Objective:To observe effects of meshed relaxing short incisions (MRSI) on the content of endothelin(ET) in rat tension skin flap,and to investigate the mechanism of meshed relaxing short incisions (MRSI) on wound hea...Objective:To observe effects of meshed relaxing short incisions (MRSI) on the content of endothelin(ET) in rat tension skin flap,and to investigate the mechanism of meshed relaxing short incisions (MRSI) on wound healing process of tension skin flap.Method:We designed an experimental model to investigate the change of ET content caused by MRSI and observe the distribution of ET in rat skin flap tissue each period of wound healing.In the meantime,the immunohistochemistry and auto-image analysis were employed.Results:our results showed:(1) ET positive immunologic reaction granules involved in small vascular endothelial cell in rat skin flap tissue increased with prolonging of time within 3 days;(2)the content of ET in MRSI group were significant lower than that of hypertension group (P<0.05).Conclusion:The decrease of ET contents may be one of the causes that MRSI improves microcirculation of skin flap,and reduces edema and facilitates healing of skin flap.展开更多
Objective To observe effects of meshed relaxing short incisions on the level of superoxide dismutase and malondialdehyde in rat tension skin flap, and to investigate the mechanism of meshed relaxing short incisions (M...Objective To observe effects of meshed relaxing short incisions on the level of superoxide dismutase and malondialdehyde in rat tension skin flap, and to investigate the mechanism of meshed relaxing short incisions (MRSI) on wound healing process of tension skin flap. Method An experimental model was designed to investingate he changes of superoxide dismutase (SOD) and malondialdehyde (MDA) in rat shin flap tissue each period of wound healing (12h,24h,48h,72h after suture). In the meantime, the biochemical reaction were employed. Results Our results showed that SOD content in rat skin flap tissue from MRSI group were significant higher than that of hypertension group (P≤0.05), while MDA content was significantly lower (P≤0.05). Conclusion The decrease of MDA contents may be one of the causes that MRSI improves microcirculation of skin flap, and reduces edema and facilitates healing of skin flap.展开更多
文摘Objective:To observe effects of meshed relaxing short incisions (MRSI) on the content of endothelin(ET) in rat tension skin flap,and to investigate the mechanism of meshed relaxing short incisions (MRSI) on wound healing process of tension skin flap.Method:We designed an experimental model to investigate the change of ET content caused by MRSI and observe the distribution of ET in rat skin flap tissue each period of wound healing.In the meantime,the immunohistochemistry and auto-image analysis were employed.Results:our results showed:(1) ET positive immunologic reaction granules involved in small vascular endothelial cell in rat skin flap tissue increased with prolonging of time within 3 days;(2)the content of ET in MRSI group were significant lower than that of hypertension group (P<0.05).Conclusion:The decrease of ET contents may be one of the causes that MRSI improves microcirculation of skin flap,and reduces edema and facilitates healing of skin flap.
文摘Objective To observe effects of meshed relaxing short incisions on the level of superoxide dismutase and malondialdehyde in rat tension skin flap, and to investigate the mechanism of meshed relaxing short incisions (MRSI) on wound healing process of tension skin flap. Method An experimental model was designed to investingate he changes of superoxide dismutase (SOD) and malondialdehyde (MDA) in rat shin flap tissue each period of wound healing (12h,24h,48h,72h after suture). In the meantime, the biochemical reaction were employed. Results Our results showed that SOD content in rat skin flap tissue from MRSI group were significant higher than that of hypertension group (P≤0.05), while MDA content was significantly lower (P≤0.05). Conclusion The decrease of MDA contents may be one of the causes that MRSI improves microcirculation of skin flap, and reduces edema and facilitates healing of skin flap.
