目的:建立老范志万应神曲中总黄酮和多糖含量测定方法,并分析其不同发酵阶段中总黄酮和多糖含量变化规律,为老范志万应神曲质量控制方法研究提供参考。方法:采用紫外可见分光光度法,以芦丁为对照品,用NaNO_(2)-Al(NO_(3))_(3)-NaOH显色...目的:建立老范志万应神曲中总黄酮和多糖含量测定方法,并分析其不同发酵阶段中总黄酮和多糖含量变化规律,为老范志万应神曲质量控制方法研究提供参考。方法:采用紫外可见分光光度法,以芦丁为对照品,用NaNO_(2)-Al(NO_(3))_(3)-NaOH显色测定总黄酮含量;以葡萄糖为对照品,用苯酚-浓硫酸法显色测定多糖含量;通过单因素考察优化总黄酮和多糖的提取工艺和显色条件。结果:总黄酮的最佳提取工艺:90%乙醇,料液比为1∶30(g/mL),30 min,回流提取2次;测定总黄酮的最佳显色条件:1.0 mL 5%NaNO_(2),2.0 mL 10%Al(NO_(3))_(3),8.0 mL 8%NaOH;多糖最佳提取工艺:料液比1∶30(g/mL),20 min,回流提取2次;测定多糖的最佳显色条件:热水浴20 min,冷水浴15 min;以最佳提取工艺提取发酵48 h样品,测定样品总黄酮含量为2.4%,多糖含量为5.75%;方法学考察结果显示,新建总黄酮和多糖含量测定方法稳定,可行;样品的总黄酮和多糖含量在发酵48 h均达到峰值。结论:研究建立老范志万应神曲总黄酮和多糖含量测定方法;发酵过程中总黄酮和多糖的变化情况从侧面印证了老范志万应神曲传统炮制工艺的可行性和合理性。展开更多
Heart failure （HF） is a major public health problem worldwide entailing high morbidity and mortality as well as high costs. This chronic syndrome associates with a low functional status and quality of life. Most pa...Heart failure （HF） is a major public health problem worldwide entailing high morbidity and mortality as well as high costs. This chronic syndrome associates with a low functional status and quality of life. Most patients with HF are elderly, constituting up to 80% of patients suffering from this disease with both incidence and prevalence of the condition increasing with age. This is due to the progressive aging of the population as well as improved and better survival after cardiac insults, such as myocardial infarction,展开更多
The density of asphalt was measured with the pycnometer and densitometer. Creep properties of the asphalt were investigated with the bending beam rheometer at temperatures ranging from 0 ~C to -36 ~C. The asphalt dens...The density of asphalt was measured with the pycnometer and densitometer. Creep properties of the asphalt were investigated with the bending beam rheometer at temperatures ranging from 0 ~C to -36 ~C. The asphalt density data used to correlate with the creep properties were calculated from the regression equation of density and temperature. The asphalt sample used to determine the creep property was aged by the standard RTFOT test and the PAV test. The test results showed that the asphalt density had a linear relationship with temperature changes. The logarithm of the creep stiffness and the slope of the logarithm of the stiffness at 60 seconds all demonstrated a linear relationship with the density, and the regression coefficient of these data was around 0.99. The creep stiffness and the slope of the creep stiffness can be calculated from the asphalt density at the same temperature.展开更多
文摘目的:建立老范志万应神曲中总黄酮和多糖含量测定方法,并分析其不同发酵阶段中总黄酮和多糖含量变化规律,为老范志万应神曲质量控制方法研究提供参考。方法:采用紫外可见分光光度法,以芦丁为对照品,用NaNO_(2)-Al(NO_(3))_(3)-NaOH显色测定总黄酮含量;以葡萄糖为对照品,用苯酚-浓硫酸法显色测定多糖含量;通过单因素考察优化总黄酮和多糖的提取工艺和显色条件。结果:总黄酮的最佳提取工艺:90%乙醇,料液比为1∶30(g/mL),30 min,回流提取2次;测定总黄酮的最佳显色条件:1.0 mL 5%NaNO_(2),2.0 mL 10%Al(NO_(3))_(3),8.0 mL 8%NaOH;多糖最佳提取工艺:料液比1∶30(g/mL),20 min,回流提取2次;测定多糖的最佳显色条件:热水浴20 min,冷水浴15 min;以最佳提取工艺提取发酵48 h样品,测定样品总黄酮含量为2.4%,多糖含量为5.75%;方法学考察结果显示,新建总黄酮和多糖含量测定方法稳定,可行;样品的总黄酮和多糖含量在发酵48 h均达到峰值。结论:研究建立老范志万应神曲总黄酮和多糖含量测定方法;发酵过程中总黄酮和多糖的变化情况从侧面印证了老范志万应神曲传统炮制工艺的可行性和合理性。
文摘Heart failure （HF） is a major public health problem worldwide entailing high morbidity and mortality as well as high costs. This chronic syndrome associates with a low functional status and quality of life. Most patients with HF are elderly, constituting up to 80% of patients suffering from this disease with both incidence and prevalence of the condition increasing with age. This is due to the progressive aging of the population as well as improved and better survival after cardiac insults, such as myocardial infarction,
文摘The density of asphalt was measured with the pycnometer and densitometer. Creep properties of the asphalt were investigated with the bending beam rheometer at temperatures ranging from 0 ~C to -36 ~C. The asphalt density data used to correlate with the creep properties were calculated from the regression equation of density and temperature. The asphalt sample used to determine the creep property was aged by the standard RTFOT test and the PAV test. The test results showed that the asphalt density had a linear relationship with temperature changes. The logarithm of the creep stiffness and the slope of the logarithm of the stiffness at 60 seconds all demonstrated a linear relationship with the density, and the regression coefficient of these data was around 0.99. The creep stiffness and the slope of the creep stiffness can be calculated from the asphalt density at the same temperature.
