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pur操纵子的启动子部分点突变对肌苷合成的影响
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作者 陈双喜 姚泉洪 +2 位作者 储炬 庄英萍 张嗣良 《中国医药工业杂志》 CAS CSCD 北大核心 2005年第8期464-467,共4页
以lacZ作为报告基因,对野生菌、肌苷低产菌和肌苷生产菌分别构建了研究型质粒pYH1206、pYH1618和pYH1620,并在大肠杆菌中表达,以研究pur操纵子与其阻遏蛋白PurR的相互作用对肌苷合成的影响。lacZ表达β-半乳糖苷酶相对活性的测定结果和... 以lacZ作为报告基因,对野生菌、肌苷低产菌和肌苷生产菌分别构建了研究型质粒pYH1206、pYH1618和pYH1620,并在大肠杆菌中表达,以研究pur操纵子与其阻遏蛋白PurR的相互作用对肌苷合成的影响。lacZ表达β-半乳糖苷酶相对活性的测定结果和阻遏蛋白PurR的序列分析表明,肌苷生产菌中pur操纵子的启动子部分-272位点处的突变是肌苷高产的主要原因。 展开更多
关键词 枯草芽孢杆菌 肌苷合成 pur操纵子 阻遏蛋白PurR 序列分析
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肌苷合成关键酶活性与肌苷积累之间的关系 被引量:6
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作者 宋勇波 蔡显鹏 +2 位作者 储炬 庄英萍 张嗣良 《微生物学报》 CAS CSCD 北大核心 2003年第3期361-365,共5页
测定比较了高产肌苷菌、低产肌苷菌和野生菌肌苷合成途径中PRPP转酰胺酶、sAMP合成酶、IMP脱氢酶和 5′ 核苷酸酶的比活以及活细胞的肌苷水解能力 ,进一步阐明了菌株产苷性能与肌苷合成途径关键酶活力间的密切关系。根据高产肌苷菌的酶... 测定比较了高产肌苷菌、低产肌苷菌和野生菌肌苷合成途径中PRPP转酰胺酶、sAMP合成酶、IMP脱氢酶和 5′ 核苷酸酶的比活以及活细胞的肌苷水解能力 ,进一步阐明了菌株产苷性能与肌苷合成途径关键酶活力间的密切关系。根据高产肌苷菌的酶学特性 ,确定了高产肌苷菌进一步基因工程改造的方向。研究表明酶学研究对有目的、有方向地进行高产菌株的选育工作具有重要意义。 展开更多
关键词 肌苷合成 活性 肌苷积累 PRPP转酰胺酶 sAMP合成 IMP脱氢酶 5′-核苷酸酶 水解能力
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略阳乌鸡肌肉组织中肌苷酸合成酶系相关基因表达规律的研究 被引量:5
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作者 路宏朝 张涛 王令 《中国家禽》 北大核心 2016年第3期5-9,共5页
选取胚龄为8、11、15、18、21 d、出雏后3、6、120 d的略阳乌鸡为研究对象,探讨肌肉中肌苷酸合成酶系相关基因的表达规律。采用实时荧光定量PCR检测不同阶段的肌肉组织中PAICS、PPAT、GART和ADSL四种基因的表达水平。结果表明,PPAT、GAR... 选取胚龄为8、11、15、18、21 d、出雏后3、6、120 d的略阳乌鸡为研究对象,探讨肌肉中肌苷酸合成酶系相关基因的表达规律。采用实时荧光定量PCR检测不同阶段的肌肉组织中PAICS、PPAT、GART和ADSL四种基因的表达水平。结果表明,PPAT、GART、PAICS、ADSL基因在胚胎期都大量表达,PAICS基因在胚胎早期(胚龄第8天)表达量最高,PPAT和GART在胚胎中期(胚龄第11天)表达量最高,ADSL基因的表达随着胚龄的增加呈上升趋势直到成年。另外,腿肌组织中PAICS、PPAT、GART、ADSL基因的表达水平在幼年期和成年期基本一致;但胸肌中四种基因表达量都明显高于腿肌,说明胸肌中肌苷酸的合成量可能会高于腿肌。 展开更多
关键词 略阳乌鸡 肌苷合成 表达规律
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Beneficial effects of fenugreek glycoside supplementation in male subjects during resistance training: A randomized controlled pilot study
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作者 Sachin Wankhede Vishwaraman Mohan Prasad Thakurdesai 《Journal of Sport and Health Science》 SCIE 2016年第2期176-182,共7页
Purpose: To evaluate the efficacy and safety of the glycoside fraction of fenugreek (Trigonellafoenum-graecum) seeds (Fenu-FG) on physiologi- cal parameters related to muscle anabolism, androgenic hormones, and b... Purpose: To evaluate the efficacy and safety of the glycoside fraction of fenugreek (Trigonellafoenum-graecum) seeds (Fenu-FG) on physiologi- cal parameters related to muscle anabolism, androgenic hormones, and body fat in healthy male subjects during an 8-week resistance training program using a prospective, randomized, double-blind, placebo controlled design. Methods: Sixty healthy male subjects were randomized to ingest capsules of Fenu-FG (1 capsule of 300 rag, twice per day) or the matching placebo at a 1:1 ratio. The subjects participated in a supervised 4-day per week resistance-training program for 8 weeks. The outcome measurements were recorded at recruitment (baseline) and at the end of the treatment (8 weeks). The efficacy outcome included serum testosterone (total and free) levels, muscle strength and repetitions to failure, metabolic markers for anabolic activity (serum creatinine and blood urea nitrogen), and % body fat. The standard safety measurements such as adverse events monitoring, vital signs, hematology, biochemistry, and urinalysis were performed. Results: Fenu-FG supplementation demonstrated significant anabolic and androgenic activity as compared with the placebo. Fenu-FG treated subjects showed significant improvements in body fat without a reduction in muscle strength or repetitions to failure. The Fenu-FG supplemen- tation was found to be safe and well-tolerated. Conclusion: Fenu-FG supplementation showed beneficial effects in male subjects during resistance training without any clinical side effects. 展开更多
关键词 ANABOLIC Androgenic Fenugreek seeds GLYCOSIDES Randomized controlled study Resistance training
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Baicalin attenuates high fat diet-induced insulin resistance and ectopic fat storage in skeletal muscle,through modulating the protein kinase B/Glycogen synthase kinase 3 beta pathway 被引量:20
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作者 XI You-Li LI Hong-Xia +6 位作者 CHEN Chen LIU Ya-Qun LV Hong-Mei DONG Shi-Qi LUO Er-Fei GU Ming-Bo LIU Hua 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2016年第1期48-55,共8页
Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed a... Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed at evaluating the therapeutic effects of baicalin on insulin resistance and skeletal muscle ectopic fat storage in high fat diet-induced mice,and exploring the potential molecular mechanisms.Insulin resistance in mice was induced with a high fat diet for 16 weeks.Animals were then treated with three different doses of baicalin(100,200,and 400 mg·kg^(-1)·d^(-1)for 14 weeks.Fasting blood glucose,fasting serum insulin,glucose tolerance test(GTT),insulin tolerance test(ITT),and skeletal muscle lipid deposition were measured.Additionally,the AMP-activated protein kinase/acetyl-CoA carboxylase and protein kinase B/Glycogen synthase kinase 3 beta pathways in skeletal muscle were further evaluated.Baicalin significantly reduced the levels of fasting blood glucose and fasting serum insulin and attenuated high fat diet induced glucose tolerance and insulin tolerance.Moreover,insulin resistance was significantly reversed.Pathological analysis revealed baicalin dose-dependently decreased the degree of the ectopic fat storage in skeletal muscle.The properties of baicalin were mediated,at least in part,by inhibition of the AMPK/ACC pathway,a key regulator of de novo lipogenesis and activation of the Akt/GSK-3β pathway,a key regulator of Glycogen synthesis.These data suggest that baicalin,at dose up to 400 mg·kg^(-1)·d^(-1),is safe and able to attenuate insulin resistance and skeletal muscle ectopic fat storage,through modulating the skeletal muscle AMPK/ACC pathway and Akt/GSK-3β pathway. 展开更多
关键词 BAICALIN Insulin resistance Skeletal muscle ectopic fat storage Protein kinase B Glycogen synthase kinase 3 beta
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