This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering sca...This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture.展开更多
The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue enginee...The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue engineering. PVA was blended with various amounts of GAG and COL. Different proportional scaffolds could be obtained with different molecular weight and alcoholysis degree of PVA and different amounts of GAG,which exhibited high water content (60%-95%) and showed different inner configuration with swelling ratio (120%-620%). SEM proved that different composite materials had different porous structures.展开更多
The objective of this paper is to design a based on composite membrane with certain mechanical porous polyvinyl alcohol （PVA） strength and biocompatibilities serving as tissue regenerative scaffolds. PVA-glycosamino...The objective of this paper is to design a based on composite membrane with certain mechanical porous polyvinyl alcohol （PVA） strength and biocompatibilities serving as tissue regenerative scaffolds. PVA-glycosaminoglycan （GAG）-type I collagen （COL） composite membrane was fabricated by PVA with different molecular weight （Mw） and alcoholysis degree （AD） being blended with certain amounts of GAG and COL and dried at 38~C for 24 h. The water content of the composite membranes were from 61.9% to 95.1% and swelling ratio ranged from 123.6% to 621.7%. Scanning electron micro- scope （SEM） analysis proved that PVA-GAG-COL composite membrane has porous and homogenous structure. Biocompatibility test results showed that the composite membrane was nontoxic, which could promote adhesion and proliferation of fibroblasts on the com- posite membrane. In conclusion, PVA-GAG-COL composite membrane with high water content and swelling ratio, suitable mechanical strength and good biocompatibility, has potential in tissue engineering and regenerative medicine.展开更多
基金National High-tech Reasearch and Development Program of China(863 Program)grant number:2077AA09Z436+1 种基金Guangdong Project '211'grant number:50621030
文摘This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture.
文摘The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue engineering. PVA was blended with various amounts of GAG and COL. Different proportional scaffolds could be obtained with different molecular weight and alcoholysis degree of PVA and different amounts of GAG,which exhibited high water content (60%-95%) and showed different inner configuration with swelling ratio (120%-620%). SEM proved that different composite materials had different porous structures.
基金National Natural Science Foundation of China,grant number:51070853Guangzhou Technology Plans,grant number:2010B103315 and 2010J-E041
文摘The objective of this paper is to design a based on composite membrane with certain mechanical porous polyvinyl alcohol （PVA） strength and biocompatibilities serving as tissue regenerative scaffolds. PVA-glycosaminoglycan （GAG）-type I collagen （COL） composite membrane was fabricated by PVA with different molecular weight （Mw） and alcoholysis degree （AD） being blended with certain amounts of GAG and COL and dried at 38~C for 24 h. The water content of the composite membranes were from 61.9% to 95.1% and swelling ratio ranged from 123.6% to 621.7%. Scanning electron micro- scope （SEM） analysis proved that PVA-GAG-COL composite membrane has porous and homogenous structure. Biocompatibility test results showed that the composite membrane was nontoxic, which could promote adhesion and proliferation of fibroblasts on the com- posite membrane. In conclusion, PVA-GAG-COL composite membrane with high water content and swelling ratio, suitable mechanical strength and good biocompatibility, has potential in tissue engineering and regenerative medicine.
