The effect of temperature on the characteristic of Fourier Transform Infrared(FTIR),the dependence of the average molecular weight and the polydispersity on the annealing temperature and the relationship between linea...The effect of temperature on the characteristic of Fourier Transform Infrared(FTIR),the dependence of the average molecular weight and the polydispersity on the annealing temperature and the relationship between linear expansion coefficients and temperature of poly(propylene carbonate) end-capped with maleic anhydride(MA-PPC) were investigated.The vibration peak of hydroxyl group at 1714?cm -1 in the FTIR spectra,which belongs to the carboxyl end-group in MA-PPC,decreases with increasing the temperature of the sample and it disappeared totally when the sample was heated over 70℃.The average molecular weight of MA-PPC almost doubled when the sample was annealed at 110～150℃ for 30 min,however the polydispersity was maintained roughly.A new relaxation transition at about 80℃ was detected on the curve of the linear expansion coefficient versus temperature that is the characteristic of macromolecular coupling or crosslinking reaction.These results confirmed that the macromolecular coupling reaction was carried out between the end carboxyl groups of MA-PPC when the sample was heated over 70℃.展开更多
背景:聚丁二酸丁二醇酯、聚碳酸亚丙酯是近年来发展的新型医用材料,具有良好的生物相容性、可降解性及价格低廉等优点。目的:采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜,评价其理化性能、体外降解性能及对细胞增殖的影响...背景:聚丁二酸丁二醇酯、聚碳酸亚丙酯是近年来发展的新型医用材料,具有良好的生物相容性、可降解性及价格低廉等优点。目的:采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜,评价其理化性能、体外降解性能及对细胞增殖的影响。方法:采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜:常温下称取聚丁二酸丁二醇酯、聚碳酸亚丙酯各0.9 g,溶解于10 m L三氯甲烷中,磁力搅拌,待其充分溶解后,将纺丝液加入纺丝管内,调整纺丝距离约15 cm,最后开启电源将电压调至18 k V即可,扫描电镜观察其表面形态,检测其强度、接触角、吸水率及体外降解过程中的pH值和失重率。将人骨肉瘤细胞MG63与生物膜共培养7 d后,采用CCK-8检测细胞增殖。结果与结论:扫描电镜可见聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜呈均匀多孔状,孔孔交通,纤维直径约0.88μm,平均孔径约5.68μm,孔隙率为78.3%,断裂强度平均为2.31 MPa,断裂伸长率为23.48%,接触角约为87°,吸水率为68.54%;生物膜降解过程中pH值呈缓慢降低,第12周时pH值为6.76,同时该生物膜处于均匀缓慢的降解过程中,12周时失重率为6.04%。CCK-8实验结果显示该生物膜对细胞的增殖具有一定的促进作用。表明静电纺丝聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜具有良好的理化性能,具备良好的空间维持能力、湿润性及降解性,能为骨组织再生提供充足的时间。展开更多
文摘The effect of temperature on the characteristic of Fourier Transform Infrared(FTIR),the dependence of the average molecular weight and the polydispersity on the annealing temperature and the relationship between linear expansion coefficients and temperature of poly(propylene carbonate) end-capped with maleic anhydride(MA-PPC) were investigated.The vibration peak of hydroxyl group at 1714?cm -1 in the FTIR spectra,which belongs to the carboxyl end-group in MA-PPC,decreases with increasing the temperature of the sample and it disappeared totally when the sample was heated over 70℃.The average molecular weight of MA-PPC almost doubled when the sample was annealed at 110～150℃ for 30 min,however the polydispersity was maintained roughly.A new relaxation transition at about 80℃ was detected on the curve of the linear expansion coefficient versus temperature that is the characteristic of macromolecular coupling or crosslinking reaction.These results confirmed that the macromolecular coupling reaction was carried out between the end carboxyl groups of MA-PPC when the sample was heated over 70℃.
文摘背景:聚丁二酸丁二醇酯、聚碳酸亚丙酯是近年来发展的新型医用材料,具有良好的生物相容性、可降解性及价格低廉等优点。目的:采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜,评价其理化性能、体外降解性能及对细胞增殖的影响。方法:采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜:常温下称取聚丁二酸丁二醇酯、聚碳酸亚丙酯各0.9 g,溶解于10 m L三氯甲烷中,磁力搅拌,待其充分溶解后,将纺丝液加入纺丝管内,调整纺丝距离约15 cm,最后开启电源将电压调至18 k V即可,扫描电镜观察其表面形态,检测其强度、接触角、吸水率及体外降解过程中的pH值和失重率。将人骨肉瘤细胞MG63与生物膜共培养7 d后,采用CCK-8检测细胞增殖。结果与结论:扫描电镜可见聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜呈均匀多孔状,孔孔交通,纤维直径约0.88μm,平均孔径约5.68μm,孔隙率为78.3%,断裂强度平均为2.31 MPa,断裂伸长率为23.48%,接触角约为87°,吸水率为68.54%;生物膜降解过程中pH值呈缓慢降低,第12周时pH值为6.76,同时该生物膜处于均匀缓慢的降解过程中,12周时失重率为6.04%。CCK-8实验结果显示该生物膜对细胞的增殖具有一定的促进作用。表明静电纺丝聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜具有良好的理化性能,具备良好的空间维持能力、湿润性及降解性,能为骨组织再生提供充足的时间。
