脉络宁注射液在不同输液中的稳定性考察

目的:研究脉络宁注射液在不同输液中的稳定性,明确临床使用范围。方法:用高效液相色谱法测定肉桂酸的含量,检测输液的pH值和澄明度。结果:脉络宁注射液在不同输液和不同条件下存放,其肉桂酸含量和pH值均在合格范围内,输液的澄明度符合要求。结论:脉络宁注射液与5%葡萄糖注射液、10%葡萄糖注射液、0.9%氯化钠注射液和葡萄糖氯化钠注射液相配伍,在45℃以下,保存6小时质量稳定。

HPLC Determination of Harpagoside and Cinnamic Acid in Radix Scrophulariae

A gradient HPLC method was established for the determination of harpagoside and cinnamic acid in Radix Scrophulariae (Xuanshen) and a proposition was put forward for the lowest content of the characteristic and active constituent (harpagoside) for qualified Radix Scrophulariae. The experimental conditions were as follows: Ultrasphere ODS column (250 mm 4.6 mm, 5 mm), mobile phase: acetonitrile-water (containing 1.0% acetic acid) (20:8050:50) (20 min), flow-rate 1 mLmin-1, room temperature, detection wavelength 278 nm. Twenty-eight samples of Xuan-shen (Radix Scrophulariae) from different districts of China were analyzed and the contents of harpagoside and cinnamic acid in Xuan-shen were 0.041~0.244% and 0.012~0.068% respectively. The recoveries (RSD)% were 97.13(0.80)% for harpagoside and 99.38(0.51)% for cinnamic acid. The method is simple and accurate. It can be used for the quality control of Radix Scrophulariae. We propose that the content of harpagoside in qualified Radix Scrophularia should be no less than 0.05%.

桂杏止咳颗粒提取工艺正交试验研究

目的通过正交试验,确定本品的最佳提取工艺。方法以肉桂酸为指标,采用正交试验对本品提取工艺进行优选。结果加药材8倍量的70%乙醇,提取3次,每次1.5小时。结论优选的工艺条件稳定、可行。