Autophagic and proteasomal proteolysis are two major pathways for degradation of cellular constituents. Current models suggest that autophagy is responsible for the nonselective bulk degradation of long-lived proteins...Autophagic and proteasomal proteolysis are two major pathways for degradation of cellular constituents. Current models suggest that autophagy is responsible for the nonselective bulk degradation of long-lived proteins and organelles while the proteasome specifically degrades short-lived proteins including misfolded proteins caused by the absence of Hsp90 function. Here, we show that the IκB kinase （IKK）, an essential activator of NF-κB, is selectively degraded by autophagy when Hsp90 is inhibited by geldanamycin （GA）, a specific Hsp90 inhibitor showing highly effective anti-tumor activity. We find that in this case inactivation of ubiquitination or proteasome fails to block IKK degradation. However, inhibition of autophagy by an autophagy inhibitor or knockout of Atg5, a key component of the autophagy pathway, significantly rescues IKK from GA-induced degradation. These findings provide the first evidence that an Hsp90 client may be degraded by a mechanism different from the proteasome pathway and establish a molecular link among Hsp90, NF-κB and autophagy展开更多
The aim of this study is to identify the variables affecting land value. Examined land was selected from the village in Vientiane capital city Laos. Data was collected from 100 villages in center of Vientiane capital ...The aim of this study is to identify the variables affecting land value. Examined land was selected from the village in Vientiane capital city Laos. Data was collected from 100 villages in center of Vientiane capital city by using survey methods. A hedonic price analysis was conducted to determine the marginal return to different land characteristics using an econometric model corrected for correlation. Parcel characteristics such as distance to public park, village income, distance of population, number of the school within 500 meters buffer, distance to the temple, distance to the major market and distance to the business center （CBD）. Arc GIS 9.2 was applied to calculate the distance of the factors, after that SPSS 15.0 was used to calculate the land price characteristic based on hedonic price model. The results showed that the distance to the center of population was the main factor influencing to the land price, and followed by school and village income. Map of the land price before and after hedonic price analysis were produced. The land price valuation approaches based on hedonic price model for Vientiane capital city were developed and land price map were predicted. Hedonic price model and GIS were very useful for this research, and finally the policy of the land valuation based on GIS was developed.展开更多
文摘Autophagic and proteasomal proteolysis are two major pathways for degradation of cellular constituents. Current models suggest that autophagy is responsible for the nonselective bulk degradation of long-lived proteins and organelles while the proteasome specifically degrades short-lived proteins including misfolded proteins caused by the absence of Hsp90 function. Here, we show that the IκB kinase （IKK）, an essential activator of NF-κB, is selectively degraded by autophagy when Hsp90 is inhibited by geldanamycin （GA）, a specific Hsp90 inhibitor showing highly effective anti-tumor activity. We find that in this case inactivation of ubiquitination or proteasome fails to block IKK degradation. However, inhibition of autophagy by an autophagy inhibitor or knockout of Atg5, a key component of the autophagy pathway, significantly rescues IKK from GA-induced degradation. These findings provide the first evidence that an Hsp90 client may be degraded by a mechanism different from the proteasome pathway and establish a molecular link among Hsp90, NF-κB and autophagy
文摘The aim of this study is to identify the variables affecting land value. Examined land was selected from the village in Vientiane capital city Laos. Data was collected from 100 villages in center of Vientiane capital city by using survey methods. A hedonic price analysis was conducted to determine the marginal return to different land characteristics using an econometric model corrected for correlation. Parcel characteristics such as distance to public park, village income, distance of population, number of the school within 500 meters buffer, distance to the temple, distance to the major market and distance to the business center （CBD）. Arc GIS 9.2 was applied to calculate the distance of the factors, after that SPSS 15.0 was used to calculate the land price characteristic based on hedonic price model. The results showed that the distance to the center of population was the main factor influencing to the land price, and followed by school and village income. Map of the land price before and after hedonic price analysis were produced. The land price valuation approaches based on hedonic price model for Vientiane capital city were developed and land price map were predicted. Hedonic price model and GIS were very useful for this research, and finally the policy of the land valuation based on GIS was developed.
