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聚胺酸修饰酵母对碱性品红和亚甲基蓝的吸附行为 被引量:3
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作者 许彬彬 佟蜜 +1 位作者 孙小梅 李步海 《化学工程师》 CAS 2008年第6期60-62,共3页
用面包酵母作单体、戊二醛为变联剂,制备成交联酵母后用聚胺酸进行修饰,得到聚胺酸修饰酵母。研究了pH值、染料浓度、吸附时间、温度对聚胺酸修饰酵母吸附能力的影响。结果表明,聚胺酸修饰面包酵母可同时吸附碱性品红和亚甲基蓝。在p... 用面包酵母作单体、戊二醛为变联剂,制备成交联酵母后用聚胺酸进行修饰,得到聚胺酸修饰酵母。研究了pH值、染料浓度、吸附时间、温度对聚胺酸修饰酵母吸附能力的影响。结果表明,聚胺酸修饰面包酵母可同时吸附碱性品红和亚甲基蓝。在pH值为4.0~11.0,酸度对吸附量的影响较小,吸附反应分别在40、20min达到平衡,过程符合Langmuir模型,最大吸附量分别为335.9、694.2mg·g^-1。 展开更多
关键词 酵母 聚胺酸 碱性品红 亚甲基蓝 吸附
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Effective adsorption of sulfate ions with poly(m-phenylenediamine) in aqueous solution and its adsorption mechanism 被引量:5
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作者 桑培伦 王云燕 +2 位作者 张理源 柴立元 王海鹰 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第1期243-252,共10页
Sulfate adsorption by poly(m-phenylenediamine)s(PmPDs) with various oxidation states synthesized through chemically oxidative polymerization was investigated.Series of sorption experiments were conducted,and the a... Sulfate adsorption by poly(m-phenylenediamine)s(PmPDs) with various oxidation states synthesized through chemically oxidative polymerization was investigated.Series of sorption experiments were conducted,and the adsorption mechanism and the relationship between oxidation state and adsorption performance were studied with the characterization of Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS),pH tracking and energy calculation.The results show that the adsorption performance in acidic solution is improved with the decrease of oxidation state of poly(m-phenylenediamine)(PmPD).The rate constant is as high as 425.5 mg/(g·min) in the short equilibrium time of 30 min.The estimated highest adsorptivity of sulfate ions is 95.1%.According to the Langmuir equation,the adsorbance is 108.5 mg/g.The sulfate desorption efficiency is about 95% and the accumulative adsorbance is up to 487.95 mg/g in 5 cycles. 展开更多
关键词 poly(m-phenylenediamine) sulfate ions ADSORPTION adsorption rate oxidation state desorption efficiency
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胶料加温搅拌真空脱泡装置 被引量:1
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作者 尹光岩 《生产技术与工艺管理》 2002年第2期19-19,共1页
关键词 聚胺酸 胶料 加温搅拌 真空脱泡装置
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Preparation of Hollow Silica Microspheres via Poly(N-isopropylacrylamide) 被引量:2
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作者 汪谟贞 陈宇晨 +1 位作者 葛学平 葛学武 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2012年第1期120-124,I0004,共6页
Core-shell structured SiO2/poly(N-isopropylacrylamide) (SiO2/PNIPAM) microspheres were successfully fabricated through hydrolysis and condensation reaction of tertraethyl orthosilicate (TEOS) on the surface of P... Core-shell structured SiO2/poly(N-isopropylacrylamide) (SiO2/PNIPAM) microspheres were successfully fabricated through hydrolysis and condensation reaction of tertraethyl orthosilicate (TEOS) on the surface of PNIPAM template at 50 ~C. The PNIPAM template can be easily removed by water at room temperature so that SiO2 hollow microspheres were finally obtained. The transmission electron microscope and scanning electron microscope observations indicated that SiO2 hollow microspheres with an average diameter of 150 nm can be formed only if there are enough concentration of PNIPAM and TEOS, and the hy- drolysis time of TEOS. FTIR analysis showed that part of PNIPAM remained on the wall of SiO2 because of the strong interaction between PNIPAM and silica. This work provides a clean and efficient way to prepare hollow microspheres. 展开更多
关键词 SiO2 hollow microsphere Poly(N-isopropylacrylamide) Tertraethyl orthosilicate Soft template
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Synthesis of poly(m-phenylenediamine) with improved properties and superior prospect for Cr(VI) removal 被引量:2
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作者 于婉婷 柴立元 +1 位作者 张理源 王海鹰 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第11期3490-3498,共9页
Chemically oxidative polymerization of m-phenylenediamine was improved through adding the weak alkaline, Na2CO3. Results show that the poly (m-phenylenediamine) (PmPD) possesses a weak solubility in acidic solutio... Chemically oxidative polymerization of m-phenylenediamine was improved through adding the weak alkaline, Na2CO3. Results show that the poly (m-phenylenediamine) (PmPD) possesses a weak solubility in acidic solution according to total organic carbon (TOC) that the TOC is less than 8 mg/L, which is much lower than the discharge standard (20 mg/L). The TOC of the PmPD synthesized with NaOH can be as high as 120.9 mg/L. This very weak solubility of PmPD synthesized with Na2CO3 facilitates its application in water purification. The oxidation state of PmPD is decreased and the yield is increased with a maximum of 84%, promoting the concentration of Na2CO3 in the synthesis. Moreover, the Cr(VI) performance of PmPD was marvelously enhanced with Na2CO3 to improve the synthesis. The largest Cr(VI) adsorbance can reach as high as 666.8 mg/g, which is far more than the performance of other common adsorbents. 展开更多
关键词 poly (m-phenylenediamine) chromium (VI) sodium carbonate adsorption
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Preparation and characterization of poly(amic acid)-stabilized silver nanoparticles 被引量:1
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作者 李俊 方悦诚 +1 位作者 贺国文 李衡峰 《Journal of Central South University》 SCIE EI CAS 2013年第6期1475-1481,共7页
Stable and monodispersed silver nanoparticles were produced through a mild,convenient,one-pot method based on the reduction of silver nitrate in the presence of poly(amic acid) (PAA) as a stabilizer.The surface plasma... Stable and monodispersed silver nanoparticles were produced through a mild,convenient,one-pot method based on the reduction of silver nitrate in the presence of poly(amic acid) (PAA) as a stabilizer.The surface plasma band transition was monitored along with time in the reaction mixture for three sets of experiments by ultraviolet-visible spectroscopy.Analysis of the data with the Avrami equation yielded n exponent with values between 0.5 and 1.5,demonstrating three-dimensional heterogeneous nucleation and diffusion-controlled growth,accompanied by soft impingement effect.XRD and TEM analyses show a softly agglomerated polycrystalline state and a nearly spherical morphology (<50 nm) of nanoparticles.The FT-IR result indicates that the PAA molecular structure could be hardly influenced by the formation of nanoparticles. 展开更多
关键词 silver nanoparticles poly(amic acid) ultraviolet-visible spectroscopy crystallization kinetics
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Critical roles of molybdate anions in enhancing capacitive and oxygen evolution behaviors of LDH@PANI nanohybrids 被引量:2
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作者 Qiang Hu Hua Wang +7 位作者 Feifei Xiang Qiaoji Zheng Xinguo Ma Yu Huo Fengyu Xie Chenggang Xu Dunmin Lin Jisong Hu 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2021年第6期980-993,共14页
Low-overpotential layered hydroxides(LDHs)with high theoretical capacity are promising electrodes for supercapaterry and oxygen evolution reaction;however,the low electronic conductivity and insufficient active sites ... Low-overpotential layered hydroxides(LDHs)with high theoretical capacity are promising electrodes for supercapaterry and oxygen evolution reaction;however,the low electronic conductivity and insufficient active sites of bulk LDHs increase the internal resistance and reduce the capacity and oxygen-production efficiency of electrodes.Herein,we prepared a polyaniline-coated Ni-Co-layered double hydroxide intercalated with MoO_(4)^(2−)(M-LDH@PANI)composite electrode using a two-step method.As the amount of MoO_(4)^(2−)in the LDH increases,acicular microspheres steadily evolve into flaky microspheres with a high surface area,providing more active electrochemical sites.Moreover,the amorphous PANI coating of M-LDH boosts the electronic conductivity of the composite electrode.Accordingly,the M-LDH@PANI at an appropriate level of MoO_(4)^(2−)exhibits significantly enhanced energy storage and catalytic performance.Experimental analyses and theoretical calculations reveal that a small amount of MoO_(4)^(2−)is conducive to the expansion of LDH interlayer spacing,while an excessive amount of MoO_(4)^(2−)combines with the H atoms of LDH,thus competing with OH^(−),resulting in reduced electrochemical performance.Moreover,M-LDH flaky microspheres can efficiently modulate deprotonation energy,greatly accelerating surface redox reactions.This study provides an explanation for an unconventional mechanism,and a method for the modification of LDH-based materials for anion intercalation. 展开更多
关键词 Layered hydroxide LDH PANI MoO_(4)^(2−) Intercalated hierarchical structures Supercapaterry Electrocatalyst
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Synthesis and characterization of PMoV/Fe_3O_4/g-C_3N_4 from melamine:An industrial green nanocatalyst for deep oxidative desulfurization 被引量:2
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作者 Ezzat Rafiee Maryam Khodayari 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2017年第3期458-468,共11页
A facile approach to the preparation of a novel magnetically separable H_5PMo_(10)V_2O_(40)/Fe_3O_4/g-C_3N_4(PMoV/Fe_3O_4/g-C_3N_4) nanocomposite by chemical impregnation is demonstrated.The prepared nanocomposi... A facile approach to the preparation of a novel magnetically separable H_5PMo_(10)V_2O_(40)/Fe_3O_4/g-C_3N_4(PMoV/Fe_3O_4/g-C_3N_4) nanocomposite by chemical impregnation is demonstrated.The prepared nanocomposite was characterized and its acidity was measured by potentiometric titration.PMoV/Fe_3O_4/g-C_3N_4 showed high catalytic activity in the selective oxidative desulfurization of sulfides to their corresponding sulfoxides or sulfones.The catalytic oxidation of a dibenzothiophene(DBT)-containing model oil and that of real oil were also studied under optimized conditions.In addition,the effects of various nitrogen compounds,as well as the use of one- and two-ring aromatic hydrocarbons as co-solvents,on the catalytic removal of sulfur from DBT were investigated.The catalyst was easily separated and could be recovered from the reaction mixture by using an external magnetic field.Additionally,the remaining reactants could be separated from the products by simple decantation if an appropriate solvent was chosen for the extraction.The advantages of this nanocatalyst are its high catalytic activity and reusability;it can be used at least four times without considerable loss of activity. 展开更多
关键词 Graphitic carbon nitride NANOCOMPOSITE MELAMINE Heteropoly acid Oxidative desulfurization OIL
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Polyaniline Nanotubes Prepared by One-Step Synergistic Polymerization of Aniline and Acrylic Acid 被引量:1
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作者 Han-hong Huang Yi-hu Wu +1 位作者 Mo-zhen Wang Xue-wu Ge 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2018年第6期827-832,734,共7页
The electrochemical property of electrode materials greatly depends on their morphologies. This report introduces a novel and facile synthesis method for polyaniline (PANI) nanotubes from one-step synergistic polymeri... The electrochemical property of electrode materials greatly depends on their morphologies. This report introduces a novel and facile synthesis method for polyaniline (PANI) nanotubes from one-step synergistic polymerization of aniline and acrylic acid in an aqueous solution induced by the addition of ammonium persulfate (APS). The molar ratio of aniline to AA (X{ani/AA}) is found to have great in fluence on the morphology of the produced PANI. Hollow PANI nanotubes with an average inner diameter of 80 nm and outer diameter of 180 nm can be mainly produced when X{ani/AA} is not higher than 1. The electrochemical properties of the prepared PANI nanotubes have been investigated using a three-electrode system. The specific capacitance of PANI nanotubes can reach 436 F/g at a current density of 0.5 A/g in 1 mol/L H2SO4 solution. Furthermore, the specific capacitance of the PANI nanotube maintains 89.2% after 500 charging/discharging cycles at a current density of 0.5 A/g, indicating a good cycling stability. 展开更多
关键词 Polyaniline nanotubes Synergistic polymerization Acrylic acid SUPERCAPACITOR
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Research on Sex and Tissue Differences in Isozymic Phenotype of Varicorhinus macrolepis through Isozyme Electrophoresis 被引量:6
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作者 庞秋香 庞书香 +5 位作者 赵博生 宿烽 孙欢欢 颜越 吕志林 刘龙 《Agricultural Science & Technology》 CAS 2010年第5期101-105,共5页
Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C. 1.1.1.37) isozymes zymogram in 11 different types of tissues of male... Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C. 1.1.1.37) isozymes zymogram in 11 different types of tissues of male and female Varicorhinus macrolepis. It had been found for the first time that the phenotype of malate dehydrogenase (MDH),acid phosphatase (ACP) and superoxide dismutase (SOD) showed difference between male and female V. macrolepis,and there was no difference among different individuals in the same sex. Therefore,the electrophoresis band of malate dehydrogenase,acid phosphatase and superoxide dismutase could be used as an indicator for the identification of gender and tissues of V. macrolepis,which would provide basic data for the developmental genetics,variety improvement and directed breeding of V. macrolepis groups,thus facilitating the development and protection of this valuable fish species. 展开更多
关键词 Varicorhinus macrolepis Malate dehydrogenase Acid phosphatase Superoxide dismutase Polyacrylamide gel electrophoresis (PAGE)
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Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity Partitioning 被引量:3
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作者 林东强 姚善泾 +1 位作者 梅乐和 朱自强 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2000年第4期310-314,共5页
The synthesis route was investigated and optimized for the preparation of iminodiacetic acid-polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in aqueous two-phase systems. IDA-PEG was synt... The synthesis route was investigated and optimized for the preparation of iminodiacetic acid-polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in aqueous two-phase systems. IDA-PEG was synthesized from PEG in two steps by the reaction of iminodiacetic acid with a monosubstituted derivative of epichlorohydrin-activated PEG. The Cu2+ content combined with IDA-PEG was determined by atomic absorption spectrometry as 0.5 mol·mol^-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase in polyethylene glycol/hydroxypropyl starch aqueous two-phase systems was studied to clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG. 展开更多
关键词 immobilized metal ion affinity partitioning iminodiacetic acid-polyethylene glycol aqueous two-phase system
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Swelling Behaviors of Polyaniline-Poly(Acrylic Acid) Hydrogels
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作者 张幼维 赵炯心 +2 位作者 厉啸峰 陶勇 吴承训 《Journal of Donghua University(English Edition)》 EI CAS 2005年第2期100-104,共5页
Using poly(acrylic acid) (PAA) aqueous solution, NaOH aqueous solution, aniline (An) and ammonium persulfate (APS), PAn-PAA hydrogels with a semi-interpenetrating structure connected by physical interlocks, ch... Using poly(acrylic acid) (PAA) aqueous solution, NaOH aqueous solution, aniline (An) and ammonium persulfate (APS), PAn-PAA hydrogels with a semi-interpenetrating structure connected by physical interlocks, chemical ion bonds and hydrogen bonds were prepared. The swelling properties of the hydrogels in solutions of different pH values (adjusted by adding NaOH or HCI) were studied. All the hydrogels prepared have similar swelling curves (the curves of equilibrium swelling ratio vs. pH value) and reach their maximum swelling at pH of 8 - 10. The maximum swelling ratio of the hydrogels is dependent on composition, including molecular weight of PAA, polymer content of the hydrogel, and molar ratios of AA to An, APS to An, and NaOH to AA, And the compositional dependence of the swelling capacity of PAn-PAA hydrogels was also studied. 展开更多
关键词 Poly (acrylic acid) POLYANILINE HYDROGEL Semi-Interpenetrating SWELLING
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Preparation , Structure and DC Conductivity of Organic Semiconductor PA NI-C_4H_6O_6
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作者 AdulkaderM.Elamin YAOKai-lun 《Semiconductor Photonics and Technology》 CAS 1999年第3期166-170,共5页
A new organic semiconductor tartaric acid doped salt of emeraldine polyaniline (PANI-C 4H 6O 6) has been obtained by the method of oxidative polymerization of monomeric aniline with ammonium persulfate in acidic solut... A new organic semiconductor tartaric acid doped salt of emeraldine polyaniline (PANI-C 4H 6O 6) has been obtained by the method of oxidative polymerization of monomeric aniline with ammonium persulfate in acidic solution. The structure was characterized by Fourier Transform Infrared technique (FTIR) and X-ray diffraction (XRD). The temperature dependence dc conductivity δ dc ( T ) shows a semiconductor behavior and follows the quasi one dimensional variable range hopping (Q1D-VRH) model. Data on δ dc ( T ) are also discussed. 展开更多
关键词 DC Conductivity Organic Semiconductor Polyaniline Structure Tartaric Acid CLC number:O631.23 O632.7 TN304.52 Document code:A
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Electrocatalytic oxidation behavior of L-cysteine at Pt microparticles modified nanofibrous polyaniline film electrode
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作者 马淞江 罗胜联 +2 位作者 周海晖 旷亚非 宁晓辉 《Journal of Central South University of Technology》 EI 2008年第2期170-175,共6页
Platinum(Pt)/nanofibrous polyaniline(PANI) electrode was prepared by pulse galvanostatic method and characterized by scanning electron microscopy.The electrochemical behavior of L-cysteine at the Pt/nanofibrous PANI e... Platinum(Pt)/nanofibrous polyaniline(PANI) electrode was prepared by pulse galvanostatic method and characterized by scanning electron microscopy.The electrochemical behavior of L-cysteine at the Pt/nanofibrous PANI electrode was investigated by cyclic voltammetry.The results indicate that the pH value of the solution and the Pt loading of the electrode have great effect on the electrocatalytic property of the Pt /nanofibrous PANI electrode;the suitable Pt loading of the electrode is 600 μg/cm2 and the suitable pH value of the solution is 4.5 for investigating L-cysteine oxidation.The L-cysteine sensor based on the Pt/nanofibrous PANI electrode has a good selectivity,reproducibility and stability.The Pt/nanofibrous PANI electrode is highly sensitive to L-cysteine,and the linear calibration curve for the oxidation of L-cysteine can be observed in the range of 0.2-5.0 mmol/L. 展开更多
关键词 electrocatalytic oxidation electrode POLYANILINE platinum L-CYSTEINE NANOFIBRE
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Iron(Ⅱ) tetrasulfophthalocyanine mimetic enzymatic synthesis of conducting polyaniline in micellar system 被引量:2
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作者 胡兴 刘惠 邹国林 《Journal of Central South University》 SCIE EI CAS 2009年第5期743-748,共6页
Iron(Ⅱ) tetrasulfophthalocyanine(FeTSPc) ,as a novel mimetic enzyme of peroxidase,was used in the synthesis of a conducting polyaniline(PANI) /sodium dodecylsulfate(SDS) complex in SDS aqueous micellar solutions. The... Iron(Ⅱ) tetrasulfophthalocyanine(FeTSPc) ,as a novel mimetic enzyme of peroxidase,was used in the synthesis of a conducting polyaniline(PANI) /sodium dodecylsulfate(SDS) complex in SDS aqueous micellar solutions. The effects of pH,concentrations of aniline,SDS and H2O2,and reaction time on polymerization of aniline were studied in this case as shown by UV-Vis absorption spectroscopy. The results show that a wide range of pH(0.5-4.0) is required to produce the conducting PANI,and the optimal pH is 1.0 in SDS micelle. The optimal concentrations of aniline,SDS and H2O2 in feed,and reaction time in this case for the production of conducting PANI are respectively 10 mmol/L,10 mmol/L,25 mmol/L,and 15 h. FT-IR spectrum,elemental analysis,conductivity,cyclic voltammetry and thermogravimetric analysis confirm the thermal stability and electroactive form of PANI. 展开更多
关键词 iron( tetrasulfophthalocyanine POLYANILINE mimetic enzyme CONDUCTIVITY MICELLE
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Purification,Characterization and in vitro Anthelmintic Activity of a Neutral Metalloprotease from Laccocephalum mylittae 被引量:4
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作者 周立华 许勤勤 +3 位作者 张一琼 周振兴 管文军 李永泉 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第1期122-128,共7页
A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold pur... A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold purification and a final yield of 21.76%.The subunit molecular weight of the protease is about 40000 estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The optimum reaction pH and temperature are 7.5 and 50oC,respectively.The protease activity is largely enhanced by Ca2 +,but highly inhibited by tetrasodium ethylenediaminetetraacetate(EDTA),a metal-chelator,suggesting that the enzyme is a metalloprotease.The Michaelis-Menten constan Km and Vmax value for casein substrate are 6.09 mg·ml -1and 21.32μg·min -1·ml -1, respectively.In vitro anthelmintic tests of the protease exhibit distinct lethal effects on the third stage larvae(L3)of Ascaris suum.Scanning electron microscopy and SDS-PAGE analysis indicates that the proteolysis of larvae proteins caused by this protease may relate to the anthelmintic activity of L.mylittae. 展开更多
关键词 METALLOPROTEASE Laccocephalum mylittae PURIFICATION CHARACTERIZATION anthelmintic activity
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Charge transport in conducting polyaniline co-doped with sulfosalicylic acid and dodecylbenzoyl sulfonic acid
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作者 马利 《Journal of Chongqing University》 CAS 2009年第3期170-174,共5页
We prepared conducting polyaniline (PAn) co-doped with sulfosalicylic acid (SSA) and dodecylbenzoyl sultonic acid (DBSA) in micro-emulsive polymerization, and studied its charge transport behaviors based on the ... We prepared conducting polyaniline (PAn) co-doped with sulfosalicylic acid (SSA) and dodecylbenzoyl sultonic acid (DBSA) in micro-emulsive polymerization, and studied its charge transport behaviors based on the measurement of its electrical conductivity in the temperature range between 203 K and 298 K. The conductivity was found to increase with temperature, similar to the case in semiconductors. Analyzing the experimental data with three models, namely the charge-energy-limitedtunneling model, Kivelson model and the three-dimensional variable range hopping (3D-VRH) model demonstrated that these models all describe well the charge transport behaviors of PAn co-doped with SSA and DBSA within the mentioned temperature range. From calculation with the 3D-VRH model, the hopping distance of the conducting PAn is obviously larger than its localization length. The PAn doped with SSA and DBSA enjoys desirable crystallinity due to the co-doping of two functional sulfonic acids. The macroscopic conductivity may correspond to three-dimensional transport in the network of the bundles, and the metallic islands may be attributed to quasi-one-dimensional bundles. 展开更多
关键词 conducting polymer conductive materials DOPING POLYANILINE charge transport variable range hopping
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IMMOBILIZATION OF Saccharomyces Cerevisiae USING POLY(ACRYLAMIDE)GEL FOR ASYMMETRIC SYNTHESIS OF R(-)-MANDELIC ACID
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作者 LI Zhongqin GUO Daiping +2 位作者 HUANG Xinghua YANG Kai XU Xiaoping 《Chinese Journal of Reactive Polymers》 2006年第1期81-85,共5页
In this paper,the poly(acrylamide)hydrogel used to immobilize saccharomyces cerevisiae for asymmetric synthesis of R(-)-mandelic acid was prepared with free radical ploymerization in deionized water at room temperatur... In this paper,the poly(acrylamide)hydrogel used to immobilize saccharomyces cerevisiae for asymmetric synthesis of R(-)-mandelic acid was prepared with free radical ploymerization in deionized water at room temperature under nitrogen atmosphere.The influence of the composition of hydrogel,loading amount of cells and culture conditions on the asymmetric synthesis was investigated.Results show that PAAm hydrogel is a feasible carrier for immobilization of cells which is a potential alternative method to prepare enantiomerically pure R(-)-mandelic acid. 展开更多
关键词 Poly(acrylamide) gel IMMOBILIZATION R(-)-mandelic acid Saccharomyces Cerevisiae.
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Novel biosensor-based microarray assay for detecting rs8099917 and rs12979860 genotypes 被引量:2
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作者 Pei-Yuan Li Xiao-Jun Zhou +3 位作者 Lan Yao Xin-Hua Fang Jiang-Nan Ren Jia-Wu Song 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第44期6481-6488,共8页
AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 genotypes.METHODS:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control... AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 genotypes.METHODS:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control probes were designed,constructed and arrayed on an optical biosensor to develop a microarray assay.Two sets of primers were used in a one tube polymerase chain reaction(PCR) system to amplify two target fragments simultaneously.The biosensor microarray contained probes that had been sequenced to confirm that they included the rs8099917C/T or rs12979860G/T alleles of interest and could serve as the specific assay standards.In addition to rehybridization of four probes of known sequence,a total of 40 clinical samples collected from hepatitis C seropositive patients were also tested.The target fragments of all 40 samples were amplified in a 50 μL PCR system.Ten μL of each amplicon was tested by BBM assay,and another 40 μL was used for sequencing.The agreement of the results obtained by the two methods was tested statistically using the kappa coefficient.The sensitivity of the BBM assay was evaluated using serial dilutions of ten clinical blood samples containing 10 3-10 4 white cells/μL.RESULTS:As shown by polyacrylamide gel electrophoresis,two target segments of the interleukin 28Bassociated polymorphisms(SNPs) were successfully amplified in the one-tube PCR system.The lengths of the two amplified fragments were consistent with the known length of the target sequences,137 and 159 bps.After hybridization of the PCR amplicons with the probes located on the BBM array,the signals of each allele of both the rs8099917 SNPs and rs12979860 SNPs were observed simultaneously and were clearly visible by the unaided eye.The signals were distinct from each other,could be interpreted visually,and accurately recorded using an ordinary digital camera.To evaluate the specificity of the assay,both the plasmids and clinical samples were applied to the microarray.First,30 PCR amplicons of the various SNP alleles were hybridized on the BBM microarray.Full agreement between plasmids and the BBM assay was observed,with 30/30 correct matches(100%).The kappa value for the BBM assay with plasmids was 1.00(P < 0.05).For the 40 clinical blood samples,the BBM assay hybridization and direct sequencing results were compared for each amplicon.For patient blood samples,agreement was 28/28 for rs8099917T/T,9/11 for rs8099917T/G,1/1 for rs8099917G/G,24/24 for rs12979860C/C,11/14 for rs12979860C/T,and 2/2 for rs12979860T/T.Only five clinical samples of amplicon assay and direct sequencing results were discordant and heterozygotes:2/11 rs8099917T/G and 3/14 rs12979860C/T.The agreement of outcomes between BBM assay and direct sequencing for the detection of rs8099917 and rs12979860 was 95% and 92.5%,respectively;and the corresponding kappa values were 0.88 and 0.85(A kappa value > 0.75 was defined as substantial agreement).The BBM assay and sequencing had similar specificities for detection and identification of the two SNPs and their alleles.The sensitivity evaluation showed that the BBM assay could detect and identify SNP sequences present in blood samples containing as few as 10 2 white blood cells/μL.CONCLUSION:This biosensor microarray assay was highly specific,sensitive,rapid and easy to perform.It is compatible with clinical practice for detection of rs8099917 and rs12979860. 展开更多
关键词 Biosensor-based microarray Hepatitis C vi-rus rs8099917 rs12979860 Detection ASSAY
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Pilot study on binding of bovine salivary proteins to grit silicates and plant phytoliths
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作者 Marcus MAU Thomas M.KAISER Karl-Heinz SüDEKUM 《Zoological Research》 CAS CSCD 北大核心 2013年第3期I0013-I0018,共6页
Mostly fed with grass in fresh or conserved form, cattle and other livestock have to cope with silicate defence bodies from plants (phytoliths) and environmental silicates (grit), which abrade tooth enamel and cou... Mostly fed with grass in fresh or conserved form, cattle and other livestock have to cope with silicate defence bodies from plants (phytoliths) and environmental silicates (grit), which abrade tooth enamel and could additionally interact with various salivary proteins. To detect potential candidates for silicate-binding proteins, bovine whole saliva was incubated with grass-derived phytoliths and silicates. Interactions of salivary proteins with pulverized bovine dental enamel and dentine were additionally analysed. After intense washing, the powder fractions were loaded onto 1D-polyacrylamide gels, most prominent adhesive protein bands were cut out and proteins were identified by mass spectrometry within three independent replicates. All materials were mainly botmd by bovine odorant-binding protein, bovine salivary protein 30× 10^3 and carbonic anhydrase VI. The phytolith/silicate fraction showed additional stronger interaction with haemoglobin β and lactoperoxidase. Conceivably, the binding of these proteins to the surfaces may contribute to biological processes occurring on them. 展开更多
关键词 Bovine salivary proteins Mass spectrometry PHYTOLITHS SILICATES Tooth enamel
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