The aim of this study is to investigate epigenetic mechanism of ABCG2 induced drug-resistance. It is not only expatiate for drug-resistance regulation mechanism in all-round, but also to provide scientific experimenta...The aim of this study is to investigate epigenetic mechanism of ABCG2 induced drug-resistance. It is not only expatiate for drug-resistance regulation mechanism in all-round, but also to provide scientific experimental basis for selecting target to reverse its drug-resistance. Apply methylation-specific PCR （MSP） to have tested methylation of ABCG2 promoter region -359 to -353 specific positions in breast cancer tissues and paired adjacent tissue of 22 cases and test their methylation positions with MSP products for sequencing; and adopt fluorescent quantitation RT-PCR to test expression level DNMT1, DNMT3A, DNMT3B and ABCG2; to make analysis on relationship between them with statistical spearman correlation. Specific positions of ABCG2 gene promoter region of 18 cases among the 22 cases with breast cancer （18/22, 82%） existed high methylation （P〈0.05）, MSP products sequencing proved methylation of the specific position, and mRNA expression level was relative higher in remarkable positive correlation （P〈0.05） ABCG2, DNMT1, DNMT3A, DNMT3B mRNA expression levels in breast cancer tissues were obviously higher than adjacent tissues （P〈0.01）, and DNMT3B expression level was obviously higher than DNMT1 and DNMT3A （P〈0.01） in negative correlation with ABCG2 gene expression （P=0.001）. -359 to -353 positions of promoter regions of ABCG2gene existed high methylation capable to push expression of this gene in beast cancer tissue. DNMT3B is involved in expression regulation in ABCG2 gene, and provides new scientific basis for drug-resistance target as reverse ABCG2 induction展开更多
Axial compression tests were conducted on AZ31 B magnesium and A6063 aluminum thin-walled square tubes with varied lengths and induced features at different compression rates. In compression, the magnesium tubes exhib...Axial compression tests were conducted on AZ31 B magnesium and A6063 aluminum thin-walled square tubes with varied lengths and induced features at different compression rates. In compression, the magnesium tubes exhibited a "local buckling and fracture" mode, with three fracture patterns, i.e."horizontal","double-oblique", and "spiral" fractures. In general, the magnesium tube showed an inferior crashworthiness to the aluminum square tube. In addition, the effects of L/W ratio, strain rate and induced features on the crashworthiness of thin-walled square tubes were investigated. With an increase in the L/W ratio(L and W represent the tube length and width, respectively) from 1 to 4, the maximal force and global specific energy absorption decreased in a power-law trend for the magnesium tubes,while they remained approximately constant for the aluminum tubes. Furthermore, as the compression rate increased from 5×10-5 to 10 m/s, the primary crashworthiness parameters of the magnesium tubes increased in an approximately exponential manner,while for the aluminum tubes,they changed slightly. Finally,the involved induced features were proven to be not an effective method to improve the specific energy absorption of magnesium tubes, thus, more trigger types,locations,and sizes will be evaluated in future to improve the energy-absorption ability.展开更多
This study aimed to induce callus from three sunflower （Helianthus annulus L.） hybrids, namely Anna, Alhaja and Kuds, and to evaluate their callus for salt stress tolerance. Cotyledons and hypocotyl were taken from ...This study aimed to induce callus from three sunflower （Helianthus annulus L.） hybrids, namely Anna, Alhaja and Kuds, and to evaluate their callus for salt stress tolerance. Cotyledons and hypocotyl were taken from seedling of these hybrids and cultured on MS media contained 2,4-D （0,0, 0.5, 1.0, 1.5 and 2.0 mg/L） and kinetin （0.0, 0.5, and 1.0 mg/L）. The cultures incubated at 25 ＋ I^C under light condition （1,000 Lux） for 16 h/day. After 6 weeks observations were taken on the response of cotyledons and hypocotyl to callus induction. The induced callus were cultured on the same MS media that contained appropriate concentrations of 2,4-D and kinetin for callus induction as well as contained various concentration of sodium chloride NaCI （0.0, 0.05, 0.1, 0.15 and 0.2%）. After six weeks callus fresh and dry weights, proline and total carbohydrates concentrations were measured. The results showed significant differences among the hybrids, explants, 2,4-D and kinetin concentration and significant interaction between them in their percentage response for callus induction. The results also revealed that fresh and dry weights were significantly reduced with increased NaCI concentration in the medium, hybrids showed significant differences in their response to salt stress. Proline and total carbohydrate concentration increased in callus as NaCI increased in the media. Significant interaction was showed between hybrids and NaCI concentration in these parameters.展开更多
基金Supported by the National Natural Science Foundation of China (30500599 and 30571592)the Natural Science Foundation of Guangdong (9151503102000019)the Medical Scientific Research Foundation of Guangdong (A2009606)
文摘The aim of this study is to investigate epigenetic mechanism of ABCG2 induced drug-resistance. It is not only expatiate for drug-resistance regulation mechanism in all-round, but also to provide scientific experimental basis for selecting target to reverse its drug-resistance. Apply methylation-specific PCR （MSP） to have tested methylation of ABCG2 promoter region -359 to -353 specific positions in breast cancer tissues and paired adjacent tissue of 22 cases and test their methylation positions with MSP products for sequencing; and adopt fluorescent quantitation RT-PCR to test expression level DNMT1, DNMT3A, DNMT3B and ABCG2; to make analysis on relationship between them with statistical spearman correlation. Specific positions of ABCG2 gene promoter region of 18 cases among the 22 cases with breast cancer （18/22, 82%） existed high methylation （P〈0.05）, MSP products sequencing proved methylation of the specific position, and mRNA expression level was relative higher in remarkable positive correlation （P〈0.05） ABCG2, DNMT1, DNMT3A, DNMT3B mRNA expression levels in breast cancer tissues were obviously higher than adjacent tissues （P〈0.01）, and DNMT3B expression level was obviously higher than DNMT1 and DNMT3A （P〈0.01） in negative correlation with ABCG2 gene expression （P=0.001）. -359 to -353 positions of promoter regions of ABCG2gene existed high methylation capable to push expression of this gene in beast cancer tissue. DNMT3B is involved in expression regulation in ABCG2 gene, and provides new scientific basis for drug-resistance target as reverse ABCG2 induction
基金Project(2017JBM041)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(51505024)supported by the National Natural Science Foundation of ChinaProject supported by the Foundation of Zhejiang Key Laboratory of Automobile Safety Technology of China
文摘Axial compression tests were conducted on AZ31 B magnesium and A6063 aluminum thin-walled square tubes with varied lengths and induced features at different compression rates. In compression, the magnesium tubes exhibited a "local buckling and fracture" mode, with three fracture patterns, i.e."horizontal","double-oblique", and "spiral" fractures. In general, the magnesium tube showed an inferior crashworthiness to the aluminum square tube. In addition, the effects of L/W ratio, strain rate and induced features on the crashworthiness of thin-walled square tubes were investigated. With an increase in the L/W ratio(L and W represent the tube length and width, respectively) from 1 to 4, the maximal force and global specific energy absorption decreased in a power-law trend for the magnesium tubes,while they remained approximately constant for the aluminum tubes. Furthermore, as the compression rate increased from 5×10-5 to 10 m/s, the primary crashworthiness parameters of the magnesium tubes increased in an approximately exponential manner,while for the aluminum tubes,they changed slightly. Finally,the involved induced features were proven to be not an effective method to improve the specific energy absorption of magnesium tubes, thus, more trigger types,locations,and sizes will be evaluated in future to improve the energy-absorption ability.
文摘This study aimed to induce callus from three sunflower （Helianthus annulus L.） hybrids, namely Anna, Alhaja and Kuds, and to evaluate their callus for salt stress tolerance. Cotyledons and hypocotyl were taken from seedling of these hybrids and cultured on MS media contained 2,4-D （0,0, 0.5, 1.0, 1.5 and 2.0 mg/L） and kinetin （0.0, 0.5, and 1.0 mg/L）. The cultures incubated at 25 ＋ I^C under light condition （1,000 Lux） for 16 h/day. After 6 weeks observations were taken on the response of cotyledons and hypocotyl to callus induction. The induced callus were cultured on the same MS media that contained appropriate concentrations of 2,4-D and kinetin for callus induction as well as contained various concentration of sodium chloride NaCI （0.0, 0.05, 0.1, 0.15 and 0.2%）. After six weeks callus fresh and dry weights, proline and total carbohydrates concentrations were measured. The results showed significant differences among the hybrids, explants, 2,4-D and kinetin concentration and significant interaction between them in their percentage response for callus induction. The results also revealed that fresh and dry weights were significantly reduced with increased NaCI concentration in the medium, hybrids showed significant differences in their response to salt stress. Proline and total carbohydrate concentration increased in callus as NaCI increased in the media. Significant interaction was showed between hybrids and NaCI concentration in these parameters.
