The aim of the study was to investigate effect of transport times of up to 12 h on pigs＇ welfare. An observation box was located on the 3rd floor of the vehicle. Device to measure temperature, humidity and video came...The aim of the study was to investigate effect of transport times of up to 12 h on pigs＇ welfare. An observation box was located on the 3rd floor of the vehicle. Device to measure temperature, humidity and video camera to monitor pigs＇ behaviors were fitted in the box. Eighteen measurements were performed during two seasons for 4, 8, and 12 h of transport time with three replications. Meat samples were taken from longissimus dorsi （LD） and the carcases were chilled for 24 h at ＋4 ~C for pH determination. To determine cortisol, glucose, lactate, and creatine kinase concentration levels, blood samples were collected before and after transport from 90 pigs and from 20 controls that were not transported for control purposes. To evaluate behavioural alterations, frequency of events and durations were considered. Highest pH24 of 5.99 ＋ 0.29 occurred during summer at 12 h transport time. Cortisol concentrations elevated during short and decreased with an increase of transport time （P 〈 0.001）. Highest and lowest glucose concentrations for winter and summer were at 8 and 12 h transport time, respectively （P 〈 0.01）. Concentrations of lactate and creatine kinase positively correlated with transport time （P 〈 0.002）. Lying, sitting, rooting and vocalization behaviours correlated with transport time （P 〈 0.009）. Certain THI values during summer surpassed normal （〉 74） level during loading. The pH24 value correlated with transport time during summer. Lactate and creatine kinase concentrations increased with an increase in transport time but for Glucose, highest value was at 8 h transport time.展开更多
Objective To investigate the myocardial protective effects of pinacidil induced hyperpolarized arrest and compare with those afforded by conventional depolarized hyperkalemic arrestMethods Eighteen dogs were equal...Objective To investigate the myocardial protective effects of pinacidil induced hyperpolarized arrest and compare with those afforded by conventional depolarized hyperkalemic arrestMethods Eighteen dogs were equally divided into three groups: normothermic hyperpolarized group (Group A), hypothermic hyperpolarized group (Group B), and hyperkalemic group (Group C) Pinacidil (50μmol/L) containing 37℃ St Thomas solution (K+5mmol/L, 10ml/kg), pinacidil (50μmol/L, Sigma, USA) containing 4℃ St Thomas solution (K+ 5mmol/L, 10ml/kg) and 4℃ standard St Thomas solution (K+ 16mmol/L, 10ml/kg) were infused respectively through the aortic root after aorticclamping Heart arrest and its recovery, ultrastructure of the myocardium, the level of serum myocardial enzymes, and lipid peroxide and adenine cleotide of the myocardium were measuredHemodynamics during ischemia and after reperfusion were observedResults The percentages of normal mitochondria and glycogen did not change much during ischemia (except at 60 min) and after reperfusion in B Group, but declined markedly in Group C 30 min and 60 min after ischemia and 20 min after reperfusion (P<0.01) In Group A,they were lower than those of Group B before ischemia, but higher than those of Group C The recoveries of CO, SV, CI, LVSW, RVSW and MAP in Group B were significantly better than those in other two groups 15 min and 30 min after reperfusion (P<0.05and0.01, respectively) However, they were still better in Group A than those in Group C(P<0.05 and 0.01, respectively)The onset of heart arrest was faster in Groups C and B than that in Group A Highly elevated serum myocardial enzymes were observed 60 min after ischemia and 20 min after reperfusion in Group C, while they were only mild in the hyperpolarized groups, especially in Group B, and their recoveries were rapid Adenine nucleotides of the myocardium were better preserved in Group B than in other two groups 30 min, 60 min after ischemia, and 20 min after reperfusion (P<0.05 and 0.01, respectively)They were also much better in Group A than in GroupC(P<0.05and0.01,respectively)Lipid peroxide of the myocardium were significantly lower in Group B than in other groups 20 min after reperfusion (P<0.01),and they were lower in Group A than in Group C(P<0.05) Conclusions Myocardial protection for global ischemia during cardiopulmonary bypass (CPB) could be achieved with hyperpolarized heart arrest induced by pinacidil, an ATP sensitive potassium channel opener,especially in the hypothermic state The protection is weaker in normothermia but is still superior to that with traditional depolarized hyperkalemic arrest展开更多
A prominent example of seasonal phenotypic flexibility is the winter increase in thermogenic capacity （=summit metabolism, Msurn） in small birds, which is often accompanied by increases in pectoralis muscle mass and...A prominent example of seasonal phenotypic flexibility is the winter increase in thermogenic capacity （=summit metabolism, Msurn） in small birds, which is often accompanied by increases in pectoralis muscle mass and lipid catabolic capacity. Temperature or photoperiod may be drivers of the winter phenotype, but their relative impacts on muscle remodeling or lipid transport pathways are little known. We examined photoperiod and temperature effects on pectoralis muscle expres- sion of myostatin, a muscle growth inhibitor, and its tolloid-like protein activators （TLL-1 and TLL- 2）, and sarcolemmal and intracellular lipid transporters in dark-eyed juncos Junco hyemalis. We acclimated winter juncos to four temperature （3~C or 24~C） and photoperiod [short-day （SD） = 8L：16D; long-day （LD） = 16L：8D] treatments. We found that myostatin, TLL-I, TLL-2, and lipid transporter mRNA expression and myostatin protein expression did not differ among treatments, but treatments interacted to influence lipid transporter proteinexpression. Fatty acid translocase （FAT/CD36） levels were higher for cold SD than for other treatments. Membrane-bound fatty acid binding protein （FABPpm） levels, however, were higher for the cold LD treatment than for cold SD and warm LD treatments. Cytosolic fatty acid binding protein （FABPc） levels were higher on LD than on SD at 3℃, but higher on SD than on LD at 24℃. Cold temperature groups showed upregulation of these lipid transporters, which could contribute to elevated Msum compared to warm groups on the same photoperiod. However, interactions of temperature or photoperiod effects on muscle remodeling and lipid transport pathways suggest that these effects are context-dependent.展开更多
Objective To evaluate the clinical efficacy of tube moxibustion plus point-toward-point needling method for refractory facial paralysis.Methods A total of 100 patients with refractory facial paralysis who met the incl...Objective To evaluate the clinical efficacy of tube moxibustion plus point-toward-point needling method for refractory facial paralysis.Methods A total of 100 patients with refractory facial paralysis who met the inclusion criteria were randomized into an observation group and a control group,with 50 cases in each group.Both groups were treated with acupuncture by point-toward-point needling method,mainly in the affected eye,cheek and mouth areas.The observation group was given additional tube moxibustion after receiving the point-toward-point needling method,which inserted a tube moxibustion device into the external acoustic meatus 1 cm on the affected side for 20 min.Both groups were treated once a day,6 times a week,for 4 consecutive weeks.The House-Brackmann scale was scored before and after treatment,and the facial nerve electromyogram data were collected.Results The total effective rate of the observation group was 93.6%,which was higher than 64.6%of the control group,and the difference between the two groups was statistically significant(P<0.05).The proportion of new-onset facial paralysis complications(facial synkinesis,facial spasm,facial paralysis perversion,and crocodile tears)in the observation group was 6.4%,which was lower than 35.4%in the control group.The difference between the two groups was statistically significant(P<0.05).In the observation group,the numbers of cases after treatment with the ratio of action potential amplitude between the affected side and the healthy side increased by 10%–30%and over 30%were more than those in the control group.And the difference between the two groups was statistically significant(P<0.05).Conclusion Tube moxibustion plus point-toward-point needling method has a better effect in improving symptoms of refractory facial paralysis,decreasing the incidence of sequelae,and increasing clinical efficacy than the point-toward-point needling method alone.展开更多
To observe the clinical effect of acupuncture plus moving cupping with white mustard oil to treat lumbar myofascitis. Methods： A total of 90 patients with lumbar myofascitis who met the inclusion criteria were random...To observe the clinical effect of acupuncture plus moving cupping with white mustard oil to treat lumbar myofascitis. Methods： A total of 90 patients with lumbar myofascitis who met the inclusion criteria were randomly divided into an observation group and a control group, 45 cases in each group. The observation group received acupuncture and moving cupping with white mustard oil; the control group received acupuncture plus warm needling treatment. The treatment was given twice a week for a total of 4 weeks. The visual analog scale （VAS） and Chinese version of Roland-Morris disability questionnaire （RMDQ） score of pain before the treatment, and respectively after 1-week and 4-week treatment were recorded for efficacy evaluation. Results： After 4 weeks of treatment, the difference in total effective rate between the two groups was not significantly （P〉0.05）. After i week of treatment, there was no significant difference in VAS score between the two groups （P〉0.05）. After 4 weeks of treatment, the VAS score of the observation group was lower than that of the control group, and there was a significant difference between the two groups （P〈0.05）. After 4 weeks of treatment, the Chinese version of RMDQ scores of both groups were significantly decreased, there were statistical differences in comparing with those before treatment （both P〈0.05）. After treatment for 1 week and 4 weeks, the Chinese version of RMDQ score in the observation group was lower than that in the control group, with significant differences between the two groups （all P〈0.05）. Conclusion： Acupuncture plus moving cupping and acupuncture plus warm needling both can relieve the pain of patients with lumbar myofascitis, improve the function of back muscles, while the curative effect of acupuncture plus moving cupping is better.展开更多
Objective:To observe the clinical efficacy of warm needling moxibustion plus isokinetic muscle strength training for knee osteoarthritis(KOA).Methods:A total of 135 patients with KOA due to Yang deficiency and coagula...Objective:To observe the clinical efficacy of warm needling moxibustion plus isokinetic muscle strength training for knee osteoarthritis(KOA).Methods:A total of 135 patients with KOA due to Yang deficiency and coagulated cold were randomized into a warm needling moxibustion group,an isokinetic muscle strength training group,and a combined group by the random number table method,with 45 cases in each group.The warm needling moxibustion group was treated with warm needling moxibustion.The isokinetic muscle strength training group was treated with isokinetic muscle strength training.The combined group was treated with warm needling moxibustion plus isokinetic muscle strength training.The Western Ontario and McMaster Universities osteoarthritis index(WOMAC)and visual analog scale(VAS)were scored before and after treatment,and isokinetic indicators of peak torque(PT),total work(TW)and average power(AP)were evaluated.Results:The total effective rate of the combined group was 92.5%,which was significantly higher than 83.3%in the warm needling moxibustion group(P<0.05)and 72.5%in the isokinetic muscle strength training group(P<0.05).After treatment,the scores of WOMAC(total,pain,stiffness,and function)and VAS,and isokinetic indicators(PT,TW,and AP)were all improved compared with those before treatment(P<0.05)in all three groups.The differences among the three groups were statistically significant(P<0.05).The WOMAC total score and score of stiffness in the combined group were lower than those in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),and the scores in the warm needling moxibustion group were lower than those in the isokinetic muscle strength training group(P<0.05).The WOMAC score of pain and VAS score in the warm needling moxibustion group and the combined group were lower than those in the isokinetic muscle strength training group(P<0.05).The differences between the warm needling moxibustion group and the combined group were not statistically significant(P>0.05).The WOMAC function score in the combined group was lower than that in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),while there was no statistical difference between the warm needling moxibustion group and the isokinetic muscle strength training group(P>0.05).PT,TW,and AP in the combined group were higher than those in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),and they were higher in the isokinetic muscle strength training group than in the warm needling moxibustion group(P<0.05).Conclusion:Warm needling moxibustion plus isokinetic muscle strength training has a better effect in the treatment of KOA due to Yang deficiency and coagulated cold than either warm needling moxibustion or isokinetic muscle strength training alone.展开更多
文摘The aim of the study was to investigate effect of transport times of up to 12 h on pigs＇ welfare. An observation box was located on the 3rd floor of the vehicle. Device to measure temperature, humidity and video camera to monitor pigs＇ behaviors were fitted in the box. Eighteen measurements were performed during two seasons for 4, 8, and 12 h of transport time with three replications. Meat samples were taken from longissimus dorsi （LD） and the carcases were chilled for 24 h at ＋4 ~C for pH determination. To determine cortisol, glucose, lactate, and creatine kinase concentration levels, blood samples were collected before and after transport from 90 pigs and from 20 controls that were not transported for control purposes. To evaluate behavioural alterations, frequency of events and durations were considered. Highest pH24 of 5.99 ＋ 0.29 occurred during summer at 12 h transport time. Cortisol concentrations elevated during short and decreased with an increase of transport time （P 〈 0.001）. Highest and lowest glucose concentrations for winter and summer were at 8 and 12 h transport time, respectively （P 〈 0.01）. Concentrations of lactate and creatine kinase positively correlated with transport time （P 〈 0.002）. Lying, sitting, rooting and vocalization behaviours correlated with transport time （P 〈 0.009）. Certain THI values during summer surpassed normal （〉 74） level during loading. The pH24 value correlated with transport time during summer. Lactate and creatine kinase concentrations increased with an increase in transport time but for Glucose, highest value was at 8 h transport time.
基金theNationalNaturalScienceFoundationofChina (No .39760 0 71)
文摘Objective To investigate the myocardial protective effects of pinacidil induced hyperpolarized arrest and compare with those afforded by conventional depolarized hyperkalemic arrestMethods Eighteen dogs were equally divided into three groups: normothermic hyperpolarized group (Group A), hypothermic hyperpolarized group (Group B), and hyperkalemic group (Group C) Pinacidil (50μmol/L) containing 37℃ St Thomas solution (K+5mmol/L, 10ml/kg), pinacidil (50μmol/L, Sigma, USA) containing 4℃ St Thomas solution (K+ 5mmol/L, 10ml/kg) and 4℃ standard St Thomas solution (K+ 16mmol/L, 10ml/kg) were infused respectively through the aortic root after aorticclamping Heart arrest and its recovery, ultrastructure of the myocardium, the level of serum myocardial enzymes, and lipid peroxide and adenine cleotide of the myocardium were measuredHemodynamics during ischemia and after reperfusion were observedResults The percentages of normal mitochondria and glycogen did not change much during ischemia (except at 60 min) and after reperfusion in B Group, but declined markedly in Group C 30 min and 60 min after ischemia and 20 min after reperfusion (P<0.01) In Group A,they were lower than those of Group B before ischemia, but higher than those of Group C The recoveries of CO, SV, CI, LVSW, RVSW and MAP in Group B were significantly better than those in other two groups 15 min and 30 min after reperfusion (P<0.05and0.01, respectively) However, they were still better in Group A than those in Group C(P<0.05 and 0.01, respectively)The onset of heart arrest was faster in Groups C and B than that in Group A Highly elevated serum myocardial enzymes were observed 60 min after ischemia and 20 min after reperfusion in Group C, while they were only mild in the hyperpolarized groups, especially in Group B, and their recoveries were rapid Adenine nucleotides of the myocardium were better preserved in Group B than in other two groups 30 min, 60 min after ischemia, and 20 min after reperfusion (P<0.05 and 0.01, respectively)They were also much better in Group A than in GroupC(P<0.05and0.01,respectively)Lipid peroxide of the myocardium were significantly lower in Group B than in other groups 20 min after reperfusion (P<0.01),and they were lower in Group A than in Group C(P<0.05) Conclusions Myocardial protection for global ischemia during cardiopulmonary bypass (CPB) could be achieved with hyperpolarized heart arrest induced by pinacidil, an ATP sensitive potassium channel opener,especially in the hypothermic state The protection is weaker in normothermia but is still superior to that with traditional depolarized hyperkalemic arrest
文摘A prominent example of seasonal phenotypic flexibility is the winter increase in thermogenic capacity （=summit metabolism, Msurn） in small birds, which is often accompanied by increases in pectoralis muscle mass and lipid catabolic capacity. Temperature or photoperiod may be drivers of the winter phenotype, but their relative impacts on muscle remodeling or lipid transport pathways are little known. We examined photoperiod and temperature effects on pectoralis muscle expres- sion of myostatin, a muscle growth inhibitor, and its tolloid-like protein activators （TLL-1 and TLL- 2）, and sarcolemmal and intracellular lipid transporters in dark-eyed juncos Junco hyemalis. We acclimated winter juncos to four temperature （3~C or 24~C） and photoperiod [short-day （SD） = 8L：16D; long-day （LD） = 16L：8D] treatments. We found that myostatin, TLL-I, TLL-2, and lipid transporter mRNA expression and myostatin protein expression did not differ among treatments, but treatments interacted to influence lipid transporter proteinexpression. Fatty acid translocase （FAT/CD36） levels were higher for cold SD than for other treatments. Membrane-bound fatty acid binding protein （FABPpm） levels, however, were higher for the cold LD treatment than for cold SD and warm LD treatments. Cytosolic fatty acid binding protein （FABPc） levels were higher on LD than on SD at 3℃, but higher on SD than on LD at 24℃. Cold temperature groups showed upregulation of these lipid transporters, which could contribute to elevated Msum compared to warm groups on the same photoperiod. However, interactions of temperature or photoperiod effects on muscle remodeling and lipid transport pathways suggest that these effects are context-dependent.
文摘Objective To evaluate the clinical efficacy of tube moxibustion plus point-toward-point needling method for refractory facial paralysis.Methods A total of 100 patients with refractory facial paralysis who met the inclusion criteria were randomized into an observation group and a control group,with 50 cases in each group.Both groups were treated with acupuncture by point-toward-point needling method,mainly in the affected eye,cheek and mouth areas.The observation group was given additional tube moxibustion after receiving the point-toward-point needling method,which inserted a tube moxibustion device into the external acoustic meatus 1 cm on the affected side for 20 min.Both groups were treated once a day,6 times a week,for 4 consecutive weeks.The House-Brackmann scale was scored before and after treatment,and the facial nerve electromyogram data were collected.Results The total effective rate of the observation group was 93.6%,which was higher than 64.6%of the control group,and the difference between the two groups was statistically significant(P<0.05).The proportion of new-onset facial paralysis complications(facial synkinesis,facial spasm,facial paralysis perversion,and crocodile tears)in the observation group was 6.4%,which was lower than 35.4%in the control group.The difference between the two groups was statistically significant(P<0.05).In the observation group,the numbers of cases after treatment with the ratio of action potential amplitude between the affected side and the healthy side increased by 10%–30%and over 30%were more than those in the control group.And the difference between the two groups was statistically significant(P<0.05).Conclusion Tube moxibustion plus point-toward-point needling method has a better effect in improving symptoms of refractory facial paralysis,decreasing the incidence of sequelae,and increasing clinical efficacy than the point-toward-point needling method alone.
基金supported by Special Department Fund of Jiaxing Second Hospital of Zhejiang Province~~
文摘To observe the clinical effect of acupuncture plus moving cupping with white mustard oil to treat lumbar myofascitis. Methods： A total of 90 patients with lumbar myofascitis who met the inclusion criteria were randomly divided into an observation group and a control group, 45 cases in each group. The observation group received acupuncture and moving cupping with white mustard oil; the control group received acupuncture plus warm needling treatment. The treatment was given twice a week for a total of 4 weeks. The visual analog scale （VAS） and Chinese version of Roland-Morris disability questionnaire （RMDQ） score of pain before the treatment, and respectively after 1-week and 4-week treatment were recorded for efficacy evaluation. Results： After 4 weeks of treatment, the difference in total effective rate between the two groups was not significantly （P〉0.05）. After i week of treatment, there was no significant difference in VAS score between the two groups （P〉0.05）. After 4 weeks of treatment, the VAS score of the observation group was lower than that of the control group, and there was a significant difference between the two groups （P〈0.05）. After 4 weeks of treatment, the Chinese version of RMDQ scores of both groups were significantly decreased, there were statistical differences in comparing with those before treatment （both P〈0.05）. After treatment for 1 week and 4 weeks, the Chinese version of RMDQ score in the observation group was lower than that in the control group, with significant differences between the two groups （all P〈0.05）. Conclusion： Acupuncture plus moving cupping and acupuncture plus warm needling both can relieve the pain of patients with lumbar myofascitis, improve the function of back muscles, while the curative effect of acupuncture plus moving cupping is better.
文摘Objective:To observe the clinical efficacy of warm needling moxibustion plus isokinetic muscle strength training for knee osteoarthritis(KOA).Methods:A total of 135 patients with KOA due to Yang deficiency and coagulated cold were randomized into a warm needling moxibustion group,an isokinetic muscle strength training group,and a combined group by the random number table method,with 45 cases in each group.The warm needling moxibustion group was treated with warm needling moxibustion.The isokinetic muscle strength training group was treated with isokinetic muscle strength training.The combined group was treated with warm needling moxibustion plus isokinetic muscle strength training.The Western Ontario and McMaster Universities osteoarthritis index(WOMAC)and visual analog scale(VAS)were scored before and after treatment,and isokinetic indicators of peak torque(PT),total work(TW)and average power(AP)were evaluated.Results:The total effective rate of the combined group was 92.5%,which was significantly higher than 83.3%in the warm needling moxibustion group(P<0.05)and 72.5%in the isokinetic muscle strength training group(P<0.05).After treatment,the scores of WOMAC(total,pain,stiffness,and function)and VAS,and isokinetic indicators(PT,TW,and AP)were all improved compared with those before treatment(P<0.05)in all three groups.The differences among the three groups were statistically significant(P<0.05).The WOMAC total score and score of stiffness in the combined group were lower than those in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),and the scores in the warm needling moxibustion group were lower than those in the isokinetic muscle strength training group(P<0.05).The WOMAC score of pain and VAS score in the warm needling moxibustion group and the combined group were lower than those in the isokinetic muscle strength training group(P<0.05).The differences between the warm needling moxibustion group and the combined group were not statistically significant(P>0.05).The WOMAC function score in the combined group was lower than that in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),while there was no statistical difference between the warm needling moxibustion group and the isokinetic muscle strength training group(P>0.05).PT,TW,and AP in the combined group were higher than those in the warm needling moxibustion group and the isokinetic muscle strength training group(P<0.05),and they were higher in the isokinetic muscle strength training group than in the warm needling moxibustion group(P<0.05).Conclusion:Warm needling moxibustion plus isokinetic muscle strength training has a better effect in the treatment of KOA due to Yang deficiency and coagulated cold than either warm needling moxibustion or isokinetic muscle strength training alone.
