With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problem...With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problems to use phage controlling disease.Phage is a kind of prokaryotic virus,widely exists in the nature and includes bacteriophage,cyanophage and actinophage.Due to its potential of replacing antibiotics to treat disease,phage receives more and more attention.In this paper,based on development status of phage research at home and abroad,discovery process,naming method and classification basis of phage are introduced comprehensively,and advantages and limitations of phage applying in prevention and control of bacterial diseases are analyzed.We introduce application status of phage in human medicine,prevention and control of diseases for terrestrial animals and aquaculture,and the effects of phage in sewage treatment,prevention and control of microbial contamination of food and detection technology,and point out the shortages of phage in the above application.Meanwhile,we also discuss application prospects of phage in disease prevention and control,environmental protection and food safety.展开更多
The emergence of antibiotic resistance in bacteria is a major public-health issue.Synthesis of efficient antibiotic-free material is very important for fighting bacterial infection-related diseases.Herein,red-carbon d...The emergence of antibiotic resistance in bacteria is a major public-health issue.Synthesis of efficient antibiotic-free material is very important for fighting bacterial infection-related diseases.Herein,red-carbon dots(R-CDs)with a broad range of spectral absorption(350–700 nm)from organic bactericides or intermediates were synthesized through a solvothermal route.The prepared R-CDs not only had intrinsic antibacterial activities,but also could kill multidrug-resistant bacteria(multidrug-resistant Acinetobacter baumannii(MRAB)and multidrug-resistant Staphylococcus aureus(MRSA))effectively by generating reactive oxygen species.Furthermore,R-CDs could eliminate and inhibit the formation of MRAB biofilms,while conferring few side effects on normal cells.A unique property of R-CDs was demonstrated upon in vivo treatment of antibiotic-sensitive MRABinduced infected wounds.These data suggested that this novel R-CDs-based strategy might enable the design of nextgeneration agents to fight drug-resistant bacteria.展开更多
Bacterial infections are grave threats to human health,particularly those caused by the most common Grampositive bacteria.The massive administration of broad-spectrum antibiotics to treat various bacterial infections ...Bacterial infections are grave threats to human health,particularly those caused by the most common Grampositive bacteria.The massive administration of broad-spectrum antibiotics to treat various bacterial infections has led to the evolution and spread of drug resistance.As a universal antimicrobial technique unapt to induce drug resistance,photothermal therapy(PTT)is attracting extensive attention in recent years.However,its unspecific killing capability and side effects towards adjacent mammalian cells severely impede the practical applications.Herein,we proposed a metabolic engineering strategy to selectively inactivate Gram-positive bacteria by PTT.A bioorthogonal photothermal agent was prepared by the conjugation of IR-780 iodide and dibenzocyclooctyne(IR780-DBCO).Upon pre-metabolizing with 3-azido-D-alanine,Gram-positive bacteria rather than Gramnegative ones,such as Staphylococcus aureus and vancomycinresistant Enterococcus faecalis(VRE),could be specifically tied up by the explosive IR780-DBCO via copper-free click chemistry.Thereafter,they spontaneously detonated under 15 min near-infrared light irradiation and inactivated nearly 100% Gram-positive bacteria in vitro.Moreover,superbug VRE-induced infection was significantly inhibited by this approach in a mouse skin wound model.This metabolic labelling-based photothermal ablation strategy specific to Gram-positive microbes would stimulate the development of precise antibacterial candidates for preclinical applications.展开更多
基金Supported by Special Fund for Scientific Research in Marine Public Welfare Industry(201405003)
文摘With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problems to use phage controlling disease.Phage is a kind of prokaryotic virus,widely exists in the nature and includes bacteriophage,cyanophage and actinophage.Due to its potential of replacing antibiotics to treat disease,phage receives more and more attention.In this paper,based on development status of phage research at home and abroad,discovery process,naming method and classification basis of phage are introduced comprehensively,and advantages and limitations of phage applying in prevention and control of bacterial diseases are analyzed.We introduce application status of phage in human medicine,prevention and control of diseases for terrestrial animals and aquaculture,and the effects of phage in sewage treatment,prevention and control of microbial contamination of food and detection technology,and point out the shortages of phage in the above application.Meanwhile,we also discuss application prospects of phage in disease prevention and control,environmental protection and food safety.
基金supported by the National Natural Science Foundation of China(NSFC,21925802,21878039,21808028,22022803 and 22078046)the NSFC-Liaoning United Fund(U1908202)the National Key Research and Development Plan(2018AAA0100301)。
文摘The emergence of antibiotic resistance in bacteria is a major public-health issue.Synthesis of efficient antibiotic-free material is very important for fighting bacterial infection-related diseases.Herein,red-carbon dots(R-CDs)with a broad range of spectral absorption(350–700 nm)from organic bactericides or intermediates were synthesized through a solvothermal route.The prepared R-CDs not only had intrinsic antibacterial activities,but also could kill multidrug-resistant bacteria(multidrug-resistant Acinetobacter baumannii(MRAB)and multidrug-resistant Staphylococcus aureus(MRSA))effectively by generating reactive oxygen species.Furthermore,R-CDs could eliminate and inhibit the formation of MRAB biofilms,while conferring few side effects on normal cells.A unique property of R-CDs was demonstrated upon in vivo treatment of antibiotic-sensitive MRABinduced infected wounds.These data suggested that this novel R-CDs-based strategy might enable the design of nextgeneration agents to fight drug-resistant bacteria.
基金supported by the National Natural Science Foundation of China(52003222 and 21875189)Ningbo Natural Science Foundation(202003N4064)+2 种基金the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0752)the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-013)the Fundamental Research Funds for the Central Universities.
文摘Bacterial infections are grave threats to human health,particularly those caused by the most common Grampositive bacteria.The massive administration of broad-spectrum antibiotics to treat various bacterial infections has led to the evolution and spread of drug resistance.As a universal antimicrobial technique unapt to induce drug resistance,photothermal therapy(PTT)is attracting extensive attention in recent years.However,its unspecific killing capability and side effects towards adjacent mammalian cells severely impede the practical applications.Herein,we proposed a metabolic engineering strategy to selectively inactivate Gram-positive bacteria by PTT.A bioorthogonal photothermal agent was prepared by the conjugation of IR-780 iodide and dibenzocyclooctyne(IR780-DBCO).Upon pre-metabolizing with 3-azido-D-alanine,Gram-positive bacteria rather than Gramnegative ones,such as Staphylococcus aureus and vancomycinresistant Enterococcus faecalis(VRE),could be specifically tied up by the explosive IR780-DBCO via copper-free click chemistry.Thereafter,they spontaneously detonated under 15 min near-infrared light irradiation and inactivated nearly 100% Gram-positive bacteria in vitro.Moreover,superbug VRE-induced infection was significantly inhibited by this approach in a mouse skin wound model.This metabolic labelling-based photothermal ablation strategy specific to Gram-positive microbes would stimulate the development of precise antibacterial candidates for preclinical applications.
