目的评价光接枝改性对聚羟基丁酸-羟基异戊酯(copolymers of 3-hydroxybutyrate and 3-hy-droxyvalerate,PHBV)与绵羊BMSCs相容性的影响。方法取6月龄雄性绵羊1只,体重17kg,髂后棘穿刺抽取骨髓,全骨髓贴壁培养法分离培养BMSCs。制备PHB...目的评价光接枝改性对聚羟基丁酸-羟基异戊酯(copolymers of 3-hydroxybutyrate and 3-hy-droxyvalerate,PHBV)与绵羊BMSCs相容性的影响。方法取6月龄雄性绵羊1只,体重17kg,髂后棘穿刺抽取骨髓,全骨髓贴壁培养法分离培养BMSCs。制备PHBV膜、光接枝PHBV膜、PHBV支架及光接枝PHBV支架。取第3代BMSCs以1×105/mL接种至培养板,分别与PHBV膜和光接枝PHBV膜复合培养,接种后1、2、6h计算细胞在两种膜上的黏附率;另将BMSCs以1×104/孔接种于两种膜上,接种后6h、3d及1、3周取材,扫描电镜观察细胞在两种膜上的生长情况;另将BMSCs以2×105/孔接种于两种支架上,接种后3d及1、3周取材,扫描电镜观察细胞在两种支架上的生长情况;再将BMSCs以2×104/孔接种于两种膜上,5d后收集细胞,流式细胞仪分析细胞周期;再将BMSCs以1×107/孔接种于两种支架上,接种后4、8、12d测定细胞内蛋白质和DNA含量。结果接种后1h,PHBV膜上的细胞黏附率为37.5%±5.3%,光接枝PHBV膜为52.7%±6.0%,二者比较差异有统计学意义(P<0.05);接种后2、6h,两种支架上的细胞黏附率比较差异无统计学意义(P>0.05)。扫描电镜观察:光接枝PHBV膜以及光接枝PHBV支架上的细胞黏附较早,接种后1周两种材料上的细胞数较多,且细胞分泌物亦较多。流式细胞仪检测见两种膜上的BMSCs均为正常二倍体细胞,未见异倍体细胞,细胞周期和增殖指数比较差异均无统计学意义(P>0.05)。BMSCs接种至两种支架上4、8、12d,细胞内DNA、蛋白质含量比较差异均无统计学意义(P>0.05)。结论光接枝表面改性增强了PHBV对绵羊BMSCs的早期吸附,改善了其生物相容性。展开更多
A composite material was fabricated by applying a biodegradable drug delivery coating,consisting of poly（3-hydroxyburyrate-co-3-hydroxyvalerate）（PHBV） and icariin,to an anodic oxidized titanium plate.The coating w...A composite material was fabricated by applying a biodegradable drug delivery coating,consisting of poly（3-hydroxyburyrate-co-3-hydroxyvalerate）（PHBV） and icariin,to an anodic oxidized titanium plate.The coating was prepared by evaporating chloroform solution containing PHBV and icariin on the titanium plate under vacuum condition.Icariin/PHBV coated titanium plates significantly enhance the proliferation of MG-63 cells compared with the PHBV coated and anodic oxidized ones.Increased icariin contained in the coating displays an elevated influence on cell proliferation.The results show that icariin gradually releases from the coating to cells mainly through the phospholipid-based cellular membrane instead of the culture medium.The overall results suggest that the novel icariin/PHBV coating can be used to enhance the bioactivity of titanium based orthopedic implants.展开更多
Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilic montmorillonite (PHBV/OMMT) nanocomposites were prepared and the biodegradability of the PHBV/OMMT nanocomposites was studied by a cultivation degrading metho...Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilic montmorillonite (PHBV/OMMT) nanocomposites were prepared and the biodegradability of the PHBV/OMMT nanocomposites was studied by a cultivation degrading method in soil suspension. The relationship between structure and biodegradability of PHBV/OMMT nanocomposites was investigated. The results showed that the biodegradability of PHBV/OMMT nanocomposites decreased with increasing amount of OMMT and it was related to the number of PHBV degrading microorganisms in degradation environment, the anti-microbial property of OMMT and the degree of crystallinity of the nanocomposites.展开更多
文摘目的评价光接枝改性对聚羟基丁酸-羟基异戊酯(copolymers of 3-hydroxybutyrate and 3-hy-droxyvalerate,PHBV)与绵羊BMSCs相容性的影响。方法取6月龄雄性绵羊1只,体重17kg,髂后棘穿刺抽取骨髓,全骨髓贴壁培养法分离培养BMSCs。制备PHBV膜、光接枝PHBV膜、PHBV支架及光接枝PHBV支架。取第3代BMSCs以1×105/mL接种至培养板,分别与PHBV膜和光接枝PHBV膜复合培养,接种后1、2、6h计算细胞在两种膜上的黏附率;另将BMSCs以1×104/孔接种于两种膜上,接种后6h、3d及1、3周取材,扫描电镜观察细胞在两种膜上的生长情况;另将BMSCs以2×105/孔接种于两种支架上,接种后3d及1、3周取材,扫描电镜观察细胞在两种支架上的生长情况;再将BMSCs以2×104/孔接种于两种膜上,5d后收集细胞,流式细胞仪分析细胞周期;再将BMSCs以1×107/孔接种于两种支架上,接种后4、8、12d测定细胞内蛋白质和DNA含量。结果接种后1h,PHBV膜上的细胞黏附率为37.5%±5.3%,光接枝PHBV膜为52.7%±6.0%,二者比较差异有统计学意义(P<0.05);接种后2、6h,两种支架上的细胞黏附率比较差异无统计学意义(P>0.05)。扫描电镜观察:光接枝PHBV膜以及光接枝PHBV支架上的细胞黏附较早,接种后1周两种材料上的细胞数较多,且细胞分泌物亦较多。流式细胞仪检测见两种膜上的BMSCs均为正常二倍体细胞,未见异倍体细胞,细胞周期和增殖指数比较差异均无统计学意义(P>0.05)。BMSCs接种至两种支架上4、8、12d,细胞内DNA、蛋白质含量比较差异均无统计学意义(P>0.05)。结论光接枝表面改性增强了PHBV对绵羊BMSCs的早期吸附,改善了其生物相容性。
基金Project (2010DFA32270) supported by International Science & Technology Cooperation Program of ChinaProject (2010) supported by Scientific Research Foundation for the Returned Oversea Scholars of Ministry of Education of China
文摘A composite material was fabricated by applying a biodegradable drug delivery coating,consisting of poly（3-hydroxyburyrate-co-3-hydroxyvalerate）（PHBV） and icariin,to an anodic oxidized titanium plate.The coating was prepared by evaporating chloroform solution containing PHBV and icariin on the titanium plate under vacuum condition.Icariin/PHBV coated titanium plates significantly enhance the proliferation of MG-63 cells compared with the PHBV coated and anodic oxidized ones.Increased icariin contained in the coating displays an elevated influence on cell proliferation.The results show that icariin gradually releases from the coating to cells mainly through the phospholipid-based cellular membrane instead of the culture medium.The overall results suggest that the novel icariin/PHBV coating can be used to enhance the bioactivity of titanium based orthopedic implants.
基金The National Natural Science Foundation of China (No. 20374032) Chinese Education Ministry Foundation for N & T Joint Academy
文摘Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilic montmorillonite (PHBV/OMMT) nanocomposites were prepared and the biodegradability of the PHBV/OMMT nanocomposites was studied by a cultivation degrading method in soil suspension. The relationship between structure and biodegradability of PHBV/OMMT nanocomposites was investigated. The results showed that the biodegradability of PHBV/OMMT nanocomposites decreased with increasing amount of OMMT and it was related to the number of PHBV degrading microorganisms in degradation environment, the anti-microbial property of OMMT and the degree of crystallinity of the nanocomposites.
