The variations of muscle tissus, peroxidase and Ca2+-ATPase activities and Vp (pasteurization value) were investigated with the fresh samples of grass carp (Ctenopharyngodon idellus) heated at different temperatur...The variations of muscle tissus, peroxidase and Ca2+-ATPase activities and Vp (pasteurization value) were investigated with the fresh samples of grass carp (Ctenopharyngodon idellus) heated at different temperatures, thus revealing the mechanism of denaturation of muscle protein by heating. The experimental results showed the great difference in muscle tissue structure before and after the samples heated. In the initial stage of heating the water loss accounted for 50% of the total. When the samples were separately heated at 75, 85, and 95℃, fh values (dehydration curve slope) were 5, 4.1 and 1.7, respectively, while Vp reaching 40 minutes were 22, 8 and 5 min, respectively. As to the time for deactivation of peroxidase in the heated samples at the various temperatures, it varied from 38, 10 and to less than 6 min, respectively and the deactivation rates of Ca2+-ATPase in 1 min were 50%, 88% and 100%, respectively. Consequently, the higher temperature the sample was heated, the faster rate of thermocon ductivity, the lower rate of water loss, and the faster rate of peroxidase deactivation it was. Also, the Vp for the least requirement of pasteurization (Vp≥40min) was obtained.展开更多
[ Objective] To detect the mRNA of muscle insulin-like growth factorl (IGF-I) in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growt...[ Objective] To detect the mRNA of muscle insulin-like growth factorl (IGF-I) in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quan- titative PCR, the muscle IGF-I mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [ Result] The IGF-I mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-I expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old ( P 〉0.05), but was lower than that of the latter with extremely significant difference ( P 〈0.01 ) from 30 to 60 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-I mRNA, but the expression level is different between these two species.展开更多
Environmental temperature is a major factor affecting animal performance in South China. With global warming, heat stress will become more and more serious. This paper reviewed the effects of heat stress on metabolism...Environmental temperature is a major factor affecting animal performance in South China. With global warming, heat stress will become more and more serious. This paper reviewed the effects of heat stress on metabolism of proteins, glucose, fat and energy in skeletal muscle and related mechanisms so as to provide theoretical guidance for alleviating heat stress and improving production performance of animal suffering from heat stress.展开更多
Liquid metals(LMs)are potential inorganic materials which could be applied in flexible and deformable electronics owing to their fluidity,low viscosity,high metallic conductivity,and low toxicity.However,recently repo...Liquid metals(LMs)are potential inorganic materials which could be applied in flexible and deformable electronics owing to their fluidity,low viscosity,high metallic conductivity,and low toxicity.However,recently reported sensing devices based on LMs required complex processes with high cost.Herein,a flexible three-dimensional(3 D)conductive network was prepared by coating LM droplets onto an electrospun thermoplastic polyurethane(TPU)fiber film.The LM is suspended between the TPU fibers and self-coalesces into a vertically buckled and laterally mesh-like structure,which provides good biocompatibility,conductivity,and stretchability simultaneously.The LM-TPU composite-filmbased flexible device demonstrates a multitude of desired features,such as a widely workable stretching range(0%-200%),sufficient sensitivity under stretching strain(gauge factor(GF)of 0.2 at 200%strain),and outstanding stability and durability(9000 cycles).In vitro biocompatibility experiments show that the LM-TPU composite film directly attached to the skin has excellent biocompatibility.Such strain sensorbased integrated monitoring systems could monitor human body motions in real time,such as muscle movement and joint motion,revealing application prospects in healthcare and human-machine interfacing.展开更多
文摘The variations of muscle tissus, peroxidase and Ca2+-ATPase activities and Vp (pasteurization value) were investigated with the fresh samples of grass carp (Ctenopharyngodon idellus) heated at different temperatures, thus revealing the mechanism of denaturation of muscle protein by heating. The experimental results showed the great difference in muscle tissue structure before and after the samples heated. In the initial stage of heating the water loss accounted for 50% of the total. When the samples were separately heated at 75, 85, and 95℃, fh values (dehydration curve slope) were 5, 4.1 and 1.7, respectively, while Vp reaching 40 minutes were 22, 8 and 5 min, respectively. As to the time for deactivation of peroxidase in the heated samples at the various temperatures, it varied from 38, 10 and to less than 6 min, respectively and the deactivation rates of Ca2+-ATPase in 1 min were 50%, 88% and 100%, respectively. Consequently, the higher temperature the sample was heated, the faster rate of thermocon ductivity, the lower rate of water loss, and the faster rate of peroxidase deactivation it was. Also, the Vp for the least requirement of pasteurization (Vp≥40min) was obtained.
文摘[ Objective] To detect the mRNA of muscle insulin-like growth factorl (IGF-I) in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quan- titative PCR, the muscle IGF-I mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [ Result] The IGF-I mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-I expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old ( P 〉0.05), but was lower than that of the latter with extremely significant difference ( P 〈0.01 ) from 30 to 60 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-I mRNA, but the expression level is different between these two species.
基金Supported by Key Project of Natural Science Foundation of Hubei Province(2013CFA100)National Natural Science Foundation of China(31472117)
文摘Environmental temperature is a major factor affecting animal performance in South China. With global warming, heat stress will become more and more serious. This paper reviewed the effects of heat stress on metabolism of proteins, glucose, fat and energy in skeletal muscle and related mechanisms so as to provide theoretical guidance for alleviating heat stress and improving production performance of animal suffering from heat stress.
基金supported by the National Natural Science Foundation of China(61625404,61888102,and 62174152)Foshan Innovative and Entrepreneurial Research Team Program(2018IT100031)。
文摘Liquid metals(LMs)are potential inorganic materials which could be applied in flexible and deformable electronics owing to their fluidity,low viscosity,high metallic conductivity,and low toxicity.However,recently reported sensing devices based on LMs required complex processes with high cost.Herein,a flexible three-dimensional(3 D)conductive network was prepared by coating LM droplets onto an electrospun thermoplastic polyurethane(TPU)fiber film.The LM is suspended between the TPU fibers and self-coalesces into a vertically buckled and laterally mesh-like structure,which provides good biocompatibility,conductivity,and stretchability simultaneously.The LM-TPU composite-filmbased flexible device demonstrates a multitude of desired features,such as a widely workable stretching range(0%-200%),sufficient sensitivity under stretching strain(gauge factor(GF)of 0.2 at 200%strain),and outstanding stability and durability(9000 cycles).In vitro biocompatibility experiments show that the LM-TPU composite film directly attached to the skin has excellent biocompatibility.Such strain sensorbased integrated monitoring systems could monitor human body motions in real time,such as muscle movement and joint motion,revealing application prospects in healthcare and human-machine interfacing.