【目的】评价艾可清胶囊联合高效抗病毒逆转录疗法(highly active antiretroviral therapy,HAART)治疗艾滋病(AIDS)患者的有效性和安全性。【方法】将18例患者随机分为2组,每组9例。治疗组在HAART治疗的同时加用中药艾可清胶囊,对照组...【目的】评价艾可清胶囊联合高效抗病毒逆转录疗法(highly active antiretroviral therapy,HAART)治疗艾滋病(AIDS)患者的有效性和安全性。【方法】将18例患者随机分为2组,每组9例。治疗组在HAART治疗的同时加用中药艾可清胶囊,对照组只给予HAART治疗。分别在治疗前、治疗3个月和治疗6个月检测T淋巴细胞亚群,记录症状体征积分(积分1)和症状舌脉积分(积分2),观察体质量和生存质量(卡洛夫斯基积分,Karnovsky Score),检测血常规、肝功能、肾功能及血清淀粉酶以评价艾可清胶囊的安全性。【结果】两组T淋巴细胞亚群各项指标在治疗后均有所改善(P<0.05或P<0.01),但各评价点两组间比较无显著性差异(P>0.05)。治疗组治疗后各评价点证候、体征积分均较对照组下降(P<0.05),卡洛夫斯基积分及体质量较对照组增加(P<0.05或P<0.01)。加用艾可清胶囊未见血常规、肝肾功能及血清淀粉酶异常变化。【结论】艾可清胶囊可有效、安全地缓解因HAART引起的不良反应。展开更多
The human immunodeficiency virus type 1 （HIV-1） can interact with and exploit the host cellular machinery to replicate and propagate itself. Numerous studies have shown that the Mitogen-activated protein kinase （M...The human immunodeficiency virus type 1 （HIV-1） can interact with and exploit the host cellular machinery to replicate and propagate itself. Numerous studies have shown that the Mitogen-activated protein kinase （MAPK） signal pathway can positively regulate the replication of HIV-1, but exactly how each MAPK pathway affects HIV-1 infection and replication is not understood. In this study, we used the Extracellular signal-regulated kinase （ERK） pathway inhibitor, PD98059, the Jun N-terminal kinase （JNK） pathway inhibitor, SP600125, and the p38 pathway inhibitor, SB203580, to investigate the roles of these pathways in HIV-1 replication. We found that application of PD98059 results in a strong VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus and HIV-1NL4-3 virus inhibition activity. In addition, SB203580 and SP600125 also elicited marked VSV-G pseudotyped HIV-INL4-3 luciferase reporter virus inhibition activity but no HIV-1NL4-3 virus inhibition activity. We also found that SB203580 and SP600125 can enhance the HIV-1 inhibition activity of PD98059 when cells were treated with all three MAPK pathway inhibitors in combination. Finally, we show that HIV-1 virus inhibition activity of the MAPK pathway inhibitors was the result of the negative regulation of HIV-1 LTR promoter activity.展开更多
Buckling of submarine pipelines under thermal stress is one of the most important problems to be considered in pipeline design. And pipeline with initial imperfections will easily undergo failure due to global bucklin...Buckling of submarine pipelines under thermal stress is one of the most important problems to be considered in pipeline design. And pipeline with initial imperfections will easily undergo failure due to global buckling under thermal stress and internal pressure. Therefore, it is vitally important to study the global buckling of the submarine pipeline with initial imperfections. On the basis of the characteristics of the initial imperfections, the global lateral buckling of submarine pipelines was analyzed. Based on the deduced analytical solutions for the global lateral buckling, effects of temperature difference and properties of foundation soil on pipeline buckling were analyzed. The results show that the snap buckling is predominantly governed by the amplitude value of initial imperfection; the triggering temperature difference of Mode I for pipelines with initial imperfections is higher than that of Mode I1; a pipeline with a larger friction coefficient is safer than that with a smaller one; pipelines with larger initial imperfections are safer than those with smaller ones.展开更多
文摘【目的】评价艾可清胶囊联合高效抗病毒逆转录疗法(highly active antiretroviral therapy,HAART)治疗艾滋病(AIDS)患者的有效性和安全性。【方法】将18例患者随机分为2组,每组9例。治疗组在HAART治疗的同时加用中药艾可清胶囊,对照组只给予HAART治疗。分别在治疗前、治疗3个月和治疗6个月检测T淋巴细胞亚群,记录症状体征积分(积分1)和症状舌脉积分(积分2),观察体质量和生存质量(卡洛夫斯基积分,Karnovsky Score),检测血常规、肝功能、肾功能及血清淀粉酶以评价艾可清胶囊的安全性。【结果】两组T淋巴细胞亚群各项指标在治疗后均有所改善(P<0.05或P<0.01),但各评价点两组间比较无显著性差异(P>0.05)。治疗组治疗后各评价点证候、体征积分均较对照组下降(P<0.05),卡洛夫斯基积分及体质量较对照组增加(P<0.05或P<0.01)。加用艾可清胶囊未见血常规、肝肾功能及血清淀粉酶异常变化。【结论】艾可清胶囊可有效、安全地缓解因HAART引起的不良反应。
基金supported by the Key Projects in the National Science and Technology Pillar Program during the Eleventh Five-Year Plan Period of China (2008ZX10001-002)Major Science and Technology Innovation Cross Project of the Chinese Academy of Sciences (KSCX1-YW-10)
文摘The human immunodeficiency virus type 1 （HIV-1） can interact with and exploit the host cellular machinery to replicate and propagate itself. Numerous studies have shown that the Mitogen-activated protein kinase （MAPK） signal pathway can positively regulate the replication of HIV-1, but exactly how each MAPK pathway affects HIV-1 infection and replication is not understood. In this study, we used the Extracellular signal-regulated kinase （ERK） pathway inhibitor, PD98059, the Jun N-terminal kinase （JNK） pathway inhibitor, SP600125, and the p38 pathway inhibitor, SB203580, to investigate the roles of these pathways in HIV-1 replication. We found that application of PD98059 results in a strong VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus and HIV-1NL4-3 virus inhibition activity. In addition, SB203580 and SP600125 also elicited marked VSV-G pseudotyped HIV-INL4-3 luciferase reporter virus inhibition activity but no HIV-1NL4-3 virus inhibition activity. We also found that SB203580 and SP600125 can enhance the HIV-1 inhibition activity of PD98059 when cells were treated with all three MAPK pathway inhibitors in combination. Finally, we show that HIV-1 virus inhibition activity of the MAPK pathway inhibitors was the result of the negative regulation of HIV-1 LTR promoter activity.
基金Project(51021004)supported by Innovative Research Groups of the National Natural Science Foundation of ChinaProject(NCET-11-0370)supported by Program for New Century Excellent Talents in Universities of China+1 种基金Project(40776055)supported by the National Natural Science Foundation of ChinaProject(1002)supported by State Key Laboratory of Ocean Engineering Foundation,China
文摘Buckling of submarine pipelines under thermal stress is one of the most important problems to be considered in pipeline design. And pipeline with initial imperfections will easily undergo failure due to global buckling under thermal stress and internal pressure. Therefore, it is vitally important to study the global buckling of the submarine pipeline with initial imperfections. On the basis of the characteristics of the initial imperfections, the global lateral buckling of submarine pipelines was analyzed. Based on the deduced analytical solutions for the global lateral buckling, effects of temperature difference and properties of foundation soil on pipeline buckling were analyzed. The results show that the snap buckling is predominantly governed by the amplitude value of initial imperfection; the triggering temperature difference of Mode I for pipelines with initial imperfections is higher than that of Mode I1; a pipeline with a larger friction coefficient is safer than that with a smaller one; pipelines with larger initial imperfections are safer than those with smaller ones.
