腐烂指数和商品率。并将两种冷藏库贮存下绿甘蓝的失重率、腐烂指数和商品率随贮藏时间的变化进行对比研究分析;得出冰温实体库的贮藏效果最佳。

U-M协同提取绿甘蓝中原花青素工艺的优化

以衡水本地产绿甘蓝为研究对象,利用超声波、旋转机械研磨作用协同提取绿甘蓝中原花青素,同时根据协同原理设计了超声波-旋转机械研磨协同设备示意图。通过单因素试验和响应面试验优化提取工艺。超声-旋转机械研磨协同提取绿甘蓝中原花青素优化工艺条件为:超声-旋转机械研磨时间33 min、液料比值40 mL/g、超声功率450 W、超声-旋转机械研磨温度44℃、乙醇体积分数46%、旋转机械研磨转速1560 r/min。在此优化条件下原花青素得率为7.248 mg/g。实际3次平行验证试验结果表明,响应面优化得到的数学模型相对误差仅为0.01%,模型高效可用。

缓释砂硒对绿甘蓝富硒作用及生长的影响

砂硒因沙子具有透气保水的特性,同时硒释放相对缓慢,能提供稳定的硒来源,是比较理想的富硒蔬果培养基质。为了探讨缓释砂硒对绿甘蓝富硒作用和生长的影响,该研究设置对照组(CK),实验组缓释砂硒(CT)、鸡粪和砂硒1∶1混合(CT1)、鸡粪和砂硒1∶2混合(CT2)和鸡粪(CT3) 5个处理。结果表明:CT、CT1和CT2处理的绿甘蓝硒含量比CK分别增加45%、61%和64%,逐步回归分析表明绿甘蓝硒含量和土壤硒含量呈显著正相关(P<0.05); CT2处理的效果最好,绿甘蓝的产量增加45%,叶片厚度增加22.78%,水分利用效率提高56.66%。土壤锰含量和硒含量共同解释了绿甘蓝生物量变化的72%,而土壤锌含量解释绿甘蓝水分利用效率变化的66%。这表明砂硒添加后,通过增加土壤硒含量提高绿甘蓝硒含量,砂硒和鸡粪配比更有效地提高了绿甘蓝硒含量并促进其生长。

绿甘蓝栽培技术及病虫害防治

绿甘蓝又称紫包甘蓝、赤球甘蓝,因为其颜色绿而得名,为十字花科芸薹二年生植物.其易种植产量高,经济效益好,受到广大菜农的欢迎.文章主要介绍了绿甘蓝栽培技术,并就其病虫害防治做出初步的探索.

Correlation Analysis of SPAD Value with Chlorophyll Content and Economic Yield Traits of Brassica napus L.

[Objective] The aim was to compare differences of SPAD value, chloro- phyll content, agronomic characters, economic characters and yield traits to analyze correlation of SPAD value with other indices and establish regression functions. [Method] Based on 34 Brassica napus L. varieties, SPAD value, chlorophyll content, agronomic characters, economic characters and yield traits were measured and re- gression functions were established according to correlations. [Result] SPAD value, chlorophyll content, agronomic and economic characters and yield traits all achieved significant level in differences among 34 varieties. Specifically, SPAD value was of extremely significant correlation with chlorophyll a and b, total chlorophyll and carotenoid, and the correlation from high to low was chl-b〉chl-z〉chl-a〉chl-x. SPAD value was of significantly positive correlation with total pod number per plant, plant height, seed number per pod, yield per plant and harvest yield, and of insignificant correlation with branch point height, effective branch number, pod density of main stem, and pod length. [Conclusion] It is simple and rapid to predict chlorophyll con- tent, economic characters and yields of Brassica napus L. with SPAD value and re- gression functions.

Construction of Double Cross-over Expression Vector for Chloroplast Multicistron in Brassica napus L.

[Objective] This study aimed to construct Brassica napus chloroplast multi- cistron double cross-over expression vector, to lay the foundation for the genetic engi- neering research of Brassica napus chloroplast. [Method] Two primers were designed based on the known Brassica napus chloroplast DNA sequences AF267640 and Z50868 in GenBank. By using PCR method, two Brassica napus L. chloroplast DNA fragments were obtained, which were named RbcL and ACCD. The two Brassica na- pus chloroplast DNA homologous fragments were then cloned into plasmid pMD18-T to obtain recombinant plasmid pHBM715. Tandem expression cassette harboring spectinomycin-resistant gene aadA, mannanase gene man and green fluorescent pro- tein gene gfp was cloned into the plasmid pHBM715, thereby constructing Brassica napus chloroplast multicistron double cross-over expression vector pHBM716, which was transformed into Escherichia coil for expression and identification. [Result] Plate qualitative analysis was conducted for the functional identification of expression cas- sette in the constructed Brassica napus chloroplast multicistron double cross-over ex- pression vector, results showed that the three genes of the same multicistron were all expressed in E. coil [Conclusion] This study successfully constructed Brassica napus chloroplast multicistron double cross-over expression vector, which laid the foundation for the genetic engineering of Brassica napus chloroplast.