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药物联用作用方法评价
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作者 侯小莉 柴程芝 +1 位作者 段英丹 余伯阳 《临床心身疾病杂志》 CAS 2016年第S2期114-115,共2页
药物联合使用能达到增效、减毒和减少耐药性的效果,临床上在治疗复杂疾病时应用已非常普遍。本文对药物联用相互作用的评价方法及应用进行综述,通过介绍不同方法的优缺点,以期为药物联用的作用评价研究提供参考。
关键词 药物联用 评价方法 联用指数
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TPL与5-FU联合作用对人结肠癌HT-29细胞增殖与凋亡的影响 被引量:2
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作者 汤小燕 朱尤庆 +1 位作者 林小玲 刘诗权 《武汉大学学报(医学版)》 CAS 2007年第4期441-445,共5页
目的:研究雷公藤内酯醇(TPL)与5-氟尿嘧啶(5-FU)联合应用对人结肠癌HT-29细胞增殖、凋亡的影响。方法:常规培养HT-29细胞,MTT比色法检测不同浓度TPL与5-FU单独或联合应用对HT-29细胞的增殖抑制率,透射电镜在亚细胞结构形态上证实凋亡细... 目的:研究雷公藤内酯醇(TPL)与5-氟尿嘧啶(5-FU)联合应用对人结肠癌HT-29细胞增殖、凋亡的影响。方法:常规培养HT-29细胞,MTT比色法检测不同浓度TPL与5-FU单独或联合应用对HT-29细胞的增殖抑制率,透射电镜在亚细胞结构形态上证实凋亡细胞,流式细胞学定量分析凋亡细胞比例,免疫细胞化学分析突变型P53、bcl-2蛋白表达的变化。结果:①TPL与5-FU单独应用均能抑制HT-29细胞的增殖,且在一定范围内存在浓度依赖性。两药联合作用最高增殖抑制率最高达到(94.92±2.76)%,48 h凋亡率达(41.71±1.38)%。②TPL与5-FU在低剂量联合应用时对HT-29细胞增殖抑制作用有协同效应(CI<1),其机制可能为促进细胞凋亡。③与对照组比较,TPL与5-FU单独应用及联合应用后HT-29细胞突变型P53蛋白表达率均有显著降低,而bcl-2蛋白表达没有明显变化。结论:TPL与5-FU在小剂量联合应用时为协同效应,可能通过下调突变型P53蛋白诱导凋亡从而抑制细胞生长。本结果对于结肠癌的治疗具有一定的临床意义。 展开更多
关键词 雷公藤内酯醇 5-氟尿嘧啶 协同效应 凋亡 联用指数
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Identification of Volatile Compounds in Codfish(Gadus) by a Combination of Two Extraction Methods Coupled with GC-MS Analysis 被引量:5
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作者 CHANG Yufei HOU Hu LI Bafang 《Journal of Ocean University of China》 SCIE CAS 2016年第3期509-514,共6页
Codfish is a kind of abyssal fish species with a great value in food industry. However, the flavor of codfish, especially the unpleasant odor, has caused serious problems in its processing. To accurately identify the ... Codfish is a kind of abyssal fish species with a great value in food industry. However, the flavor of codfish, especially the unpleasant odor, has caused serious problems in its processing. To accurately identify the volatile compounds in codfish, a combination of solid phase micro-extraction(SPME) method and simultaneous distillation extraction(SDE) method was used to extract the volatiles. Gas chromatography-mass spectrometry(GC-MS) along with Kovats indices(KI) and authentic standard compounds were used to identify the volatiles. The results showed that a total of 86 volatile compounds were identified in codfish, of them 24 were extracted by SDE, 69 compounds by SPME, and 10 compounds by both SDE and SPME. Seventy volatile compounds were found to have specific odors, of them 7 typical compounds contributed significantly to the flavor of codfish. Alcohols(i.e.,(E)-2-penten-1-ol and 2-octanol), esters(i.e., ethyl butyrate and methyl geranate), aldehydes(i.e., 2-dodecenal and pentadecanal) contributed the most to fresh flavor while nitrogen compounds, sulphur compounds, furans, as well as some ketones(i.e., 2-hydroxy-3-pentanone) brought unpleasant odor, such as fishy and earthy odor. It was indicated that the combination of multiple extraction methods and GC-MS analysis can enhance the accuracy of identification, and provide a reference for the further study on flavor of aquatic products. 展开更多
关键词 codfish volatile flavor compound SDE SPME GC-MS Kovats index
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The Study on the Economic Application of Financial Big Data
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作者 Xue Rui Wang Chengcheng Zhou Meihui 《Review of Global Academics》 2014年第4期310-311,共2页
Big Data has become the focus of the Internet industry. With the development of various industries becoming more sophisticated, long-term accumulation of operational data, customer data displays exponential growth tre... Big Data has become the focus of the Internet industry. With the development of various industries becoming more sophisticated, long-term accumulation of operational data, customer data displays exponential growth trend. The rapid development of financial big data both poses opportunities and challenges for financial industry and economic growth. This article explains the economic application of big data based on the perspective of economics. 展开更多
关键词 Big Data Economic Application Financial Industry
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