A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperat...A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperatures ranging from 30℃ to 50℃ with the highest activity at 45℃ and pH7.2. Sodium chloride increased its activity markedly, and KC1 increased its activity slightly. The divalent and trivalent metal ions including Cu^2＋, Ni^2＋, Zn^2＋, Mn^2＋, Al^3＋ and Fe^3＋ significantly inhibited its activity, while Mg^2＋ did not. CgkP remained 70% of original activity after being incubated at 40℃ for 48h, and remained 80% of the activity after being incubated at 45℃ for 1 h. It exhibited endo-κ-carrageenase activity, mainly depolymerizing the κ-carrageenan into disaccharide and tetrasaccharide. CgkP was more thermostable than most of previously reported κ-carrageenases with a potential of being used in industry.展开更多
There is low formation pressure coefficient and high formation temperature in ludong-wucaiwan area. Gas cut and gas channeling happen seriously during oil and gas well cementing. The existing anti-migration additive h...There is low formation pressure coefficient and high formation temperature in ludong-wucaiwan area. Gas cut and gas channeling happen seriously during oil and gas well cementing. The existing anti-migration additive has only effects on single trait, so it is difficult to meet cementing requirement. According to this situation we could use latex slurry to anti-gas channeling. We have synthesised a set of anti-gas channeling lightweight temperature-resistant latex slurry and formed a new channeling preventing latex slurry through plenty of previous laboratory experiments. Finally the performance of latex slurry on temperature-resistant, anti-gas channeling and the anti-gas channeling of cement paste are studied. The experimental results show that this latex system has strong temperature-resistant and anti-gas channeling, which completely meet the requirement of cementing in this area.展开更多
基金supported by National Science Foundation of China (31000361 and 31070712)Program for Changjiang Scholars and Innovative Research Team in University (IRT0944)+1 种基金Special Fund for Marine Scientific Research in the Public Interest (201005024)the Fundamental Research Funds for the Central Universities(201013008)
文摘A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperatures ranging from 30℃ to 50℃ with the highest activity at 45℃ and pH7.2. Sodium chloride increased its activity markedly, and KC1 increased its activity slightly. The divalent and trivalent metal ions including Cu^2＋, Ni^2＋, Zn^2＋, Mn^2＋, Al^3＋ and Fe^3＋ significantly inhibited its activity, while Mg^2＋ did not. CgkP remained 70% of original activity after being incubated at 40℃ for 48h, and remained 80% of the activity after being incubated at 45℃ for 1 h. It exhibited endo-κ-carrageenase activity, mainly depolymerizing the κ-carrageenan into disaccharide and tetrasaccharide. CgkP was more thermostable than most of previously reported κ-carrageenases with a potential of being used in industry.
文摘There is low formation pressure coefficient and high formation temperature in ludong-wucaiwan area. Gas cut and gas channeling happen seriously during oil and gas well cementing. The existing anti-migration additive has only effects on single trait, so it is difficult to meet cementing requirement. According to this situation we could use latex slurry to anti-gas channeling. We have synthesised a set of anti-gas channeling lightweight temperature-resistant latex slurry and formed a new channeling preventing latex slurry through plenty of previous laboratory experiments. Finally the performance of latex slurry on temperature-resistant, anti-gas channeling and the anti-gas channeling of cement paste are studied. The experimental results show that this latex system has strong temperature-resistant and anti-gas channeling, which completely meet the requirement of cementing in this area.
