Soft matter has attracted extensive attention due to its special physical/chemical properties and holds great promise in many applications. However, obtaining a detailed understanding of both complex fluid and mass tr...Soft matter has attracted extensive attention due to its special physical/chemical properties and holds great promise in many applications. However, obtaining a detailed understanding of both complex fluid and mass transport in soft matter, especially in hierarchical porous media of biological tissues, still remains a huge challenge. Herein, inspired by fast tracer transport in loose connective tissues of living systems, we observed an interesting phenomenon of fast molecular transport in situ in an artificial hierarchical multiphase porous medium （a micrometer scale hydrophobic fiber network filled with nanometer scale hydrophilic porous medium）, which was simply fabricated through electro- spinning technology and polymerization. The transportation speed of molecules in the micrometer fiber network is larger than simple diffusion in nanometer media, which is better described by Fick＇s law. We further proved that the phenomenon is based on the nanoconfined air/water/solid interface around the micrometer hydrophobic fibers. We focus on the key factors, referring to SA, （the confined multiphase area around the microfibers） and Nc （the connectivity node degree of the skeletal portion in the nanometer hydrogel medium）. Next, a quantitative parameter, VTCM （transport chance mean-value）, was introduced to describe the molecular transport capability of the fiber network within hierarchical multiphase porous systems. These fundamental advances can be applied de novo to understand the process of so-called simple diffusion in biological systems, and even to re-describe many molecular events in biologically nanoconfined spaces.展开更多
Objective: To label human insulin-like growth factor-I （hIGF-I） eukaryotic expression vector with green fluorescent protein （GFP） for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into ...Objective: To label human insulin-like growth factor-I （hIGF-I） eukaryotic expression vector with green fluorescent protein （GFP） for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA3.1-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of （hIGF-1） and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions: hIGF-I eukaryotic expression vector has been successfully labeled with GFP.展开更多
基金This study was supported by the National Natural Science Foundation of China （No. 81141118） and the National Basic Research Program of China （973 Program） （Nos. 2012CB9333800 and 2012CB518506）.
文摘Soft matter has attracted extensive attention due to its special physical/chemical properties and holds great promise in many applications. However, obtaining a detailed understanding of both complex fluid and mass transport in soft matter, especially in hierarchical porous media of biological tissues, still remains a huge challenge. Herein, inspired by fast tracer transport in loose connective tissues of living systems, we observed an interesting phenomenon of fast molecular transport in situ in an artificial hierarchical multiphase porous medium （a micrometer scale hydrophobic fiber network filled with nanometer scale hydrophilic porous medium）, which was simply fabricated through electro- spinning technology and polymerization. The transportation speed of molecules in the micrometer fiber network is larger than simple diffusion in nanometer media, which is better described by Fick＇s law. We further proved that the phenomenon is based on the nanoconfined air/water/solid interface around the micrometer hydrophobic fibers. We focus on the key factors, referring to SA, （the confined multiphase area around the microfibers） and Nc （the connectivity node degree of the skeletal portion in the nanometer hydrogel medium）. Next, a quantitative parameter, VTCM （transport chance mean-value）, was introduced to describe the molecular transport capability of the fiber network within hierarchical multiphase porous systems. These fundamental advances can be applied de novo to understand the process of so-called simple diffusion in biological systems, and even to re-describe many molecular events in biologically nanoconfined spaces.
文摘Objective: To label human insulin-like growth factor-I （hIGF-I） eukaryotic expression vector with green fluorescent protein （GFP） for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA3.1-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of （hIGF-1） and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions: hIGF-I eukaryotic expression vector has been successfully labeled with GFP.
