The effects of sodium hypochlorite concentration in the recovery of poly-β-hydroxybutyrate (PHB) are investigated. A simple reaction equation and a mathematical model describe the reaction kinetics. Results showed th...The effects of sodium hypochlorite concentration in the recovery of poly-β-hydroxybutyrate (PHB) are investigated. A simple reaction equation and a mathematical model describe the reaction kinetics. Results showed that the stoichiometric coefficient increases with increasing CAO/CBO, where CAO is the concentration of the active chlorine at t = 0 and CBO is the initial concentration of the non-PHB materials.展开更多
Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage A with S amber mutation (S-RRz) and poly(B-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coli cell, simultaneousl...Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage A with S amber mutation (S-RRz) and poly(B-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coli cell, simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored ph&CAB only. The simultaneous expression of vgb and S- RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB.展开更多
The basic objective of this research is to determine the effects of acetate, propionate and butyrate on the biological phosphate(bio p) release and poly β hydroxybutyrate(PHB) synthesis and...The basic objective of this research is to determine the effects of acetate, propionate and butyrate on the biological phosphate(bio p) release and poly β hydroxybutyrate(PHB) synthesis and to test the effects of different acetate concentrations on the bio p release as well as the effect of butyrate on the bio p release and PHB synthesis in the presence of nitrates. Oxidation reduction potential probes are used to monitor the relative anaerobic and anoxic states of the sewage. The author quantified PHB, PHV in activated sludge by gas liquid chromatography. It has been demonstrated through a series of batch experiments that phosphorus release and PHB synthesis both take place in anaerobic zone of the bio p removal process, and the key factors to the maximization of PHB synthesis are that simple carbon substrates should be added to the anaerobic zone and that the addition of electron acceptors should be avoided.展开更多
文摘The effects of sodium hypochlorite concentration in the recovery of poly-β-hydroxybutyrate (PHB) are investigated. A simple reaction equation and a mathematical model describe the reaction kinetics. Results showed that the stoichiometric coefficient increases with increasing CAO/CBO, where CAO is the concentration of the active chlorine at t = 0 and CBO is the initial concentration of the non-PHB materials.
基金Supported by the National Natural Science Foundation of China (No. 29834103, 29876021).
文摘Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage A with S amber mutation (S-RRz) and poly(B-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coli cell, simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored ph&CAB only. The simultaneous expression of vgb and S- RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB.
文摘The basic objective of this research is to determine the effects of acetate, propionate and butyrate on the biological phosphate(bio p) release and poly β hydroxybutyrate(PHB) synthesis and to test the effects of different acetate concentrations on the bio p release as well as the effect of butyrate on the bio p release and PHB synthesis in the presence of nitrates. Oxidation reduction potential probes are used to monitor the relative anaerobic and anoxic states of the sewage. The author quantified PHB, PHV in activated sludge by gas liquid chromatography. It has been demonstrated through a series of batch experiments that phosphorus release and PHB synthesis both take place in anaerobic zone of the bio p removal process, and the key factors to the maximization of PHB synthesis are that simple carbon substrates should be added to the anaerobic zone and that the addition of electron acceptors should be avoided.
