Effects of dopamine injection on the hemocyte count, phenoloxidase activity, serine proteinase activity, proteinase in-hibitor activity and α2-macroglobulin-like activity in L. vannamei were studied. Results showed t...Effects of dopamine injection on the hemocyte count, phenoloxidase activity, serine proteinase activity, proteinase in-hibitor activity and α2-macroglobulin-like activity in L. vannamei were studied. Results showed that dopamine injection resulted in a significant effect on the parameters measured (P < 0.05), while no significant difference was observed in the control group (0.85% NaCl). In the experimental groups,the hemocyte count reached the minimum in 3 h;granular and semi-granular cells became stable after 12 h and hyaline cells and the total hemocyte count became stable after 18 h. Phenoloxidase activity reached the minimum in 6 h, and then became stable after 9 h. Serine protease activity and proteinase inhibitor activity reached the minimum in 3 h, and α2-macro-globulin-like activity reached the maximum in 3 h,and all the three parameters became stable after 12 h. The results suggest that the activating mechanisms of the proPO system triggered by dopamine are different from those triggered by invasive agents or sponta-neously activated under a normal physical condition.展开更多
In this study,we investigated the effects of low salinity(26 and 21) on the prophenoloxidase(proPO) system and related defence parameters in the shrimp Litopenaeus vannamei.The results showed that low salinity induced...In this study,we investigated the effects of low salinity(26 and 21) on the prophenoloxidase(proPO) system and related defence parameters in the shrimp Litopenaeus vannamei.The results showed that low salinity induced a significant increase of dopamine(DA) concentration in haemolymph at 6 h of the experiment;on the other hand,total haemocyte count(THC),differential haemocyte count(DHC) and PO activity decreased over time to the lowest level at 24 h and remained low thereafter.Serine Protease(SP) and Proteinase Inhibitor(PI) activity in the two lower salinity treatments decreased to the lowest level at 12 and 24 h,respectively,and both recovered to the control level at 72 h.In contrast,α2-macroglobulin(α2M) activity in the two lower salinity treatments peaked at 24 h and then decreased to the control level at 72 h.Therefore,it may be concluded that stress-induced DA plays an important temporary role in neurotransmission and causes immune response in L.vannamei in adapting to salinity changes.展开更多
Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of...Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom(mode-C),greenhouse tanks with cement bottom(mode-G) and outdoor tanks with mud-substrate(mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C(P < 0.05).In contrast to the other two treatments,mode-M had stable pH after 50 d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C(P < 0.05).Despite lack of differences in the final shrimp weight among different treatments(P > 0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate(P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments(P < 0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system of L.vannamei.展开更多
基金supported by the Program for New century excellent talents in university(NCET-06-0597)the program transformation and expansion of achievement of agricultural science and technology in Tianjin,China(0604020)
文摘Effects of dopamine injection on the hemocyte count, phenoloxidase activity, serine proteinase activity, proteinase in-hibitor activity and α2-macroglobulin-like activity in L. vannamei were studied. Results showed that dopamine injection resulted in a significant effect on the parameters measured (P < 0.05), while no significant difference was observed in the control group (0.85% NaCl). In the experimental groups,the hemocyte count reached the minimum in 3 h;granular and semi-granular cells became stable after 12 h and hyaline cells and the total hemocyte count became stable after 18 h. Phenoloxidase activity reached the minimum in 6 h, and then became stable after 9 h. Serine protease activity and proteinase inhibitor activity reached the minimum in 3 h, and α2-macro-globulin-like activity reached the maximum in 3 h,and all the three parameters became stable after 12 h. The results suggest that the activating mechanisms of the proPO system triggered by dopamine are different from those triggered by invasive agents or sponta-neously activated under a normal physical condition.
基金supported by the Program for New Century Excellent Talents in University (NCET-06-0597)the project of Transformation and Expansion of Achievement of Agricultural Science and Technology in Tianjin, China (0604020)
文摘In this study,we investigated the effects of low salinity(26 and 21) on the prophenoloxidase(proPO) system and related defence parameters in the shrimp Litopenaeus vannamei.The results showed that low salinity induced a significant increase of dopamine(DA) concentration in haemolymph at 6 h of the experiment;on the other hand,total haemocyte count(THC),differential haemocyte count(DHC) and PO activity decreased over time to the lowest level at 24 h and remained low thereafter.Serine Protease(SP) and Proteinase Inhibitor(PI) activity in the two lower salinity treatments decreased to the lowest level at 12 and 24 h,respectively,and both recovered to the control level at 72 h.In contrast,α2-macroglobulin(α2M) activity in the two lower salinity treatments peaked at 24 h and then decreased to the control level at 72 h.Therefore,it may be concluded that stress-induced DA plays an important temporary role in neurotransmission and causes immune response in L.vannamei in adapting to salinity changes.
基金supported by the National Key Technologies R&D Program of China(2007BAD43B06)the Ocean University of China,R&D Institute of Zhejiang,and Zhejiang Ocean University
文摘Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom(mode-C),greenhouse tanks with cement bottom(mode-G) and outdoor tanks with mud-substrate(mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C(P < 0.05).In contrast to the other two treatments,mode-M had stable pH after 50 d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C(P < 0.05).Despite lack of differences in the final shrimp weight among different treatments(P > 0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate(P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments(P < 0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system of L.vannamei.
