AIM: To determine the plasma carnitine ester profile in adult patients with ulcerative culitis (UC) and compared with healthy control subjects. METHOD: Using ESI triple quadrupole tandem mass spectrometry, the car...AIM: To determine the plasma carnitine ester profile in adult patients with ulcerative culitis (UC) and compared with healthy control subjects. METHOD: Using ESI triple quadrupole tandem mass spectrometry, the carnitine ester profile was measured in 44 patients with UC and 44 age- and sex-matched healthy controls. RESULTS: There was no significant difference in the fasting free carnitine level between the patients with UC and the healthy controls. The fasting propionyl- (0.331 ± 0.019 vs 0.392 ± 0.017 μmol/L), butyryl- (0.219 ± 0.014 vs 0.265 ± 0.012), and isovalerylcarniUne (0.111 ± 0.008 vs 0.134 ± 0.008) levels were decreased in the UC patients. By contrast, the level of octanoyl- (0.147 ± 0.009 vs 0.114 ± 0.008), decanoyl- (0.180 ± 0.012 vs 0.137 ± 0.008), myristoyl- (0.048 ± 0.003 vs 0.039 ± 0.003), palmitoyl- (0.128 ± 0.006 vs 0.109 ± 0.004), palmitoleyl- (0.042±0.003 vs 0.031 ± 0.002) and oleylcarnitine (0.183 ± 0.007 vs 0.163 ± 0.007; P 〈 0.05 in all comparisons) were increased in the patients with UC. CONCLUSION: Our data suggest selective involvement of the carnitine esters in UC patients, probably due to their altered metabolism.展开更多
Supported Ir catalysts were prepared using layered double hydrotalcite‐like materials,such as Mg3Al1-xFex,containing Fe and Al species in varying amounts as supports.These Ir catalysts were applied for the selective ...Supported Ir catalysts were prepared using layered double hydrotalcite‐like materials,such as Mg3Al1-xFex,containing Fe and Al species in varying amounts as supports.These Ir catalysts were applied for the selective hydrogenation of cinnamaldehyde(CAL).When x was changed from 0(Ir/Mg3Al)to 1(Ir/Mg3Fe),the rate of CAL hydrogenation reached a maximum at approximately x=0.25,while the selectivity to unsaturated alcohol,i.e.,cinnamyl alcohol,monotonously increased from 44.9%to 80.3%.Meanwhile,the size of the supported Ir particles did not change significantly with x,remaining at 1.7-0.2 nm,as determined by transmission electron microscopy.The chemical state of Ir and Fe species in the Ir/Mg3Al1-xFex catalysts was examined by temperature programmed reduction by H2 and X‐ray photoelectron spectroscopy.The surface of the supported Ir particles was also examined through the in‐situ diffuse reflectance infrared Fourier‐transform of a probe molecule of CO.On the basis of these characterization results,the effects of Fe doping to Mg3Al on the structural and catalytic properties of Ir particles in selective CAL hydrogenation were discussed.The significant factors are the electron transfer from Fe2+in the Mg3Al1–xFex support to the dispersed Ir particles and the surface geometry.展开更多
AIM: To analyze the prevalence of germline MLH1 and MSH2 gene mutations and evaluate the clinical characteristics of Hungarian hereditary non-polyposis colorectal cancer (HNPCC) families. METHODS: Thirty-six kindreds ...AIM: To analyze the prevalence of germline MLH1 and MSH2 gene mutations and evaluate the clinical characteristics of Hungarian hereditary non-polyposis colorectal cancer (HNPCC) families. METHODS: Thirty-six kindreds were tested for mutations using conformation sensitive gel electrophoreses, direct sequencing and also screening for genomic rearrangements applying multiplex ligation-dependent probe amplifi cation (MLPA). RESULTS: Eighteen germline mutations (50%) were identifi ed, 9 in MLH1 and 9 in MSH2. Sixteen of these sequence alterations were considered pathogenic, the remaining two were non-conservative missense alterations occurring at highly conserved functional motifs. The majority of the defi nite pathogenic mutations (81%, 13/16) were found in families fulfilling the stringent Amsterdam Ⅰ/Ⅱ criteria, including three rearrangements revealed by MLPA (two in MSH2 and one in MLH1). However, in three out of sixteen HNPCC-suspected families (19%), a disease-causing alteration could be revealed. Furthermore, nine mutations described here are novel, and none of the sequence changes were found in more than one family.CONCLUSION: Our study describes for the f irst time the prevalence and spectrum of germline mismatch repair gene mutations in Hungarian HNPCC and suspected-HNPCC families. The results presented here suggest that clinical selection criteria should be relaxed and detection of genomic rearrangements should be included in genetic screening in this population.展开更多
The effects of sex, housing temperature, feed ingredients and slaughter weight on carcass and meat quality were investigated. A total of three-way crossbred (LWD) 100 pigs were used in a completely randomized design...The effects of sex, housing temperature, feed ingredients and slaughter weight on carcass and meat quality were investigated. A total of three-way crossbred (LWD) 100 pigs were used in a completely randomized design to study the influence of rearing condition, in the growing finishing period, the initial weight, finial weight, live weight, total feeding days, daily weight gain and total feed consumptions affected carcass traits and meat qualities. In conclusion, the rearing condition will give a significant effect on pork and carcass quality so that they can be controlled by changing the raising situation.展开更多
The aim of this study was to determine differences in body composition and physical fitness in elderly men and women. Five hundred twenty six subjects were included in this study, 272 were men (52%) and 254 women (...The aim of this study was to determine differences in body composition and physical fitness in elderly men and women. Five hundred twenty six subjects were included in this study, 272 were men (52%) and 254 women (48%). To determine the trend of changes of anthropometric parameters and physical fitness in people aged over 60, the authors were divided subjects in 5 age groups: 60-64, 65-69, 70-74, 75-79 and over 80 years of age. Decrease in strength is observed with the aging process so that the respondents aged 60-64 years significantly differ in the strength of the lower extremities of elderly subjects 70-74 and 75-79 years of age. Also this difference is noticeable if they compare with men and women. In this study, they found that there was an increase in the amount of adipose tissue, reducing the level of muscle activity and decreased muscle strength and endurance of the aging process.展开更多
This study was carried out at the Genetic Engineering Lab, College of Agriculture, University of Basrah. DNA was extracted from samples containing meat of commercial products in the Basrah markets. The products subjec...This study was carried out at the Genetic Engineering Lab, College of Agriculture, University of Basrah. DNA was extracted from samples containing meat of commercial products in the Basrah markets. The products subjected to analysis using polymerase chain reaction (PCR) with species specific repeat (SSR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques based on the sequence of the mitochondrial DNA cytochrome b gene (mtDNA cyt b gene). Then digestion with the Alu l restriction enzyme to establish a differential diagnosis detects and discriminates between meat species and adulteration in the products. SSR primers were applied, has been detected amplification of the encoded gene product, generated 221 bp in some imported minced and canned meat samples. The results show that SSR analysis produced a pattern that allowed a direct identification of horse and donkey meats in some imported minced and canned meat samples (Hana, Monde and Bavaria). The amplified 359 bp gene of mtDNA cyt b gene from samples in different product was cut using Alu 1 restriction enzyme resulting in restriction fragment length polymorphism (RFLP). Alu 1 was used to distinguish between the animals meat that belong to the family or one species. The digestion of the PCR product showed differences between products. Where the fragment length generated were 74, 76 and 189 bp. It belonged to horse meat. The fraud was detected in Hana, Monde and Bavaria products available in Basrah markets showing the presence of horse meat in these products that labeled as beef meats 100%. This revealed mtDNA cyt b gene as highly conserved and consequently a good molecular marker for diagnostic studies. Thus, this technique can be applied to food authentication for the identification of different species of animals in food products.展开更多
This study was carried out to investigate the growth performance, carcass yield and meat quality of large-type Korean native ducks (KND). A total of 420 day-old ducklings were divided into four treatments (seven re...This study was carried out to investigate the growth performance, carcass yield and meat quality of large-type Korean native ducks (KND). A total of 420 day-old ducklings were divided into four treatments (seven replicates/treatment, 15 birds/replicate) by strains (A and B) and gender (male and female) in a 2 × 2 factorial arrangement under complete randomized design. The body weight (BW) of female KND was higher at week 2 and for male it was higher in week 8 (P 〈 0.05). BW gain, feed intake and feed conversion ratio of male KND were higher than those of female KND (P 〈 0.05) during week 0 to week 8. The carcass yield of both strains increased from 6 weeks to 8 weeks (P 〈 0.05). The breast meat ratio was the highest at week 8 whereas the leg meat ratio was the highest at 6 weeks of age in both strains (P 〈 0.01). Lightness (L*), yellowness (b*) and juiciness of meat were the highest at 6 weeks (P 〈 0.05). Moisture, ash and fat contents of meat were the highest at 7 weeks (P 〈 0.05) and protein content was the highest at 8 weeks (P 〈 0.01). These results may provide the basic data on growth performance and meat quality of large-type KND, which can be used for genetic improvements and rearing at commercial scale.展开更多
Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hy...Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hydric restriction times (2 h and 4 h per day, continuously) were used in our trial carried in good sanitary conditions. Thirty six rabbits were divided at weaning (28 days) into three groups: R2 with restricted access to drinking water from 35 days to 80 days age of 2 h per day (from 8 a.m. to 10 a.m.), R4: access to drinking water 4 h per day (from 8 a.m. to 12 a.m.) and T (control group) with unlimited access to drinking water. Six animals from each group were slaughtered at 80 days of age. Carcass measurements and meat quality were studied. Histological study for kidneys was carried out. Relative weight of gastrointestinal tract increases with the degree of restriction: 16.5, 18.4 and 18.9 for T, R4 and R2, respectively (P 〈 0.05). Perirenal fat was lower in restricted groups but differences were not statically significant. Scapular fat decreases of 2/3 or 1/3 respectively with limiting access to drinking water of 2 h or 4 h per day. Compared to control group (T), meat of restricted groups contains more water: 70.3%, 70% and 69.3% respectively for R4, R2 and T (P 〈 0.01). The same tendency was observed for proteins: 19.2%, 19.3% and 17.7% for groups R2, R4 and T. Fat decreases in meat of restricted animals: 8.4%, 8.2% and 8.1% respectively for T, R4 and R2 (P 〈 0.05). Water restriction induced decrease of carcass and meat adiposity. There were no differences between pH post mortem and pH ultimate (after 24 h) between the three groups. Hydric restriction does not effect on kidney histology.展开更多
Dear Editor,Pelvic floor(PF)dysfunctions comprise of a wide spectrum of interrelated clinical conditions and represent an important health problem.The PF supporting system can be regarded as a loaded structure with ...Dear Editor,Pelvic floor(PF)dysfunctions comprise of a wide spectrum of interrelated clinical conditions and represent an important health problem.The PF supporting system can be regarded as a loaded structure with a complex geometry that supports the increase of abdominal pressure and maintain normal position of pelvic organs(Figure 1A).展开更多
For attaining the optimized locomotory performance of swimming fishes,both the passive visco-elastic properties of the fish body and the mechanical behavior of the active muscles should coordinate with the fish body’...For attaining the optimized locomotory performance of swimming fishes,both the passive visco-elastic properties of the fish body and the mechanical behavior of the active muscles should coordinate with the fish body’s undulatory motion pattern.However,it is difficult to directly measure the visco-elastic constitutive relation and the muscular mechanical performance in vivo.In the present paper,a new approach based on the continuous beam model for steady swimming fish is proposed to predict the fish body’s visco-elastic properties and the related muscle mechanical behavior in vivo.Given the lateral travelling-wave-like movement as the input condition,the required muscle force and the energy consumption are functions of the fish body’s visco-elastic parameters,i.e.the Young’s modulus E and the viscosity coefficient in the Kelvin model.After investigating the variations of the propagating speed of the required muscle force with the fish body’s visco-elastic parameters,we analyze the impacts of the visco-elastic properties on the energy efficiencies,including the energy utilization ratios of each element of the kinematic chain in fish swimming and the overall efficiency.Under the constraints of reasonable wave speed of muscle activation and the physiological feasibility,the optimal design of the passive visco-elastic properties can be predicted aiming at maximizing the overall efficiency.The analysis is based on the small-amplitude steady swimming of the carangiform swimmer,with typical Reynolds number varying from 2.5×104to 2.5×105,and the present results show that the non-dimensional Young’s modulus is 112±34,and the non-dimensional viscosity coefficient is 13 approximately.In the present estimated ranges,the overall efficiency of the swimming fish is insensitive to the viscosity,and its magnitude is about 0.11±0.02,in the predicted range given by previous study.展开更多
基金Supported by the grant of Ministry of Health,No.ETT 325/2003 and 595/2003the grant of Hungarian Science Foundation,No.OTKA T 35026 and T 49589from the National grant No.NKFP-4/005/2002
文摘AIM: To determine the plasma carnitine ester profile in adult patients with ulcerative culitis (UC) and compared with healthy control subjects. METHOD: Using ESI triple quadrupole tandem mass spectrometry, the carnitine ester profile was measured in 44 patients with UC and 44 age- and sex-matched healthy controls. RESULTS: There was no significant difference in the fasting free carnitine level between the patients with UC and the healthy controls. The fasting propionyl- (0.331 ± 0.019 vs 0.392 ± 0.017 μmol/L), butyryl- (0.219 ± 0.014 vs 0.265 ± 0.012), and isovalerylcarniUne (0.111 ± 0.008 vs 0.134 ± 0.008) levels were decreased in the UC patients. By contrast, the level of octanoyl- (0.147 ± 0.009 vs 0.114 ± 0.008), decanoyl- (0.180 ± 0.012 vs 0.137 ± 0.008), myristoyl- (0.048 ± 0.003 vs 0.039 ± 0.003), palmitoyl- (0.128 ± 0.006 vs 0.109 ± 0.004), palmitoleyl- (0.042±0.003 vs 0.031 ± 0.002) and oleylcarnitine (0.183 ± 0.007 vs 0.163 ± 0.007; P 〈 0.05 in all comparisons) were increased in the patients with UC. CONCLUSION: Our data suggest selective involvement of the carnitine esters in UC patients, probably due to their altered metabolism.
文摘Supported Ir catalysts were prepared using layered double hydrotalcite‐like materials,such as Mg3Al1-xFex,containing Fe and Al species in varying amounts as supports.These Ir catalysts were applied for the selective hydrogenation of cinnamaldehyde(CAL).When x was changed from 0(Ir/Mg3Al)to 1(Ir/Mg3Fe),the rate of CAL hydrogenation reached a maximum at approximately x=0.25,while the selectivity to unsaturated alcohol,i.e.,cinnamyl alcohol,monotonously increased from 44.9%to 80.3%.Meanwhile,the size of the supported Ir particles did not change significantly with x,remaining at 1.7-0.2 nm,as determined by transmission electron microscopy.The chemical state of Ir and Fe species in the Ir/Mg3Al1-xFex catalysts was examined by temperature programmed reduction by H2 and X‐ray photoelectron spectroscopy.The surface of the supported Ir particles was also examined through the in‐situ diffuse reflectance infrared Fourier‐transform of a probe molecule of CO.On the basis of these characterization results,the effects of Fe doping to Mg3Al on the structural and catalytic properties of Ir particles in selective CAL hydrogenation were discussed.The significant factors are the electron transfer from Fe2+in the Mg3Al1–xFex support to the dispersed Ir particles and the surface geometry.
基金Supported by the Hungarian Research Grants OTKA T-046570, NKFPI-00024/2005 and ETT 397/2006
文摘AIM: To analyze the prevalence of germline MLH1 and MSH2 gene mutations and evaluate the clinical characteristics of Hungarian hereditary non-polyposis colorectal cancer (HNPCC) families. METHODS: Thirty-six kindreds were tested for mutations using conformation sensitive gel electrophoreses, direct sequencing and also screening for genomic rearrangements applying multiplex ligation-dependent probe amplifi cation (MLPA). RESULTS: Eighteen germline mutations (50%) were identifi ed, 9 in MLH1 and 9 in MSH2. Sixteen of these sequence alterations were considered pathogenic, the remaining two were non-conservative missense alterations occurring at highly conserved functional motifs. The majority of the defi nite pathogenic mutations (81%, 13/16) were found in families fulfilling the stringent Amsterdam Ⅰ/Ⅱ criteria, including three rearrangements revealed by MLPA (two in MSH2 and one in MLH1). However, in three out of sixteen HNPCC-suspected families (19%), a disease-causing alteration could be revealed. Furthermore, nine mutations described here are novel, and none of the sequence changes were found in more than one family.CONCLUSION: Our study describes for the f irst time the prevalence and spectrum of germline mismatch repair gene mutations in Hungarian HNPCC and suspected-HNPCC families. The results presented here suggest that clinical selection criteria should be relaxed and detection of genomic rearrangements should be included in genetic screening in this population.
文摘The effects of sex, housing temperature, feed ingredients and slaughter weight on carcass and meat quality were investigated. A total of three-way crossbred (LWD) 100 pigs were used in a completely randomized design to study the influence of rearing condition, in the growing finishing period, the initial weight, finial weight, live weight, total feeding days, daily weight gain and total feed consumptions affected carcass traits and meat qualities. In conclusion, the rearing condition will give a significant effect on pork and carcass quality so that they can be controlled by changing the raising situation.
文摘The aim of this study was to determine differences in body composition and physical fitness in elderly men and women. Five hundred twenty six subjects were included in this study, 272 were men (52%) and 254 women (48%). To determine the trend of changes of anthropometric parameters and physical fitness in people aged over 60, the authors were divided subjects in 5 age groups: 60-64, 65-69, 70-74, 75-79 and over 80 years of age. Decrease in strength is observed with the aging process so that the respondents aged 60-64 years significantly differ in the strength of the lower extremities of elderly subjects 70-74 and 75-79 years of age. Also this difference is noticeable if they compare with men and women. In this study, they found that there was an increase in the amount of adipose tissue, reducing the level of muscle activity and decreased muscle strength and endurance of the aging process.
文摘This study was carried out at the Genetic Engineering Lab, College of Agriculture, University of Basrah. DNA was extracted from samples containing meat of commercial products in the Basrah markets. The products subjected to analysis using polymerase chain reaction (PCR) with species specific repeat (SSR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques based on the sequence of the mitochondrial DNA cytochrome b gene (mtDNA cyt b gene). Then digestion with the Alu l restriction enzyme to establish a differential diagnosis detects and discriminates between meat species and adulteration in the products. SSR primers were applied, has been detected amplification of the encoded gene product, generated 221 bp in some imported minced and canned meat samples. The results show that SSR analysis produced a pattern that allowed a direct identification of horse and donkey meats in some imported minced and canned meat samples (Hana, Monde and Bavaria). The amplified 359 bp gene of mtDNA cyt b gene from samples in different product was cut using Alu 1 restriction enzyme resulting in restriction fragment length polymorphism (RFLP). Alu 1 was used to distinguish between the animals meat that belong to the family or one species. The digestion of the PCR product showed differences between products. Where the fragment length generated were 74, 76 and 189 bp. It belonged to horse meat. The fraud was detected in Hana, Monde and Bavaria products available in Basrah markets showing the presence of horse meat in these products that labeled as beef meats 100%. This revealed mtDNA cyt b gene as highly conserved and consequently a good molecular marker for diagnostic studies. Thus, this technique can be applied to food authentication for the identification of different species of animals in food products.
文摘This study was carried out to investigate the growth performance, carcass yield and meat quality of large-type Korean native ducks (KND). A total of 420 day-old ducklings were divided into four treatments (seven replicates/treatment, 15 birds/replicate) by strains (A and B) and gender (male and female) in a 2 × 2 factorial arrangement under complete randomized design. The body weight (BW) of female KND was higher at week 2 and for male it was higher in week 8 (P 〈 0.05). BW gain, feed intake and feed conversion ratio of male KND were higher than those of female KND (P 〈 0.05) during week 0 to week 8. The carcass yield of both strains increased from 6 weeks to 8 weeks (P 〈 0.05). The breast meat ratio was the highest at week 8 whereas the leg meat ratio was the highest at 6 weeks of age in both strains (P 〈 0.01). Lightness (L*), yellowness (b*) and juiciness of meat were the highest at 6 weeks (P 〈 0.05). Moisture, ash and fat contents of meat were the highest at 7 weeks (P 〈 0.05) and protein content was the highest at 8 weeks (P 〈 0.01). These results may provide the basic data on growth performance and meat quality of large-type KND, which can be used for genetic improvements and rearing at commercial scale.
文摘Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hydric restriction times (2 h and 4 h per day, continuously) were used in our trial carried in good sanitary conditions. Thirty six rabbits were divided at weaning (28 days) into three groups: R2 with restricted access to drinking water from 35 days to 80 days age of 2 h per day (from 8 a.m. to 10 a.m.), R4: access to drinking water 4 h per day (from 8 a.m. to 12 a.m.) and T (control group) with unlimited access to drinking water. Six animals from each group were slaughtered at 80 days of age. Carcass measurements and meat quality were studied. Histological study for kidneys was carried out. Relative weight of gastrointestinal tract increases with the degree of restriction: 16.5, 18.4 and 18.9 for T, R4 and R2, respectively (P 〈 0.05). Perirenal fat was lower in restricted groups but differences were not statically significant. Scapular fat decreases of 2/3 or 1/3 respectively with limiting access to drinking water of 2 h or 4 h per day. Compared to control group (T), meat of restricted groups contains more water: 70.3%, 70% and 69.3% respectively for R4, R2 and T (P 〈 0.01). The same tendency was observed for proteins: 19.2%, 19.3% and 17.7% for groups R2, R4 and T. Fat decreases in meat of restricted animals: 8.4%, 8.2% and 8.1% respectively for T, R4 and R2 (P 〈 0.05). Water restriction induced decrease of carcass and meat adiposity. There were no differences between pH post mortem and pH ultimate (after 24 h) between the three groups. Hydric restriction does not effect on kidney histology.
基金supported by the National Natural Science Foundation of China(11272168)
文摘Dear Editor,Pelvic floor(PF)dysfunctions comprise of a wide spectrum of interrelated clinical conditions and represent an important health problem.The PF supporting system can be regarded as a loaded structure with a complex geometry that supports the increase of abdominal pressure and maintain normal position of pelvic organs(Figure 1A).
基金supported by the National Natural Science Foundation of China(Grants No.10832010)the Innovation Project of the Chinese Academy of Sciences(Grant No.KJCX2-YW-L05)
文摘For attaining the optimized locomotory performance of swimming fishes,both the passive visco-elastic properties of the fish body and the mechanical behavior of the active muscles should coordinate with the fish body’s undulatory motion pattern.However,it is difficult to directly measure the visco-elastic constitutive relation and the muscular mechanical performance in vivo.In the present paper,a new approach based on the continuous beam model for steady swimming fish is proposed to predict the fish body’s visco-elastic properties and the related muscle mechanical behavior in vivo.Given the lateral travelling-wave-like movement as the input condition,the required muscle force and the energy consumption are functions of the fish body’s visco-elastic parameters,i.e.the Young’s modulus E and the viscosity coefficient in the Kelvin model.After investigating the variations of the propagating speed of the required muscle force with the fish body’s visco-elastic parameters,we analyze the impacts of the visco-elastic properties on the energy efficiencies,including the energy utilization ratios of each element of the kinematic chain in fish swimming and the overall efficiency.Under the constraints of reasonable wave speed of muscle activation and the physiological feasibility,the optimal design of the passive visco-elastic properties can be predicted aiming at maximizing the overall efficiency.The analysis is based on the small-amplitude steady swimming of the carangiform swimmer,with typical Reynolds number varying from 2.5×104to 2.5×105,and the present results show that the non-dimensional Young’s modulus is 112±34,and the non-dimensional viscosity coefficient is 13 approximately.In the present estimated ranges,the overall efficiency of the swimming fish is insensitive to the viscosity,and its magnitude is about 0.11±0.02,in the predicted range given by previous study.