Isolation, identification and characterization of cadmium-resistant Pseudomonas aeruginosa strain E_1

Strain E1 with resistance to 18 mmol/L cadmium （Cd）, isolated from Cd-contaminated soil was identified by morphological observation, biochemical and physiological characterization and 16S rDNA sequence analysis. The resistance to heavy metals Cd, Cu, Co, Mn, Pb, Zn and 12 antibiotics was examined. The ability of removing Cd from solution was studied. The characterizations show that strain El is affiliated to Pseudomonas aeruginosa （P aeruginosa）. Strain E1 has high resistance to heavy metals and the order is found to be Cd〉Mn〉Zn〉Cu〉Pb〉Co in solid media. Strain E1 also exhibits the resistance to 12 antibiotics. Both living and non-living cells of strain E1 can remove Cd from solution, and living cell has better biosorption than non-living cell.

Sensitivity Study on Durability Variables of Marine Concrete Structures

In order to study the influence of parameters on durability of marine concrete structures, the parameter＇s sensitivity analysis was studied in this paper. With the Fick＇s 2nd law of diffusion and the deterministic sensitivity analysis method （DSA）, the sensitivity factors of apparent surface chloride content, apparent chloride diffusion coefficient and its time dependent attenuation factor were analyzed. The results of the analysis show that the impact of design variables on concrete durability was different. The values of sensitivity factor of chloride diffusion coefficient and its time dependent attenuation factor were higher than others. Relative less error in chloride diffusion coefficient and its time dependent attenuation coefficient induces a bigger error in concrete durability design and life prediction. According to probability sensitivity analysis （PSA）, the influence of mean value and variance of concrete durability design variables on the durability failure probability was studied. The results of the study provide quantitative measures of the importance of concrete durability design and life prediction variables. It was concluded that the chloride diffusion coefficient and its time dependent attenuation factor have more influence on the reliability of marine concrete structural durability. In durability design and life prediction of marine concrete structures, it was very important to reduce the measure and statistic error of durability design variables.

抗分散透水性混凝土性能研究

对透水混凝土的水泥浆进行改性,得出耐离析、高流动性能的透水混凝土。通过室内研究系统研究了灰骨比、水灰比、骨料级配对透水混凝土强度、密度、孔隙率、渗透性的影响规律。试验结果表明:抗分散剂可有效解决水泥浆下沉及离析的问题;抗分散透水混凝土的灰骨比对混凝土的性能有显著影响,水灰比的影响较小;集料级配对混凝土的强度及孔隙率具有一定的影响。基于上述试验,得出适用于现场施工的透水混凝土配合比,通过现场试验验证了振动沉管法施工透水性混凝土桩的可行性,并验证了透水性混凝土桩具有较高承载力。