According to the TCM theory that the kidney is in charge of the bone, the authors carried out a study on the effect of acupuncture on bone mineral density in 40 postmenopausal women with osteoporosis treated by th... According to the TCM theory that the kidney is in charge of the bone, the authors carried out a study on the effect of acupuncture on bone mineral density in 40 postmenopausal women with osteoporosis treated by the method of reinforcing the kidney to strengthen the bone, with satisfactory therapeutic results reported as follows.……展开更多
Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Method...Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Methods.PTH gene polymorphism was detected in270subjects by polymerase chain reaction(PCR)and PCR /restriction fragment length polymorphism(PCR /RFLP).The digestion products of restriction enzyme Bst B1were separated on1%agarose gels.PTH genotypes were confirmed by DNA sequences analysis.BMD was measured by dual-energy X-ray absorptiometry(DEXA,DPX -L ,Lunar).Results.Genotype frequencies of BB,Bb,bb were73.7%,25.9%and0.4%respectively in Beijing adults(P<0.01),67.1%,32.2%and0.7%in postmenopausal women,which was different from Japanese wom-en.We statistically compared bone mineral density at the lumbar2-4and proximal femur between BB and Bb genotypes.No obvious association was found between the BMD and PTH genotypes in Beijing women(P>0.05).Conclusion.PTH gene polymorphism is not associated with BMD in Chinese women.The further research to explore the genetic risk factors of osteoporosis should be committed.展开更多
文摘 According to the TCM theory that the kidney is in charge of the bone, the authors carried out a study on the effect of acupuncture on bone mineral density in 40 postmenopausal women with osteoporosis treated by the method of reinforcing the kidney to strengthen the bone, with satisfactory therapeutic results reported as follows.……
文摘Objective.To investigate the distribution frequency of parathyroid hormone(PTH)gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density(BMD).Methods.PTH gene polymorphism was detected in270subjects by polymerase chain reaction(PCR)and PCR /restriction fragment length polymorphism(PCR /RFLP).The digestion products of restriction enzyme Bst B1were separated on1%agarose gels.PTH genotypes were confirmed by DNA sequences analysis.BMD was measured by dual-energy X-ray absorptiometry(DEXA,DPX -L ,Lunar).Results.Genotype frequencies of BB,Bb,bb were73.7%,25.9%and0.4%respectively in Beijing adults(P<0.01),67.1%,32.2%and0.7%in postmenopausal women,which was different from Japanese wom-en.We statistically compared bone mineral density at the lumbar2-4and proximal femur between BB and Bb genotypes.No obvious association was found between the BMD and PTH genotypes in Beijing women(P>0.05).Conclusion.PTH gene polymorphism is not associated with BMD in Chinese women.The further research to explore the genetic risk factors of osteoporosis should be committed.
