化瘀通络方对寻常性银屑病皮损及血浆内皮素的影响

目的探讨化瘀通络方治疗寻常性银屑病的临床疗效及其对血浆内皮素(endothelin,ET)的影响。方法将120例银屑病患者分为2组。治疗组60例用化瘀通络方治疗,对照组60例用消银颗粒治疗;另选30名健康者作为健康对照组。疗程8周后评价其临床疗效,并用放射免疫法检测治疗前后ET含量。结果 2组有效率分别为83.33%、56.67%,2组比较差异有统计学意义(u=3.38,P<0.005);银屑病患者血浆ET水平明显高于健康对照组,经治疗后2组ET均下降,治疗组下降更明显,与治疗前比较差异有统计学意义(P<0.05)。结论化瘀通络方治疗寻常性银屑病血瘀证有较好的临床疗效,且能降低ET水平。

针刺水沟、风府穴对脑缺血模型大鼠血一氧化氮及内皮素含量的影响

目的探讨针刺水沟-风府穴组治疗缺血性脑卒中的作用机制。方法将40只大鼠分为正常组、模型组、水沟-风府组(针刺大鼠水沟、风府穴)、非经非穴组(针刺大鼠臀部两处非经非穴点),每组10只。采用阻闭大鼠大脑中动脉制备永久性脑缺血模型。72h后观察各组大鼠神经功能评分,血清一氧化氮及血浆内皮素含量的变化。结果与模型组比较,水沟-风府组脑缺血大鼠神经功能评分明显降低(P<0.05),而非经非穴组无明显变化(P>0.05)。与模型组比较,非经非穴组大鼠血清一氧化氮及血浆内皮素含量无明显变化(P>0.05),水沟-风府组大鼠血清一氧化氮及血浆内皮素含量明显降低(P<0.05)。结论针刺水沟-风府穴组对缺血性脑卒中具有明显的改善作用,其机制可能与降低血液中一氧化氮、内皮素的含量有关。

内皮素鞘内注射后的脊髓形态学观察

对18只大鼠进行内皮素脊髓鞘内注射,观察及记录大鼠后肢功能状态,并将18只大鼠分为3个不同的时间处死,进行病理形态学观察。结果表明:3组均表现缺血缺氧病理改变。2及4h实验组病变重于30min实验组。脊髓下胸段、腰段病变重于颈段及骶尾段。同时发现,大鼠后肢的功能状态与其脊髓相应的病理形态学变化不呈正比。

氟伐他汀对颈动脉斑块及hs-CRP和ET-1的影响

目的:探讨氟伐他汀对颈动脉粥样斑块的消退作用及在斑块稳定性和炎症反应中的作用。方法:应用彩色多普勒超声检测出颈动脉粥样斑块形成者86例,随机分为观察组46例和对照组40例,观察组予以氟伐他汀40 mg/天,对照组予以饮食控制,均治疗一年,检测治疗前、后颈动脉粥样斑块的等级积分和Crouse积分,斑块数目,性质变化及血脂,血清高敏C-反应蛋白(hs-CRP)、内皮素-1(ET-1)水平。结果:观察组颈动脉粥样斑块的等级积分和Crouse积分,斑块数目,血脂,hs-CRP、ET-1水平均低于对照组,差异有统计学意义。结论:氟伐他汀可抑制炎症反应,改善内皮功能,稳定粥样斑块,并可有效消退颈动脉粥样斑块。

Effect of acetyl L-carnitine on human retinal pigment epithelium-19 cells in hypoxic conditions

AIM:To investigate the effect of acetyl-L-carnitine(ALCAR)on cell viability,morphological integrity,and vascular endothelial growth factor(VEGF)expression in human retinal pigment epithelium(ARPE-19)cells using a hypoxic model.METHODS:In the first set of experiments,the optimal CoCl_(2) dose was determined by exposing ARPE-19 cell cultures to different concentrations.To evaluate the effect of ALCAR on cell viability,five groups of ARPE-19 cell culture were established that included a control group,a sham group(200μM CoCl_(2)),and groups that received 1,10 and 100 mM doses of ALCAR combined with 200μM CoCl_(2),respectively.The cell viability was measured by MTT assay.The morphological characteristics of cells were observed by an inverted phase contrast microscope.The levels of VEGF and HIF-1α secretion by ARPE-19 cells were detected by enzyme linked immunosorbent assay(ELISA)assay.RESULTS:ARPE-19 cells were exposed to different doses of CoCl_(2) in order to create a hypoxia model.Nevertheless,when exposed to a concentration of 200μM CoCl_(2),a notable decrease in viability to 83% was noted.ALCAR was found to increase the cell viability at 1 mM and 10 mM concentrations,while the highest concentration(100 mM)did not have an added effect.The cell viability was found to be significantly higher in the groups treated with a concentration of 1 mM and 10 mM ALCAR compared to the Sham group(P=0.041,P=0.019,respectively).The cell viability and morphology remained unaffected by the greatest dose of ALCAR(100 mM).The administration of 10 mM ALCAR demonstrated a statistically significant reduction in the levels of VEGF and HIF-1α compared with the Sham group(P=0.013,P=0.033,respectively).CONCLUSION:The findings from the current study indicate that ALCAR could represent a viable therapeutic option with the potential to open up novel treatment pathways for retinal diseases,particular relevance for age-related macular degeneration(AMD).However,to fully elucidate ALCAR’s application potential in retinal diseases,additional investigation is necessary to clearly define the exact mechanisms involved.

Changes of bone mineral in steroid-induced osteonecrosis in rabbits

To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods： Seventy adult female Japanese White rabbits were randomly divided into five groups Animals in steroid group received an intramuscular injection of methylprednisolone acetate （4 mg/kg） once a week, and killed in four different time after first treatment. Animals in control group received an intramuscular injection with the same volume of normal saline, while 150 kU penicillin was injected twice a week for preventing infection in all groups. The contents of serum calcium （Ca） and phosphorus （P） were determined and the bone mineral densities of the femoral head and neck were measured at the 2nd, 4th, 8th and 10th weeks after first treatment. Meanwhile the general condition such as weight, spirit and hair of rabbits was observed. Results： Compared with the control group, the weight of rabbits in steroid group lost since the first week, and progressively aggravated; the bone mineral density of the femoral head and neck was lowered since the 2nd and 4th week respectively （P〈0.05, P〈0.01）, and lowered more quickly in femoral neck than in femoral head. In steroid group, the bone mineral density of the femoral neck at the 10th week was significantly lower than that at the 8th week （P〈0.05） Compared with the control group, the serum levels of Ca and P in steroid group were lowered since the 2nd week （P〈0.05, P〈0.01）. In steroid group, the serum Ca level at the 10th week was lower than that at the 8th week, and the serum P level waslowered more quickly than Ca level was. Conclusion： Osteonecrosis appears in rabbits shortly after corticoids are first administered, and osteonecrosis in rabbits is chronologically associated with the onset of hypocalcaemia, hypophosphatemia and lowered bone mineral density. Steroid-induced osteonecrosis may be relevant to bone mineral loss and density lowering of the femoral head and neck.

血瘀证目征与血管内皮细胞损伤的关系

目的:探讨血瘀证目征与血管内皮细胞损伤的关系。方法:将54只新西兰大白兔按随机分组法分为6组,分别为气虚血瘀组、气滞血瘀组、寒凝血瘀组、热毒血瘀组、外伤血瘀组、正常对照组。比较各组血瘀证目征积分,同时观测各组血清中vWF、ET-1水平的变化。结果:造模前各组血瘀证目征积分无显著差异:造模后各治疗组与对照组相比均有极显著差异(P<0.01):经补阳还王汤等中药2周治疗后,各治疗组与各自造模后相比均有极显著差异(P<0.01)。造模前各组血清ET-1,vWF的水平无显著差异,造模后血清ET-1,vWF水平各治疗组与对照组相比均有极显著差异(P<0.01),治疗后,各治疗组与各自造模后相比均有极显著差异(P<0.01)。结论:血瘀证目征是诊断血瘀证的有效方法,血瘀证目征与血管内皮细胞损伤有密切相关。