A simple and sensitive analytical procedure for the determination of multi-component compounds in water samples was developed and optimized using the headspace solid-phase microextraction(HSSPME) coupled with gas chro...A simple and sensitive analytical procedure for the determination of multi-component compounds in water samples was developed and optimized using the headspace solid-phase microextraction(HSSPME) coupled with gas chromatography-mass spectrometry(GC-MS). Ten off-flavor compounds, including geosmin(GSM), 2-methylisoborneol(2-MIB), 2-isopropyl-3-methoxypyrazine(IPMP), 2-isobutyl-3-methoxypyrazine(IBMP), β-ionone, trans-2,cis-6-nonadienal(NDE), 2,3,4-trichloroanisole(2,3,4-TCA), 2,3,6-trichroloanisole(2,3,6-TCA), 2,4,6-trichloroanisole(2,4,6-TCA), and 2,4,6-tribromoanisole(2,4,6-TBA) were used as the target analytes. The optimization of extraction parameters including fibers types, extraction time, extraction temperature, stirring rate, sample volume, and ionic strength was carried out through the univariate approach. Ten off-flavor compounds were quantified within 50 min under the optimal conditions. Calibration curves with good linearity(r^2=0.990-0.998) were obtained in the range 1.0/2.0-100 ng/L, while the limits of detection for all compounds were lower than or close to the odor threshold concentration. Furthermore, the proposed method was applied to analyzing and determining the off-flavor compounds in real water samples from water-treatment plants.展开更多
This article presents, based on field survey and in the consulted literature, the main procedures for fixing of claddings with plates of rocks, adopted in the city of Sao Paulo for buildings and contemporary urban wor...This article presents, based on field survey and in the consulted literature, the main procedures for fixing of claddings with plates of rocks, adopted in the city of Sao Paulo for buildings and contemporary urban works. There was an increase in the quality of these claddings in the last decades due to several factors, such as the adoption of technological innovations in the extraction process of the rocky material and in the productive chain and, also, the use of most modem procedures for projects in which are incorporated new knowledge for better use of the rocky material and reduction of waste, due to greater demands of the market in the sector of omamental stones.展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.展开更多
A melissopalynological study carried out on 35 bee pollen samples from Romania （Transylvania） shows predominant species as Taraxacum officinale, Tilia sp., Ferbascum sp., Zea mays L., Onobrychis viciifolia Scop., Ge...A melissopalynological study carried out on 35 bee pollen samples from Romania （Transylvania） shows predominant species as Taraxacum officinale, Tilia sp., Ferbascum sp., Zea mays L., Onobrychis viciifolia Scop., Geranium sanguineum L., Filipendula ulmaria L., Cydonia oblonga L., Calluna vulgaris L. and Brassica sp.. This is the first time for the contribution of total phenolic （TP） and total carotenoid （TC） content to the modified oxygen radical absorbance capacity （ORACFL） values in 29 monofloral samples. Hydrophilic ORACFL （H-ORACFL） values ranged between 7.13-10.12 μmol TE/g and 6.94-22.46 μmol TE/g, whereas lipophilic ORACFL （L-ORACFL）values were between 2.19-7.79 μmol TE/g and 1.65-9.96 μmol TE/g, in multifloral and monofloral samples, respectively. It has proved the complex involvement of botanical origins on the antioxidant features with a specific occurrence. Some monofloral samples presented particular high antioxidant potential, such as Salix sp., Taraxacum officinale, Matricaria chamomilla, Cichorium intybus and the poorly studied Cirsium arvense （L.） Scop. and Scilla bifolia L.. New variables, such as soil characteristics, climates together with botanical origins, were introduced in a multivariate analysis of antioxidant data matrix, given a possible important involvement of all of them in affecting, not only the phytochemical composition, but thereafter the antioxidant capacity. All these data could be crucial to a new way of gathering, by beekeepers, depending on the market demand and the purposes for the product that has a potential for further therapeutic bioactivities investigations, and added value in the enrichment of certain products.展开更多
OBJECTIVE:To collect and screen for ethnopharmacological properties(antileishmanial,antibacterial and brine lethality assays) of medicinal plant Ocimum basilicum from Peshawar region(34.008 latitude and 71.57 altitude...OBJECTIVE:To collect and screen for ethnopharmacological properties(antileishmanial,antibacterial and brine lethality assays) of medicinal plant Ocimum basilicum from Peshawar region(34.008 latitude and 71.57 altitudes).METHODS:In the present study a general antileishmanial activity against Leishmania tropica strain was carried out.The antibacterial potential of the plant was performed against 06 gram positive and 06 gram negative bacteria.Brine shrimp cytotoxicity assay at different concentrations were investigated.RESULTS:The anti-promastigotes profile of the plant showed good antileishmanial activity exhibited LC_(50) value 21.67 μg/mL.The result for gram positive antibacterial activity revealed that the O.basilicum leaves extract possesses significant inhibitory activity at highest two concentrations ranging from20.66 ± 0.31 to 31.86 ± 0.80 for Clostridium perfringens type C and Bacillus subtitilis,respectively,as compared to the gentamycin(27.36 ± 0.55 and21.80 ± 0.72,respectively).For gram negative bacteria good activity was observed.A highest zone of inhibition was recorded for Pseudomonas aeroginosa(28.83 ± 0.28) atthe highest concentration(10 mg/mL).The LC_(50) value obtained for brine shrimp lethality assay was 91.56 μg/mL.CONCLUSION:The herb basil possesses effective cidal activities which make this plant a good candidate for the isolation of antiprotozoal and antibacterial compounds which may lead to the development of novel drug.展开更多
基金Project(21277175) supported by the National Natural Science Foundation of ChinaProject(JCYJ20120618164317119) supported by Shenzhen Special Fund for Development of Strategic Emerging,China
文摘A simple and sensitive analytical procedure for the determination of multi-component compounds in water samples was developed and optimized using the headspace solid-phase microextraction(HSSPME) coupled with gas chromatography-mass spectrometry(GC-MS). Ten off-flavor compounds, including geosmin(GSM), 2-methylisoborneol(2-MIB), 2-isopropyl-3-methoxypyrazine(IPMP), 2-isobutyl-3-methoxypyrazine(IBMP), β-ionone, trans-2,cis-6-nonadienal(NDE), 2,3,4-trichloroanisole(2,3,4-TCA), 2,3,6-trichroloanisole(2,3,6-TCA), 2,4,6-trichloroanisole(2,4,6-TCA), and 2,4,6-tribromoanisole(2,4,6-TBA) were used as the target analytes. The optimization of extraction parameters including fibers types, extraction time, extraction temperature, stirring rate, sample volume, and ionic strength was carried out through the univariate approach. Ten off-flavor compounds were quantified within 50 min under the optimal conditions. Calibration curves with good linearity(r^2=0.990-0.998) were obtained in the range 1.0/2.0-100 ng/L, while the limits of detection for all compounds were lower than or close to the odor threshold concentration. Furthermore, the proposed method was applied to analyzing and determining the off-flavor compounds in real water samples from water-treatment plants.
文摘This article presents, based on field survey and in the consulted literature, the main procedures for fixing of claddings with plates of rocks, adopted in the city of Sao Paulo for buildings and contemporary urban works. There was an increase in the quality of these claddings in the last decades due to several factors, such as the adoption of technological innovations in the extraction process of the rocky material and in the productive chain and, also, the use of most modem procedures for projects in which are incorporated new knowledge for better use of the rocky material and reduction of waste, due to greater demands of the market in the sector of omamental stones.
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.
文摘A melissopalynological study carried out on 35 bee pollen samples from Romania （Transylvania） shows predominant species as Taraxacum officinale, Tilia sp., Ferbascum sp., Zea mays L., Onobrychis viciifolia Scop., Geranium sanguineum L., Filipendula ulmaria L., Cydonia oblonga L., Calluna vulgaris L. and Brassica sp.. This is the first time for the contribution of total phenolic （TP） and total carotenoid （TC） content to the modified oxygen radical absorbance capacity （ORACFL） values in 29 monofloral samples. Hydrophilic ORACFL （H-ORACFL） values ranged between 7.13-10.12 μmol TE/g and 6.94-22.46 μmol TE/g, whereas lipophilic ORACFL （L-ORACFL）values were between 2.19-7.79 μmol TE/g and 1.65-9.96 μmol TE/g, in multifloral and monofloral samples, respectively. It has proved the complex involvement of botanical origins on the antioxidant features with a specific occurrence. Some monofloral samples presented particular high antioxidant potential, such as Salix sp., Taraxacum officinale, Matricaria chamomilla, Cichorium intybus and the poorly studied Cirsium arvense （L.） Scop. and Scilla bifolia L.. New variables, such as soil characteristics, climates together with botanical origins, were introduced in a multivariate analysis of antioxidant data matrix, given a possible important involvement of all of them in affecting, not only the phytochemical composition, but thereafter the antioxidant capacity. All these data could be crucial to a new way of gathering, by beekeepers, depending on the market demand and the purposes for the product that has a potential for further therapeutic bioactivities investigations, and added value in the enrichment of certain products.
文摘OBJECTIVE:To collect and screen for ethnopharmacological properties(antileishmanial,antibacterial and brine lethality assays) of medicinal plant Ocimum basilicum from Peshawar region(34.008 latitude and 71.57 altitudes).METHODS:In the present study a general antileishmanial activity against Leishmania tropica strain was carried out.The antibacterial potential of the plant was performed against 06 gram positive and 06 gram negative bacteria.Brine shrimp cytotoxicity assay at different concentrations were investigated.RESULTS:The anti-promastigotes profile of the plant showed good antileishmanial activity exhibited LC_(50) value 21.67 μg/mL.The result for gram positive antibacterial activity revealed that the O.basilicum leaves extract possesses significant inhibitory activity at highest two concentrations ranging from20.66 ± 0.31 to 31.86 ± 0.80 for Clostridium perfringens type C and Bacillus subtitilis,respectively,as compared to the gentamycin(27.36 ± 0.55 and21.80 ± 0.72,respectively).For gram negative bacteria good activity was observed.A highest zone of inhibition was recorded for Pseudomonas aeroginosa(28.83 ± 0.28) atthe highest concentration(10 mg/mL).The LC_(50) value obtained for brine shrimp lethality assay was 91.56 μg/mL.CONCLUSION:The herb basil possesses effective cidal activities which make this plant a good candidate for the isolation of antiprotozoal and antibacterial compounds which may lead to the development of novel drug.
