OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necro...OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necrosis factor （TNF）-a, and interleu- kin （IL）-4, eosinophil counts, as well as expression of matrix metalloproteinase （MMP）-9, tissue inhibi- tor of metalloproteinase （TIMP）-I in the lung tissues of asthmatic rats. METHODS： Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups： normal control （NC）, asthma model, Guilong Kechuanning （GK） group, as well as high, intermediate-, and lowdose XP groups. Rats were sensitized with ovalbu- min （OVA） to trigger asthma. Serum concentrations of ECP, TNF-a and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method, mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative poly- merase chain reaction. RESULTS： Compared with the asthma-model group, serum concentrations of ECP, TNF-a, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9：TIMP-1 in lung tissue were significantly lower （P 〈 0.01）. CONCLUSION： XP can reduce TNF-a secretion, sup- press the infiltration/activation of eosinophils, re- duce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodel- ing of the airways.展开更多
基金Supported by Natural Science Foundation of China:Intervention Mechanism of the Method of Tonifying Qi And Promoting Blood Circulation and Resolving Phlem on Airway Remodeling after COPD(No.81473675)Based on p38MAP Signal Pathway Yiqi Huoxue Huatan Method was Explored the Mechanism Study of Bronchial Asthma Airway Remodeling(No.81774301)Excellent Young Talent Fund Project of Colleges and Universities in Anhui Province:Study on the Mechanism of Airway Remodeling of Asthma Model In Rats With Xiaochuanping Powder(No.2011SQRL093)
文摘OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necrosis factor （TNF）-a, and interleu- kin （IL）-4, eosinophil counts, as well as expression of matrix metalloproteinase （MMP）-9, tissue inhibi- tor of metalloproteinase （TIMP）-I in the lung tissues of asthmatic rats. METHODS： Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups： normal control （NC）, asthma model, Guilong Kechuanning （GK） group, as well as high, intermediate-, and lowdose XP groups. Rats were sensitized with ovalbu- min （OVA） to trigger asthma. Serum concentrations of ECP, TNF-a and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method, mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative poly- merase chain reaction. RESULTS： Compared with the asthma-model group, serum concentrations of ECP, TNF-a, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9：TIMP-1 in lung tissue were significantly lower （P 〈 0.01）. CONCLUSION： XP can reduce TNF-a secretion, sup- press the infiltration/activation of eosinophils, re- duce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodel- ing of the airways.
