Through reducing incubation time,the low-level lactosed polyethyleneimine(L-PEI)was prepared by reductive amination of PEI with lactose.The content of galactose residue in the complex was determined with phenol-sulfur...Through reducing incubation time,the low-level lactosed polyethyleneimine(L-PEI)was prepared by reductive amination of PEI with lactose.The content of galactose residue in the complex was determined with phenol-sulfuric acid method and the lactose rate was calculated.The result shows that:a good linear relationship within galactose concentration range of 1.25～12.5μg/mL(r=0.996),RSD of reappearance was 2.16%,the average rate of recoveries was 92.5%(RSD=2.18%).With a 6.27% lactose rate,the developed L-PEI reached its expected level.展开更多
利用荧光技术创建一种新的方法评价经交联处理异体组织的交联程度。以新西兰兔胸主动脉作为研究对象,按与其作用的戊二醛(GA)浓度不同分为空白对照组--未与GA作用(A组),GA浓度0.625%组(B组),GA浓度1.25%组(C组),GA浓度2.5%组(D组),各组...利用荧光技术创建一种新的方法评价经交联处理异体组织的交联程度。以新西兰兔胸主动脉作为研究对象,按与其作用的戊二醛(GA)浓度不同分为空白对照组--未与GA作用(A组),GA浓度0.625%组(B组),GA浓度1.25%组(C组),GA浓度2.5%组(D组),各组与GA交联作用后经5-羧基荧光素琥珀酰亚胺酯(5-FAMSE)处理,然后在荧光显微镜下照相,照片进行荧光强度分析(应用专业图像分析软件--Image-Pro Plus 6.0),结果A组>B组>C组>D组,各组之间差异具有显著统计学意义(P<0.01)。各组同时取一定量血管组织经GA交联后提取其组织蛋白,进行传统的聚丙烯酰胺凝胶电泳(PGE)与5-FAMSE法进行对照,结果组织蛋白提取量A组>B组/C组/D组,无法定量比较B、C、D各组间差异。以上可说明5-FAMSE是一种有效的荧光试剂,可以清楚地反映小血管氨基残端的变化,可以更为方便且直观的评价经GA处理的小血管的交联程度。展开更多
文摘Through reducing incubation time,the low-level lactosed polyethyleneimine(L-PEI)was prepared by reductive amination of PEI with lactose.The content of galactose residue in the complex was determined with phenol-sulfuric acid method and the lactose rate was calculated.The result shows that:a good linear relationship within galactose concentration range of 1.25～12.5μg/mL(r=0.996),RSD of reappearance was 2.16%,the average rate of recoveries was 92.5%(RSD=2.18%).With a 6.27% lactose rate,the developed L-PEI reached its expected level.
文摘利用荧光技术创建一种新的方法评价经交联处理异体组织的交联程度。以新西兰兔胸主动脉作为研究对象,按与其作用的戊二醛(GA)浓度不同分为空白对照组--未与GA作用(A组),GA浓度0.625%组(B组),GA浓度1.25%组(C组),GA浓度2.5%组(D组),各组与GA交联作用后经5-羧基荧光素琥珀酰亚胺酯(5-FAMSE)处理,然后在荧光显微镜下照相,照片进行荧光强度分析(应用专业图像分析软件--Image-Pro Plus 6.0),结果A组>B组>C组>D组,各组之间差异具有显著统计学意义(P<0.01)。各组同时取一定量血管组织经GA交联后提取其组织蛋白,进行传统的聚丙烯酰胺凝胶电泳(PGE)与5-FAMSE法进行对照,结果组织蛋白提取量A组>B组/C组/D组,无法定量比较B、C、D各组间差异。以上可说明5-FAMSE是一种有效的荧光试剂,可以清楚地反映小血管氨基残端的变化,可以更为方便且直观的评价经GA处理的小血管的交联程度。
