目的探讨骨质疏松性椎体压缩骨折(osteoporotic vertebral compression fracture,OVCF)患者腰骶区椎旁肌密度与椎体骨密度(vertebral bone mineral density,vBMD)之间的关系。方法回顾性分析了OVCF患者的前瞻性机构数据库(NCT05848167)...目的探讨骨质疏松性椎体压缩骨折(osteoporotic vertebral compression fracture,OVCF)患者腰骶区椎旁肌密度与椎体骨密度(vertebral bone mineral density,vBMD)之间的关系。方法回顾性分析了OVCF患者的前瞻性机构数据库(NCT05848167)。纳入2021年1月至2022年12月共167例患者资料,其中101例定量CT(quantitative computed tomography,QCT)检查资料可用的OVCF患者,女80例,男21例;女性中位年龄73(68.00,82.50)岁,男性中位年龄71(67.00,80.00)岁。在L_(3~5)椎体上终板水平进行椎旁肌密度和面积测量,并使用人工分割和定制编写的程序进行计算。利用QCT Pro软件在L_(1~2)水平进行vBMD测量和计算。按性别、年龄对所有分析进行分层分析。结果本研究发现男性患者的椎旁肌密度与vBMD有统计学相关。在调整年龄和性别后,L_(1~2)vBMD与L_(3)左侧多裂肌、L_(4)左侧竖脊肌、L_(4)右侧多裂肌、L_(5)左右双侧多裂肌密度之间存在统计学意义上的正相关,而在女性患者中未发现显著相关性。结论男性多裂肌密度和vBMD在不同的腰椎水平存在显著的正相关,这与年龄无关。在解释腰椎椎体和椎旁肌关联时,应考虑性别及区域性差异。展开更多
Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by cultured human arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected, and na...Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by cultured human arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected, and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electrophoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultraviolet spectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance (TBARS) with fluorescence spectrophotometry at 550 nm emission wavelength with excitation at 515 nm. Results: In comparison with N-HDL, the electrophoretic mobility of SMCs-cocultured HDL was increased, and the contents of CD, LOOH and TBARS HDL were very significantly higher than those of the control HDL (P<0.01). Conclusion: Oxidative modification of HDL can be induced by human arterial SMCs.展开更多
Objective： To investigate vascular endothelial growth factor （VEGF） expression and microvessel density （MVD） on the fibromyoma uteri treated with mifepristone. Methods： VEGF expression and MVD were counted on 60...Objective： To investigate vascular endothelial growth factor （VEGF） expression and microvessel density （MVD） on the fibromyoma uteri treated with mifepristone. Methods： VEGF expression and MVD were counted on 60 cases of the fibromyoma uteri by SP method, including 40 cases of mifepristone treated and 20 cases of untreated patients as controlled group. Results： VEGF positive expression rate and MVD in treated group were 3715% and 9.90 ＋ 5.95, which were lower than those in controlled group （80% and 16.36 ＋ 2.07; P 〈 0.05）. Meanwhile, in treated group, those in marked shrink in tumor size sub-group were lower than in not obvious sub-group （12.0% and 7.89 ＋ 4.36 vs. 80% and 11,29 ＋ 3.10; P 〈 0.05）. Conclusion： VEGF and MVD expression decreases in the fibromyoma uteri after treatment with mifepristone, suggesting that mifepristone could inhibit angiogenesis and blood supply resulting in tumor shrink.展开更多
To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods： Seventy adult female Japanese White rabbits were randomly divided into five grou...To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods： Seventy adult female Japanese White rabbits were randomly divided into five groups Animals in steroid group received an intramuscular injection of methylprednisolone acetate （4 mg/kg） once a week, and killed in four different time after first treatment. Animals in control group received an intramuscular injection with the same volume of normal saline, while 150 kU penicillin was injected twice a week for preventing infection in all groups. The contents of serum calcium （Ca） and phosphorus （P） were determined and the bone mineral densities of the femoral head and neck were measured at the 2nd, 4th, 8th and 10th weeks after first treatment. Meanwhile the general condition such as weight, spirit and hair of rabbits was observed. Results： Compared with the control group, the weight of rabbits in steroid group lost since the first week, and progressively aggravated; the bone mineral density of the femoral head and neck was lowered since the 2nd and 4th week respectively （P〈0.05, P〈0.01）, and lowered more quickly in femoral neck than in femoral head. In steroid group, the bone mineral density of the femoral neck at the 10th week was significantly lower than that at the 8th week （P〈0.05） Compared with the control group, the serum levels of Ca and P in steroid group were lowered since the 2nd week （P〈0.05, P〈0.01）. In steroid group, the serum Ca level at the 10th week was lower than that at the 8th week, and the serum P level waslowered more quickly than Ca level was. Conclusion： Osteonecrosis appears in rabbits shortly after corticoids are first administered, and osteonecrosis in rabbits is chronologically associated with the onset of hypocalcaemia, hypophosphatemia and lowered bone mineral density. Steroid-induced osteonecrosis may be relevant to bone mineral loss and density lowering of the femoral head and neck.展开更多
文摘目的探讨骨质疏松性椎体压缩骨折(osteoporotic vertebral compression fracture,OVCF)患者腰骶区椎旁肌密度与椎体骨密度(vertebral bone mineral density,vBMD)之间的关系。方法回顾性分析了OVCF患者的前瞻性机构数据库(NCT05848167)。纳入2021年1月至2022年12月共167例患者资料,其中101例定量CT(quantitative computed tomography,QCT)检查资料可用的OVCF患者,女80例,男21例;女性中位年龄73(68.00,82.50)岁,男性中位年龄71(67.00,80.00)岁。在L_(3~5)椎体上终板水平进行椎旁肌密度和面积测量,并使用人工分割和定制编写的程序进行计算。利用QCT Pro软件在L_(1~2)水平进行vBMD测量和计算。按性别、年龄对所有分析进行分层分析。结果本研究发现男性患者的椎旁肌密度与vBMD有统计学相关。在调整年龄和性别后,L_(1~2)vBMD与L_(3)左侧多裂肌、L_(4)左侧竖脊肌、L_(4)右侧多裂肌、L_(5)左右双侧多裂肌密度之间存在统计学意义上的正相关,而在女性患者中未发现显著相关性。结论男性多裂肌密度和vBMD在不同的腰椎水平存在显著的正相关,这与年龄无关。在解释腰椎椎体和椎旁肌关联时,应考虑性别及区域性差异。
文摘Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by cultured human arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected, and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electrophoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultraviolet spectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance (TBARS) with fluorescence spectrophotometry at 550 nm emission wavelength with excitation at 515 nm. Results: In comparison with N-HDL, the electrophoretic mobility of SMCs-cocultured HDL was increased, and the contents of CD, LOOH and TBARS HDL were very significantly higher than those of the control HDL (P<0.01). Conclusion: Oxidative modification of HDL can be induced by human arterial SMCs.
文摘Objective： To investigate vascular endothelial growth factor （VEGF） expression and microvessel density （MVD） on the fibromyoma uteri treated with mifepristone. Methods： VEGF expression and MVD were counted on 60 cases of the fibromyoma uteri by SP method, including 40 cases of mifepristone treated and 20 cases of untreated patients as controlled group. Results： VEGF positive expression rate and MVD in treated group were 3715% and 9.90 ＋ 5.95, which were lower than those in controlled group （80% and 16.36 ＋ 2.07; P 〈 0.05）. Meanwhile, in treated group, those in marked shrink in tumor size sub-group were lower than in not obvious sub-group （12.0% and 7.89 ＋ 4.36 vs. 80% and 11,29 ＋ 3.10; P 〈 0.05）. Conclusion： VEGF and MVD expression decreases in the fibromyoma uteri after treatment with mifepristone, suggesting that mifepristone could inhibit angiogenesis and blood supply resulting in tumor shrink.
文摘To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods： Seventy adult female Japanese White rabbits were randomly divided into five groups Animals in steroid group received an intramuscular injection of methylprednisolone acetate （4 mg/kg） once a week, and killed in four different time after first treatment. Animals in control group received an intramuscular injection with the same volume of normal saline, while 150 kU penicillin was injected twice a week for preventing infection in all groups. The contents of serum calcium （Ca） and phosphorus （P） were determined and the bone mineral densities of the femoral head and neck were measured at the 2nd, 4th, 8th and 10th weeks after first treatment. Meanwhile the general condition such as weight, spirit and hair of rabbits was observed. Results： Compared with the control group, the weight of rabbits in steroid group lost since the first week, and progressively aggravated; the bone mineral density of the femoral head and neck was lowered since the 2nd and 4th week respectively （P〈0.05, P〈0.01）, and lowered more quickly in femoral neck than in femoral head. In steroid group, the bone mineral density of the femoral neck at the 10th week was significantly lower than that at the 8th week （P〈0.05） Compared with the control group, the serum levels of Ca and P in steroid group were lowered since the 2nd week （P〈0.05, P〈0.01）. In steroid group, the serum Ca level at the 10th week was lower than that at the 8th week, and the serum P level waslowered more quickly than Ca level was. Conclusion： Osteonecrosis appears in rabbits shortly after corticoids are first administered, and osteonecrosis in rabbits is chronologically associated with the onset of hypocalcaemia, hypophosphatemia and lowered bone mineral density. Steroid-induced osteonecrosis may be relevant to bone mineral loss and density lowering of the femoral head and neck.
