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湖北省武汉市某门诊猫传染性腹膜炎病例统计分析 被引量:19
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作者 唐小娟 王姗 +4 位作者 尹伊娅 胡长敏 丁明星 陈建国 韩丽 《中国动物检疫》 CAS 2019年第4期22-25,共4页
为了解湖北省武汉市猫传染性腹膜炎的流行情况,以华中农业大学动物医院的门诊临床病例为研究对象,运用猫传染性腹膜炎抗原快速诊断试纸条和RT-PCR检测技术,对2017年11月至2018年9月的1 036例临床病例进行猫传染性腹膜炎检测,并对病例的... 为了解湖北省武汉市猫传染性腹膜炎的流行情况,以华中农业大学动物医院的门诊临床病例为研究对象,运用猫传染性腹膜炎抗原快速诊断试纸条和RT-PCR检测技术,对2017年11月至2018年9月的1 036例临床病例进行猫传染性腹膜炎检测,并对病例的发病情况和特点进行统计分析。共检出猫传染性腹膜炎阳性病例61例,检出率为5.9%。从时间分布看,2018年1月的检出率最高,占13.4%,其余月份的检出率为6.0%~8.0%。从群间分布看,英国短毛猫、田园猫、美国短毛猫的患病比例较高,占总阳性病例的76.7%;雄性猫(70.0%)患病比例高于雌性猫(30.0%),1岁以下幼猫的阳性占比为72.1%。本研究对于了解猫传染性腹膜炎流行发病特点以及该病的临床防治具有一定参考价值。 展开更多
关键词 猫传染性腹膜炎 流行病学 分布 分布 群间分布 RT-PCR
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三类禽源碳青霉烯类耐药大肠杆菌流行病学调查及全基因组测序分析 被引量:5
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作者 李翠函 曲志娜 +12 位作者 高玉斌 李彦 王娟 段笑笑 王琳 张喜悦 赵格 黄秀梅 赵建梅 张青青 王君玮 黄保续 刘俊辉 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第4期1653-1662,共10页
【目的】研究不同禽源碳青霉烯类耐药大肠杆菌(carbapenem-resistant Escherichia coli,CREC)的群间分布、耐药特征及菌株间亲缘关系,为阻断CREC的潜在危害提供技术支持。【方法】在胶东地区采集肉鸡、蛋鸡、水禽三类家禽泄殖腔拭子1131... 【目的】研究不同禽源碳青霉烯类耐药大肠杆菌(carbapenem-resistant Escherichia coli,CREC)的群间分布、耐药特征及菌株间亲缘关系,为阻断CREC的潜在危害提供技术支持。【方法】在胶东地区采集肉鸡、蛋鸡、水禽三类家禽泄殖腔拭子1131份,利用选择性分离培养、质谱鉴定、PCR、微量肉汤稀释法、多位点序列分型(MLST)及全基因组测序(WGS)等方法进行CREC菌株鉴定与分析。【结果】共分离出364株bla NDM基因阳性的CREC菌株,分离率为32.18%,阳性场总体占比为76.32%,肉鸡场和个体阳性率均最高,分别为93.33%和55.56%。三类禽源菌株均多重耐药,83.65%菌株对8类及以上药物同时耐药,对多数测试药物耐药率在80%以上。肉鸡多重耐药问题最严重,水禽次之,蛋鸡相对较轻。45株全基因组测序的CREC菌株携带12类52种耐药基因,4种bla NDM变异体,以bla NDM-5(77.78%)为主,三类家禽对同类耐药基因的检出率存在差异。共检出46种ST型,多样性比为44.23%。三类家禽CREC菌株间单核苷酸多态性(SNPs)差异位点为82~71307个,且携带的优势型不同。【结论】携带bla NDM的CREC在不同家禽养殖场尤其是肉鸡场广泛存在,以bla NDM-5亚型最为常见,对多种抗菌药普遍耐药,且携带大量耐药基因,以质粒传播为主。CREC菌株在三类家禽中呈多样化分布,且多数菌株间亲缘关系相差较远。提示应针对不同动物群体,加强监测和影响因素研究,以降低CREC的潜在风险。 展开更多
关键词 家禽 碳青霉烯类耐药大肠杆菌(CREC) 群间分布 多位点序列分型(MLST) 全基因组测序(WGS)
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Horizontal Distribution and Annual Variations of Fecal Coliform Bacteria in Luoyuan Bay 被引量:2
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作者 蔡雷鸣 《Marine Science Bulletin》 CAS 2010年第1期48-58,共11页
Fecal Coliform Bacteria (FCB) of marine waters was monitored in Luoyuan Bay from January, 2003 to December, 2005. The results showed that number of FCB in marine water samples ranged from no detection ( 〈2 cfu/100... Fecal Coliform Bacteria (FCB) of marine waters was monitored in Luoyuan Bay from January, 2003 to December, 2005. The results showed that number of FCB in marine water samples ranged from no detection ( 〈2 cfu/100 mL) to 540 cfu/100 mL in Luoyuan Bay. Values of FCB during August to October were significantly higher than those during January to April during three year period. Monthly changes of FCB values at each year period were mainly due to monthly precipitation. In addition, compared with FCB values in difference sample sites, values of FCB in Bay-heed were significantly higher than those in middle of bay and Bay-mouth, Luoyuan Bay. However, values of FCB in Bay-mouth were significantly less than those in middle of bay. The ratios to the par of FCB in 2003, 2004, and 2005 years were 100%, 98.0%, and 97.9%, respectively. Therefore, we considered that the pollution of FCB of surface marine water in Luoyuan Bay was not serious. 展开更多
关键词 Luoyuan Bay fecal coliform bacteria horizontal distribution annual variations
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Field Modeling Method for Identifying Urban Sphere of Influence:A Case Study on Central China 被引量:7
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作者 DENG Yu LIU Shenghe +2 位作者 WANG Li MA Hanqing WANG Jianghao 《Chinese Geographical Science》 SCIE CSCD 2010年第4期353-362,共10页
With rapid development of urbanization and regional interaction and interdependence, regional urban agglomeration planning becomes more and more important in China, in order to promote integrated development of variou... With rapid development of urbanization and regional interaction and interdependence, regional urban agglomeration planning becomes more and more important in China, in order to promote integrated development of various cities with close interrelationship. However, it is still arguable academically on how to define the boundary or which cities to be included for the urban agglomeration of a region. This paper aims to shed lights on how to identify urban spheres of influence scientifically by introducing field modeling method and by practicing a case study on 168 cities in Central China. In our field modeling method, the influence intensities of cities were measured by a compre-ensive index and urban spheres of influence were represented spatially by field intensity. Then, their classification and spatial distribution characteristics of study area in 2007 were identified and explored by using GIS and statistical methods. The result showed that: 1) Wuhan is the absolute dominant city in Central China; 2) the provincial capital cities dominate their own provinces and there are no other lower grade agglomeration centers; and 3) the basic types of organization form of urban sphere of influence are single-polar type, agglomeration type, close-related group type and loose-related group type. 展开更多
关键词 Central China urban sphere of influence urban influence index field intensity urban influence intensity
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Spatial and Vertical Distribution of Phytoplankton During a Winter Cruise in the South of the Kerkennah Islands in the Gulf of Gabes, Tunisia
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作者 Geurmazi Wassim Drira Zaher Elloumi Jannet Bourn Abderrahmen Ayadi Habib 《Journal of Life Sciences》 2010年第7期22-29,共8页
Spatial and vertical distribution of phytoplankton community were examined in the coast of the south of the Kerkennah Islands at three different depths 0, 2 and 6 m during the winter 2009 at 6 sampling stations. A tot... Spatial and vertical distribution of phytoplankton community were examined in the coast of the south of the Kerkennah Islands at three different depths 0, 2 and 6 m during the winter 2009 at 6 sampling stations. A total of 49 taxa belonging to five phytoplankton groups were identified (H'=1.7 to 3.5 bits'cell^-1). Dinoflagellates and Diatoms were the most abundant phytoplankton groups which reached 27 and 71% of total abundance, respectively. The spatial distribution of the phytoplankton abundance showed a significant variation between the stations (P 〈 0.001). While the vertical distribution of total phytoplankton showed a clear pattem, ANOVA test showed only an insignificant variation between the different depths at each station (P 〉 0.01). On the whole, the maximum abundance of phytoplankton was recorded at the depths of 2 and 6 m (6 ×10^3 ind..Ll). This might be due to the decrease of the nutrient concentrations in deeper water specially those of phosphate. 展开更多
关键词 Kerkennah Islands PHYTOPLANKTON spatial and vertical distribution environmental factors.
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Research on the Activity Characteristics of Small Earthquake Swarms in the Muli Area of Sichuan Province
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作者 Cai Yichuan Dai Shigui Wei Yaling 《Earthquake Research in China》 CSCD 2016年第2期215-223,共9页
In this article,we relocated the seismic source location of the earthquakes in the Muli area of Sichuan,inverted the focal mechanism of the larger earthquakes and analyzed the relationship between the water level of t... In this article,we relocated the seismic source location of the earthquakes in the Muli area of Sichuan,inverted the focal mechanism of the larger earthquakes and analyzed the relationship between the water level of the Jinping reservoir and the frequency of the earthquake swarm. The results show that:( 1) The epicenters of the relocated small earthquake swarms are distributed in a seismic zone,and the earthquake focal depths were in the range of 0- 12 km.( 2) By analyzing the earthquake swarm spatial distribution,we found that the swarms were generated by one branch fault on the west of Xiaojinhe fault.( 3) The focal mechanism of the three earthquakes with magnitude greater than 4. 0 is significantly different,with the shallow source thrust events affected by vertical stress,and the strike-slip events are related to regional stress tectonic activity. 展开更多
关键词 Earthquake swarm activities Seismic zone Double difference locationmethod Focal mechanism
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Composition and Spatial Distribution of Soil Mesofauna Along an Elevation Gradient on the North Slope of the Changbai Mountains, China 被引量:7
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作者 JIANG Yunfeng YIN Xiuqin WANG Fubin 《Pedosphere》 SCIE CAS CSCD 2015年第6期811-824,共14页
The Changbai Mountains, located in northeastern China, show clear vertical zonation of vegetation types. Six different habitats,namely Pinus koraiensis mixed broad-leaved forest, Pinus koraiensis-Picea forest, spruce-... The Changbai Mountains, located in northeastern China, show clear vertical zonation of vegetation types. Six different habitats,namely Pinus koraiensis mixed broad-leaved forest, Pinus koraiensis-Picea forest, spruce-fir forest, Betula ermanii forest, alpine meadow and alpine semi-desert, at elevations ranging from 780 to 2 480 m, covering almost all ecosystems on the north slope of the Changbai Mountains, were investigated to determine: i) whether or not the community composition of soil mesofauna varied significantly at different elevations; ii) if different soil mesofauna groups would respond differently to elevation and iii) which factors influenced the spatial distribution of soil mesofauna along elevation. Soil mesofauna were collected from each habitat in spring(May),summer(July) and autumn(September) of 2009. The soil mesofauna communities were comprised of at least 44 groups and were dominated by Acari and Collembola, followed by Coleoptera, Diptera larvae and Enchytraeidae. The composition, diversity and abundance of soil mesofauna varied among the six habitats. Meanwhile, significant seasonal variations were observed in the composition,abundance and diversity of the soil mesofauna in each habitat. The taxonomic richness and Shannon index were affected by elevation and soil properties, while the abundance was only significantly affected by soil properties. With regard to taxa, the habitats and seasons had significant effects on almost all the abundances of the major taxonomic groups. The abundance of more taxonomic groups was significantly influenced by the soil properties, while those of Geophilomorpha, Araneae and other taxa were affected by elevation.It is concluded that the composition and spatial distribution of the soil mesofauna varied along the elevation gradient on the north slope of the Changbai Mountains, which might be largely related to the variations of the plant community, soil properties and climate change resulting from the elevation gradient. 展开更多
关键词 ABUNDANCE community composition diversity environmental factors taxonomic group
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