Chlorfluazuron is an insect growth regulator for controlling the major insect pests in crops. This paper describes the effects of sublethal doses (LD10:1.00 ng/larva; LD30:3.75 ng/larva) of chlorfluazuron on ovari...Chlorfluazuron is an insect growth regulator for controlling the major insect pests in crops. This paper describes the effects of sublethal doses (LD10:1.00 ng/larva; LD30:3.75 ng/larva) of chlorfluazuron on ovarioles during ovarian development and oogenesis of common cutworm, Spodoptera litura (F.). It is a significant pest of cotton, tobacco and miscellaneous vegetables. The LDlo or LD3o was applied topically to the newly-ecdysed fifth-instars of S. litura under laboratory conditions. In controls, on 2nd day of adult emergence, the cell density in germarium was 1636 ± 9.17 nuclei/mm^2. It increased until 3rd day, i.e., 1829 ± 8.87 nuclei/mm^2 and decreased thereafter. In the LDloor LD-3o-treated insects, the patterns of the cell density was the same as in controls but values were significantly (P 〈 0.05) decreased in the LD10- and more in the LDa0-treated females compared with controls. In control, ovaries were normal type and had mature ova with an occasional one or two being absorbed. In LD10-treated females, ovaries had spaces in ovarioles and absorption of ova. In LD30-treated females, besides the spacing and absorption, sometimes only immature (germarium) ova were found.展开更多
The present study investigated the effects and underlying mechanism of ethylacetate fraction of Ribes fasciculatum (ERF) on the lifespan and stress tolerance using a Caenorhabditis elegans model. The longevity activ...The present study investigated the effects and underlying mechanism of ethylacetate fraction of Ribes fasciculatum (ERF) on the lifespan and stress tolerance using a Caenorhabditis elegans model. The longevity activity of ERF was determined by lifespan assay under normal culture condition. The survival rate of nematodes under various stress conditions was assessed to validate the effects of ERF on the stress tolerance. To determine the antioxidant potential of ERF, the superoxide dismutase (SOD) activities and intracellular reactive oxygen species (ROS) levels were investigated. The ERF-mediated change in SOD-3 expression was examined using GFP-expressing transgenic strain. The effects of ERF on the aging-related factors were investigated by reproduction assay and pharyngeal pumping assay. The intestinal lipofuscin levels of aged nematodes were also measured. The mechanistic studies were performed using selected mutant strains. Our results indicated that ERF showed potent lifespan extension effects on the wild-type nematode under both normal and various stress conditions. The ERF treatment also enhanced the activity and expression of superoxide dismutase (SOD) and attenuated the intracellular ROS levels. Moreover, ERF-fed nematodes showed decreased lipofuscin accumulation, indicating ERF might affect age-associated changes in C. elegans. The results of mechanistic studies indicated that there was no significant lifespan extension in ERF-treated daf-2, age-l, sir-2.1, and daf-16 null mutants, suggesting that they were involved in ERF-mediated lifespan regulation. In conclusion, R. fasciculatum confers increased longevity and stress resistance in C. elegans via SIR-2.1-mediated DAF-16 activation, dependent on the insulin/1GF signaling pathway.展开更多
Dietary restriction(DR) is one of the most robust environmental manipulations that not only extend life span but also delay the onset of age-related diseases in almost every species examined. Caenorhabditis elegans pl...Dietary restriction(DR) is one of the most robust environmental manipulations that not only extend life span but also delay the onset of age-related diseases in almost every species examined. Caenorhabditis elegans plays an important role in aging studies due to its simple life cycle, easy genetic manipulations and highly conserved genome. Recent studies have demonstrated that the beneficial effects of DR are mediated by the highly conserved transcription factors and signaling pathways in C. elegans. Here we review recent progress in the methodology and molecular mechanisms of DR using C. elegans as a model, as well as prospects for future research.展开更多
β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less of...β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less often.In the present study,we investigated the interaction and toxicity of Aβ1-42 and copper in the Aβ1-42 transgenic Caenorhabditis elegans worm model CL2006.Our data show that the paralysis behavior of CL2006 worms significantly deteriorated after exposure to 10-3 mol L-1 copper ions.However,the paralysis behavior was dramatically attenuated with exposure to 10-4 mol L-1 copper ions.The exogenous copper treatment also partially changed the homeostatic balance of zinc,manganese,and iron.Our data suggest that the accumulation of reactive oxygen species (ROS) was responsible for the paralysis induced by Aβ and copper in CL2006.The ROS generation induced by Aβ and copper appear to be through sod-1,prdx-2,skn-1,hsp-60 and hsp-16.2 genes.展开更多
文摘Chlorfluazuron is an insect growth regulator for controlling the major insect pests in crops. This paper describes the effects of sublethal doses (LD10:1.00 ng/larva; LD30:3.75 ng/larva) of chlorfluazuron on ovarioles during ovarian development and oogenesis of common cutworm, Spodoptera litura (F.). It is a significant pest of cotton, tobacco and miscellaneous vegetables. The LDlo or LD3o was applied topically to the newly-ecdysed fifth-instars of S. litura under laboratory conditions. In controls, on 2nd day of adult emergence, the cell density in germarium was 1636 ± 9.17 nuclei/mm^2. It increased until 3rd day, i.e., 1829 ± 8.87 nuclei/mm^2 and decreased thereafter. In the LDloor LD-3o-treated insects, the patterns of the cell density was the same as in controls but values were significantly (P 〈 0.05) decreased in the LD10- and more in the LDa0-treated females compared with controls. In control, ovaries were normal type and had mature ova with an occasional one or two being absorbed. In LD10-treated females, ovaries had spaces in ovarioles and absorption of ova. In LD30-treated females, besides the spacing and absorption, sometimes only immature (germarium) ova were found.
基金supported by a grant from Woosuk University(2013)
文摘The present study investigated the effects and underlying mechanism of ethylacetate fraction of Ribes fasciculatum (ERF) on the lifespan and stress tolerance using a Caenorhabditis elegans model. The longevity activity of ERF was determined by lifespan assay under normal culture condition. The survival rate of nematodes under various stress conditions was assessed to validate the effects of ERF on the stress tolerance. To determine the antioxidant potential of ERF, the superoxide dismutase (SOD) activities and intracellular reactive oxygen species (ROS) levels were investigated. The ERF-mediated change in SOD-3 expression was examined using GFP-expressing transgenic strain. The effects of ERF on the aging-related factors were investigated by reproduction assay and pharyngeal pumping assay. The intestinal lipofuscin levels of aged nematodes were also measured. The mechanistic studies were performed using selected mutant strains. Our results indicated that ERF showed potent lifespan extension effects on the wild-type nematode under both normal and various stress conditions. The ERF treatment also enhanced the activity and expression of superoxide dismutase (SOD) and attenuated the intracellular ROS levels. Moreover, ERF-fed nematodes showed decreased lipofuscin accumulation, indicating ERF might affect age-associated changes in C. elegans. The results of mechanistic studies indicated that there was no significant lifespan extension in ERF-treated daf-2, age-l, sir-2.1, and daf-16 null mutants, suggesting that they were involved in ERF-mediated lifespan regulation. In conclusion, R. fasciculatum confers increased longevity and stress resistance in C. elegans via SIR-2.1-mediated DAF-16 activation, dependent on the insulin/1GF signaling pathway.
基金supported by grants from the National Natural Science Foundation of China(31471379)Natural Science Foundation for Universities in Jiangsu Province,China(BK2014021506)to Chen Di
文摘Dietary restriction(DR) is one of the most robust environmental manipulations that not only extend life span but also delay the onset of age-related diseases in almost every species examined. Caenorhabditis elegans plays an important role in aging studies due to its simple life cycle, easy genetic manipulations and highly conserved genome. Recent studies have demonstrated that the beneficial effects of DR are mediated by the highly conserved transcription factors and signaling pathways in C. elegans. Here we review recent progress in the methodology and molecular mechanisms of DR using C. elegans as a model, as well as prospects for future research.
基金supported by the National Natural Science Foundation of China (Grant No. 30870578)the National Basic Research Program of China (Grant No. 2006CB500700)funded by the US National Institutes of Health for providing nematode strains used in this work
文摘β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less often.In the present study,we investigated the interaction and toxicity of Aβ1-42 and copper in the Aβ1-42 transgenic Caenorhabditis elegans worm model CL2006.Our data show that the paralysis behavior of CL2006 worms significantly deteriorated after exposure to 10-3 mol L-1 copper ions.However,the paralysis behavior was dramatically attenuated with exposure to 10-4 mol L-1 copper ions.The exogenous copper treatment also partially changed the homeostatic balance of zinc,manganese,and iron.Our data suggest that the accumulation of reactive oxygen species (ROS) was responsible for the paralysis induced by Aβ and copper in CL2006.The ROS generation induced by Aβ and copper appear to be through sod-1,prdx-2,skn-1,hsp-60 and hsp-16.2 genes.