To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a pro...To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a probe.The DNA was successfully constructed on a 6xHis-tagged prokaryotic expression plasmid.The non-GFP labeled MIM-I-BAR encoding plasmid was also constructed as a control. Being successfully transformed into BL21 （DE3 ）cells,the GFP-conjugated MIM-I-BAR （MIM-I-BAR-GFP ） exhibits strong visible fluorescence,and the expression product can be easily detected by visual inspection, a fluorescence microscope, Western blot or ultraviolet and visible spectrophotometer. Moreover, examination of expression efficiency under various culture conditions revealed that the MIM-I-BAR-GFP gene has a high protein yield at 10 ℃,but not at the culture temperature of 37 ℃.This property is much different from that of the non-fluorescent MIM-I-BAR gene. This optimal expression condition is also proved to be feasible for protein production in midi-scale. The fluorescent recombinant MIM-I-BAR-GFP protein can serve as a useful tool in scientific research, biomedical application and pharmaceutical development.展开更多
The characteristic of green detergents and it＇s difference with regular detergent was expounded in this paper, according to the kernel of green chemistry. The variation trends of Chinese mainstream consumer group sho...The characteristic of green detergents and it＇s difference with regular detergent was expounded in this paper, according to the kernel of green chemistry. The variation trends of Chinese mainstream consumer group show that the new mainstream consumer group are concerning more and more about household detergent, especially its safety to human and environment. Growing attention of the mainstream consumer group and the standard drafting units on detergent will promote the development of green detergents, however, there are still challenges for the development of green detergents.展开更多
Two types of mesocarbon microbeads(MCMB), one of which was green sphere and the other carbonized at 1000 ℃, were used to modify coal tar pitches, and their influences on the development of carbonaceous mesophase in c...Two types of mesocarbon microbeads(MCMB), one of which was green sphere and the other carbonized at 1000 ℃, were used to modify coal tar pitches, and their influences on the development of carbonaceous mesophase in coal tar pitches were investigated. Optical microscopy was used to observe the changes caused by the additives. The green MCMB could promote the growth of mesophase spheres. When the holding time at the final temperature was prolonged to 100 min, the green MCMB-modified pitch would produce excellent bulk mesophase. Carbonized MCMB prohibited the coalescence of mesophase spheres and was beneficial to forming super large mesophase spheres. In the green MCMB-modified pitch, mesophase spheres grew up using the added MCMB spheres as nuclei, i.e. the green MCMB continued to grow during heat-treatment, which accelerated the formation, growth, and coalescence of mesophase spheres. For carbonized MCMB, their active sites were passivated and they could not continue to grow. These inert spheres could aggregate around the newly-formed mesophase spheres, prohibiting the growth and coalescence of the mesophase spheres.展开更多
Green buildings should be sustainable, efficient, economical, and to improve the quality of life of users and nearby residents. Unfortunately, some architecture features fostered by energy and environmental certificat...Green buildings should be sustainable, efficient, economical, and to improve the quality of life of users and nearby residents. Unfortunately, some architecture features fostered by energy and environmental certifications do not comply with fire prevention codes. Several factors can influence a building＇s energy efficiency and fire safety, such as bigger or smaller glass areas, building material thermal inertia, and the use of sustainable solutions such as green roofs. In this work, a methodology for integrating energy efficiency and fire prevention in green buildings is presented. A case study comparing a full glass skin and a building with 40% window size is considered. Also, the methodology permits modifications for obtaining a safe and energy efficient building.展开更多
基金The National Basic Research Program of China(973Program)(No.2011CB933503)the National Natural Science Foundation of China for Key Project of International Cooperation(No.61420106012)China Postdoctoral Science Foundation(No.2013M541592)
文摘To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a probe.The DNA was successfully constructed on a 6xHis-tagged prokaryotic expression plasmid.The non-GFP labeled MIM-I-BAR encoding plasmid was also constructed as a control. Being successfully transformed into BL21 （DE3 ）cells,the GFP-conjugated MIM-I-BAR （MIM-I-BAR-GFP ） exhibits strong visible fluorescence,and the expression product can be easily detected by visual inspection, a fluorescence microscope, Western blot or ultraviolet and visible spectrophotometer. Moreover, examination of expression efficiency under various culture conditions revealed that the MIM-I-BAR-GFP gene has a high protein yield at 10 ℃,but not at the culture temperature of 37 ℃.This property is much different from that of the non-fluorescent MIM-I-BAR gene. This optimal expression condition is also proved to be feasible for protein production in midi-scale. The fluorescent recombinant MIM-I-BAR-GFP protein can serve as a useful tool in scientific research, biomedical application and pharmaceutical development.
文摘The characteristic of green detergents and it＇s difference with regular detergent was expounded in this paper, according to the kernel of green chemistry. The variation trends of Chinese mainstream consumer group show that the new mainstream consumer group are concerning more and more about household detergent, especially its safety to human and environment. Growing attention of the mainstream consumer group and the standard drafting units on detergent will promote the development of green detergents, however, there are still challenges for the development of green detergents.
基金National Natural Science Foundation of China (No 50172034)
文摘Two types of mesocarbon microbeads(MCMB), one of which was green sphere and the other carbonized at 1000 ℃, were used to modify coal tar pitches, and their influences on the development of carbonaceous mesophase in coal tar pitches were investigated. Optical microscopy was used to observe the changes caused by the additives. The green MCMB could promote the growth of mesophase spheres. When the holding time at the final temperature was prolonged to 100 min, the green MCMB-modified pitch would produce excellent bulk mesophase. Carbonized MCMB prohibited the coalescence of mesophase spheres and was beneficial to forming super large mesophase spheres. In the green MCMB-modified pitch, mesophase spheres grew up using the added MCMB spheres as nuclei, i.e. the green MCMB continued to grow during heat-treatment, which accelerated the formation, growth, and coalescence of mesophase spheres. For carbonized MCMB, their active sites were passivated and they could not continue to grow. These inert spheres could aggregate around the newly-formed mesophase spheres, prohibiting the growth and coalescence of the mesophase spheres.
文摘Green buildings should be sustainable, efficient, economical, and to improve the quality of life of users and nearby residents. Unfortunately, some architecture features fostered by energy and environmental certifications do not comply with fire prevention codes. Several factors can influence a building＇s energy efficiency and fire safety, such as bigger or smaller glass areas, building material thermal inertia, and the use of sustainable solutions such as green roofs. In this work, a methodology for integrating energy efficiency and fire prevention in green buildings is presented. A case study comparing a full glass skin and a building with 40% window size is considered. Also, the methodology permits modifications for obtaining a safe and energy efficient building.
