The optimal conditions of the conversion of 16α methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate to 16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione by the mixed cultures of Arthrobacter sp 8...The optimal conditions of the conversion of 16α methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate to 16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione by the mixed cultures of Arthrobacter sp 86 and Metarhizium sp 88 were studied.2%～4%( V/V )N,N dimethylformamide in the medium was used to dissolve the substrate.It could increase the conversion ability.Adding the mycelium of Metarhizium into the dehydrogenation reaction mixture during 0～28 hours had no influence on the conversion rate.The optimal concentration of mycelium was around 75 mg/mL wet weight.0.04%～0.06% CoCl 2·6H 2O inhibited the degradation of steroid nucleus so that the product was greatly accumulated.Under such condition the yeild of mixed microbial conversion was 67%.展开更多
文摘The optimal conditions of the conversion of 16α methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate to 16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione by the mixed cultures of Arthrobacter sp 86 and Metarhizium sp 88 were studied.2%～4%( V/V )N,N dimethylformamide in the medium was used to dissolve the substrate.It could increase the conversion ability.Adding the mycelium of Metarhizium into the dehydrogenation reaction mixture during 0～28 hours had no influence on the conversion rate.The optimal concentration of mycelium was around 75 mg/mL wet weight.0.04%～0.06% CoCl 2·6H 2O inhibited the degradation of steroid nucleus so that the product was greatly accumulated.Under such condition the yeild of mixed microbial conversion was 67%.
