肝龙胶囊和美洲大蠊脱脂膏对正常大鼠胆汁分泌的影响

目的:研究肝龙胶囊和美洲大蠊脱脂膏对正常大鼠胆汁分泌的影响。方法:取80只大鼠随机分组,各组均按20 m L/kg进行灌胃给药,1次/d,连续给药7 d。采用大鼠胆汁流量实验、胆汁成分分析观察受试药的利胆作用。结果:肝龙胶囊无利胆作用,对胆汁成分无影响;而美洲大蠊脱脂膏具有利胆作用,其胆汁流量与对照组比较差异有统计学意义(P<0.05),使胆汁中TBL、DBL、IBL的含量降低(P<0.05)。结论:肝龙胶囊对正常大鼠无利胆作用;美洲大蠊脱脂膏则有利胆作用。

美洲大蠊脱脂膏中总糖和还原糖含量的测定

目的:采用不同方法测定美洲大蠊脱脂膏中总糖含量,优选出测定美洲大蠊脱脂膏中总糖含量的方法,并建立其还原糖含量测定方法。方法:以葡萄糖为标准品,分别采用苯酚-硫酸法和硫酸-蒽酮法测定总糖含量,采用二硝基水杨酸法测定还原糖含量。结果:苯酚-硫酸法标准品浓度在0.004～0.100 mg/m L范围内呈良好的线性关系,平均加样回收率为98.7%,RSD为2.0%,R^2=0.9996。硫酸-蒽酮法标准品浓度在0.02～0.20 mg/m L范围内有良好的线性关系,平均加样回收率为97.7%,RSD为2.6%,R^2=0.9997。二硝基水杨酸法标准品浓度在0.004～0.040 mg/m L范围内呈良好的线性关系,平均加样回收率为96.8%,RSD为1.6%,R^2=0.9996。结论:苯酚-硫酸法可用于脱脂膏中总糖含量的测定,二硝基水杨酸法可用于脱脂膏中还原糖含量的测定。

美洲大蠊脱脂膏对小鼠免疫功能的影响

目的:研究美洲大蠊脱脂膏(PAE)对正常小鼠和环磷酰胺造成的免疫低下小鼠免疫功能的影响。方法:对C57BL/6小鼠腹腔注射环磷酰胺制备免疫低下模型,PAE高、中、低剂量(4 g/kg,2 g/kg,1 g/kg)灌胃15 d,测定各组的T、B淋巴细胞的增殖能力,脏器质量和脏器指数,外周血细胞数目,骨髓有核细胞计数等。结果:PAE可以促进脾淋巴细胞的增殖功能,提高免疫低下小鼠的脏器质量和脏器指数,改善其外周血象,以及增加骨髓有核细胞的数目从而提高其免疫功能。结论:PAE可以改善免疫低下小鼠的免疫功能,而对正常小鼠的免疫功能几乎没有作用。

Comparison between Decolorization Effects of Activated Carbon and Macroporous Resin on Periplaneta americana L. Skimmed Cream

[Objective] The aim of this study was to investigate the decolorization ef- fects of activated carbon and macroporous resin on Periplaneta americana L. skimmed cream and compare the advantages and disadvantages of the two decolorization technologies. [Method] Periplaneta americana L. skimmed cream was decolored with activated carbon and macroporous resin, and freeze-dried to collect solid decolorization products. By investigating the yield, decolorization rate, protein retention rate and decolorization operation process, the advantages and disadvantages of the two decolorization technologies were compared. [Result] Both activated carbon and macroporous resin can be used for decolorization. To be specific, macroporous resin-decolorization is superior in the yield and protein retention rate, while activated carbon-decolorization is superior in decolorization rate and decolorization operation process. [Conclusion] Macroporous resin-decolorization can be used if protein is the main ingredient required in the experiment, while activated carbon-decolorization can be used if protein is not the main ingredient required.