Objective To study the effect of the amniotic membrane on enhancing the proliferation ofcorneal epithelia and YAC-1 cell. Methods After the primary culture of the rabbit's corneal epithelia and YAC-1 cells, they w...Objective To study the effect of the amniotic membrane on enhancing the proliferation ofcorneal epithelia and YAC-1 cell. Methods After the primary culture of the rabbit's corneal epithelia and YAC-1 cells, they were seeded on the upper surface or stromal matrix side of amniotic membrane respectively. The proliferation results were observed by MTT test. Results The amniotic membrane was found significantly enhancing the proliferation of corneal epithelia on the d1 ,d3 , and d5 after culture. The proliferation rate was 28.93% ,23.32% ,23.41 % (P<0 .05) respectively, but the d7 proliferation rate was 20.72% (P> 0.05). On the dl , d3 , d7 after culture , the YAC-1 cells proliferation rate was 34 .87% ,36 .28% ,33 .86% (P< 0.01) respectively. Conclusion Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly. Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surface, special attention should be paid during amniotic membrane transplantation for treating ocular surface lesion resulted from epibulbar tumors.展开更多
OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burn...OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns. METHODS: The proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns. RESULTS: Corneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established. CONCLUSIONS: Ocular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.展开更多
文摘Objective To study the effect of the amniotic membrane on enhancing the proliferation ofcorneal epithelia and YAC-1 cell. Methods After the primary culture of the rabbit's corneal epithelia and YAC-1 cells, they were seeded on the upper surface or stromal matrix side of amniotic membrane respectively. The proliferation results were observed by MTT test. Results The amniotic membrane was found significantly enhancing the proliferation of corneal epithelia on the d1 ,d3 , and d5 after culture. The proliferation rate was 28.93% ,23.32% ,23.41 % (P<0 .05) respectively, but the d7 proliferation rate was 20.72% (P> 0.05). On the dl , d3 , d7 after culture , the YAC-1 cells proliferation rate was 34 .87% ,36 .28% ,33 .86% (P< 0.01) respectively. Conclusion Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly. Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surface, special attention should be paid during amniotic membrane transplantation for treating ocular surface lesion resulted from epibulbar tumors.
文摘OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns. METHODS: The proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns. RESULTS: Corneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established. CONCLUSIONS: Ocular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.
