[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemu...[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemum rnorifolium petal using Trizol reagent, and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning. [Result] The sequencing result showed that the cloned cDNA sequence was 911 bp. It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family, and then named as CmXTH （gene accession number HM752243）. In addition, The BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs. Among of these, CmXTH shared closer genetic relationship with Gerbera jamesonii, Solanum lycopersicum, whereas had relatively distant relationship with Populus euparatica, Fragaria ananassa, Actinidia deliciosa, etc. [Conclusion] The cloned fragment was certainly the cDNA of XTH gene, which was associated with the petal growth and senescence in Chrysanthemum morifolium.展开更多
Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti...Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti-senescence activity, the expressions of p53, p21, p16 and Rb at the mRNA and protein levels were determined. Results showed that the expression of p53 was down-regulated significantly in a dose dependent manner after treatment with echinacoside. Further experiments suggested that the down-regulation of p53 may be correlated with the upregulation of SIRT1. In addition, echinacoside may exhibit considerable higher affinity towards SIRT1 than resveratrol, according to our molecular docking simulation. In conclusion, we expect that echinacoside might be a promising candidate for regulating cell senescence.展开更多
基金Supported by the Applied Basic Research Program of Suzhou City(SYN201205)the University Innovation Research and Training Program(101028537)~~
文摘[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemum rnorifolium petal using Trizol reagent, and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning. [Result] The sequencing result showed that the cloned cDNA sequence was 911 bp. It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family, and then named as CmXTH （gene accession number HM752243）. In addition, The BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs. Among of these, CmXTH shared closer genetic relationship with Gerbera jamesonii, Solanum lycopersicum, whereas had relatively distant relationship with Populus euparatica, Fragaria ananassa, Actinidia deliciosa, etc. [Conclusion] The cloned fragment was certainly the cDNA of XTH gene, which was associated with the petal growth and senescence in Chrysanthemum morifolium.
基金National Basic Research Program(973 Project) of China(Grant No.2007CB507406)the National Natural Science Foundation of China(Grant No.30572341)the Tsinghua-Yue-Yuen Medical Sciences Fund(THYY20070008)
文摘Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti-senescence activity, the expressions of p53, p21, p16 and Rb at the mRNA and protein levels were determined. Results showed that the expression of p53 was down-regulated significantly in a dose dependent manner after treatment with echinacoside. Further experiments suggested that the down-regulation of p53 may be correlated with the upregulation of SIRT1. In addition, echinacoside may exhibit considerable higher affinity towards SIRT1 than resveratrol, according to our molecular docking simulation. In conclusion, we expect that echinacoside might be a promising candidate for regulating cell senescence.
