新型编织型生物反应器内大量乳猪肝细胞的组织化培养

目的 使反应器内培养的肝细胞能在较长时间保持肝细胞的特殊功能和活力 ,提高生物反应器的功效并为反应器内储存和运输肝细胞提供可能。方法 将新生实验型小猪肝细胞与微载体共同培养 ,待肝细胞与微载体充分粘附形成微载体 球形聚集体后 ,将其置入新型编织型生物反应器的外腔 ,用培养液循环式人工毛细管培养系统进行培养 ,在培养液内加入氯化氨、醋胺酚检测生物反应器的转化功能 ,行无血清培养检测肝细胞的白蛋白的合成功能 ,观察肝细胞的酶漏出量。锥虫蓝染色观察肝细胞的活力 ,电镜观察其超微结构。结果 生物反应器内培养的肝细胞在 1周内能保持较高的活力 ,并保持着较高的氯化氨、醋胺酚的生物转化及白蛋白合成功能 ,酶的漏出量也少。肝细胞超微结构示内质网、线粒体等细胞器丰富 ,核内染色体分布均匀 ,肝细胞间的微绒毛形成胆小管样结构。结论 肝细胞与微载体及肝细胞之间的聚集 ,形成直径大小不等的肝细胞 微载体球形聚集体 ,这种由肝细胞重新结合而成的聚集体类似于体内的肝组织结构 ,在新型编织型生物反应器内的中空纤维网架的支持下 ,肝细胞聚集体均匀地分散其中 ,为肝细胞的生长提供了一个类似体内内环境的三维空间 ,因而在无氧合器供氧的情况下 ,肝细胞也能在 1周内保持较好的形态和功?

FI-KR-FAAS在线预浓集系统中螯合剂的选择

The selection of chelators in the pre concentration system Flow Injection Knotted Reactor Flame Atomic Absorption Spectrometry(FI KR FAAS) and their corresponding experimental conditions were studied. Dithione was found to have advantages over the other two chelators with a higher concentration efficiency. Interferences from alkali and alkaline earth elements were overcome; mutual interferences between heavy metals were avoided. Concentration factors of 23 4-69 3 were obtained, with the detection limits of 1 61-2 56 μg/L. The method developed was used to determine certified biological reference materials of human hair, pig liver and sea prawn, and the results agreed well with the certified values, relative standard deviations of the determinations were in the range of 2 10%-3 02%.