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老年大鼠血管平滑肌中多巴胺受体DA1亚型的改变
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作者 张红 张梅 +1 位作者 张轩萍 赵荣瑞 《山西医科大学学报》 CAS 1996年第S1期7-9,共3页
选用fenoldopam作为DA1受体的特异性激动剂,利用离体血管环的方法对老年(18月龄)大鼠与年轻大鼠(3月龄)血管平滑肌中多巴胺受体DA1亚型的差别进行了观察。结果显示,老年大鼠主动脉、肾动脉、肠系膜动脉由去甲... 选用fenoldopam作为DA1受体的特异性激动剂,利用离体血管环的方法对老年(18月龄)大鼠与年轻大鼠(3月龄)血管平滑肌中多巴胺受体DA1亚型的差别进行了观察。结果显示,老年大鼠主动脉、肾动脉、肠系膜动脉由去甲肾上腺素引起的最大收缩反应与年轻大鼠无明显差别,但3种血管由fenoldopam引起的累积浓度-舒张反应曲线均明显右移,提示老年大鼠血管平滑肌中DA1受体显著下调。 展开更多
关键词 受体.多巴胺 大鼠肌.平滑.血管
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肾血管平滑肌脂肪瘤的诊断与治疗(附26例报告)
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作者 潘正跃 葛根 +1 位作者 黄国华 李国荣 《南昌大学学报(医学版)》 CAS 1997年第4X期71-73,共3页
为提高肾血管平滑肌脂肪瘤的诊断与治疗水平,本文总结了26例肾血管平滑肌脂肪瘤(占同期肾肿瘤的20.3%)的临床资料。21例行手术治疗,其中单纯切除肿瘤7例,肾切除14例;未行手术5例。通过观察随访,得出:B超和CT是术前诊断此肿瘤... 为提高肾血管平滑肌脂肪瘤的诊断与治疗水平,本文总结了26例肾血管平滑肌脂肪瘤(占同期肾肿瘤的20.3%)的临床资料。21例行手术治疗,其中单纯切除肿瘤7例,肾切除14例;未行手术5例。通过观察随访,得出:B超和CT是术前诊断此肿瘤的最好手段,治疗仍以手术为主,对4cm以下的肿瘤应采取定期随访观察。 展开更多
关键词 肾肿瘤/诊断 肾肿瘤/外科手术 肌.平滑.血管 脂肪瘤 病例报告
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家兔肝动脉平滑肌中多巴胺受体亚型分析
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作者 张梅 张红 +1 位作者 张炜芳 赵荣瑞 《山西医科大学学报》 CAS 1996年第S1期4-6,共3页
选用FODA和PBDA分别作为DA1和DA2受体的特异性激动剂;以SCH2339。和domperidone分别作为DA1和DA2受体的阻断剂,利用离体血管环的方法对家兔肝动脉平滑肌中多巴胺受体的存在情况进行了观察。结... 选用FODA和PBDA分别作为DA1和DA2受体的特异性激动剂;以SCH2339。和domperidone分别作为DA1和DA2受体的阻断剂,利用离体血管环的方法对家兔肝动脉平滑肌中多巴胺受体的存在情况进行了观察。结果表明:多巴胺受体两亚型(DA1、DA2)均在肝动脉平滑肌中存在。 展开更多
关键词 受体.多巴胺 受体亚型 肝动脉肌.平滑.血管
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衰老大鼠主动脉及其平滑肌细胞G蛋白信使核糖核酸的变化
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作者 周元国 王正国 熊仁平 《中华老年医学杂志》 CAS CSCD 北大核心 1997年第2期110-113,共4页
目的衰老引起的血管功能减退与G蛋白有关,本实验研究24月龄大鼠主动脉及平滑肌细胞(SMC)中G蛋白α亚基的信使核糖核酸(mRNA)变化。方法用Gsα、Gi2α、Gi3α和Goα的cDNA探针按Northernblot... 目的衰老引起的血管功能减退与G蛋白有关,本实验研究24月龄大鼠主动脉及平滑肌细胞(SMC)中G蛋白α亚基的信使核糖核酸(mRNA)变化。方法用Gsα、Gi2α、Gi3α和Goα的cDNA探针按Northernblot和dotblot法对6和24月龄大鼠主动脉和主动脉SMC相应的G蛋白α亚基mR-NA进行定量分析。结果Goα在主动脉及其SMC中均为2条带(3.5和3.8kb),Gi3α和Gsα在二者中各为1条带(3.2和1.8kb),但Gi2α主动脉有2.1kb的强带和1.7kb的弱带,而SMC只有2.1kb的1条带。主动脉中各Gα均无显著变化,而SMC中Goα、GsαmRNA轻度升高,Gi2α显著升高(P<0.05)。结论主动脉SMC中G蛋白α亚基的mRNA表达是有变化的,其趋势与主动脉基本一致,但变化更明显一些。 展开更多
关键词 G蛋白质类 主动脉 肌.平滑.血管
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Urocortin, the neuropeptide, inhibits the viability of ECV304 cells and rat vascular smooth muscle cells
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作者 陈洁 汪红仪 +3 位作者 陶金 徐华娥 杨荣 李胜男 《Journal of Nanjing Medical University》 2004年第1期1-3,共3页
Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' t... Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 cells and VSMC by using a tetrazolium (MTT) assay.Results: Ucn (10 -7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively(P<0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC. Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling (VR). 展开更多
关键词 UROCORTIN ECV304 vascular smooth muscle cells MTT assay ATP-sensitive potassium channels
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Arsenic trioxide inhibites transgenic tumor necrosis factor-α promoter activity in vascular smooth muscle cells and THP-1 monocytes in vitro
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作者 张卓琦 曹希传 黄永麟 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第2期134-138,共5页
Aim This study was to evaluate the effect of arsenic trioxide (As2O3) on the transgenic TNF-α promoter activity in cultured vascular smooth muscle cells (VSMCs) and THP-1 monocytes. Methods Human TNF-α promoter ... Aim This study was to evaluate the effect of arsenic trioxide (As2O3) on the transgenic TNF-α promoter activity in cultured vascular smooth muscle cells (VSMCs) and THP-1 monocytes. Methods Human TNF-α promoter was constructed by reporter gene system and was transiently transfected into VSMCs and THP-1 in vitro. The promoter activity was tested by luciferase activity with or without LPS and Ang Ⅱ stimulation, before and after different dosage of As2O3 treatment. Results 1. TNF-α promoter effectively expressed in VSMCs and THP-1 compared with CMV promoter (58.3% and 80.9%, respectively). Both LPS and Ang Ⅱ significantly up-regulated TNF-α promoter activity (P〈0.05). 2. As2O3 significantly inhibited, both intact and LPS/Ang Ⅱ stimulated promoter activity, in a dose dependent manner (P〈0.05), and in both cell type. Conclusion These results manifested that, the inhibition effect of As2O3 on the activity of human TNF-α promoter indicated its potential inhibition on pro-inflammatory cytokine genes expression at transcriptional level and its potential anti-inflammatory property in the cardiovascular system. 展开更多
关键词 Arsenic trioxide TNF-α promoter INFLAMMATION Smooth muscle cells VASCULAR MONOCYTES
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Effect of Intravascular Irradiation on Intimal Smooth Muscle Cells Proliferation and Apoptosis in Balloon Injuried Arteries
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作者 马根山 王连生 +5 位作者 黄峻 冷静 彭韬 杨志健 尹航 曹克将 《Journal of Nanjing Medical University》 2003年第4期159-165,共7页
Objective: To investigate the effect of intravascular in radiation on thearterial wall smooth muscle cells (SMCs) proliferation and apoptosis after iliac artery bollominjury in figs. Methods: Twenty-seven miniature fi... Objective: To investigate the effect of intravascular in radiation on thearterial wall smooth muscle cells (SMCs) proliferation and apoptosis after iliac artery bollominjury in figs. Methods: Twenty-seven miniature figs were divided into three groups. All pigsunderwent iliac artery balloon over-stretch. An^(192) Ir source through afterloader was positionedat the injuried segments to give 10 Gy in 9 pigs and 20 Gy in the other 9 pigs, and the rest 9 pigswere, used as control group. The pigs were killed on the 3rd, 10th and 28th days respectively forobservation. The injured segments were processed to examine SMCs proliferation by proliferation cellnuclear antigen (PCNA) and apopto-sis by terminal deoxynucleotidyl transferase-mediated dUTPnick-end labeling (TUNEL). Results: PC-NA index analysis has some that SMCs proliferation inneointima was significantly inhibited in irradiation group on the 10th and 28th days. The value forintimal SMCs apoptosis in control vs 10 Gy and 20 Gy irradiation groups were: (1. 185+-0. 49)% vs(2. 27+-0. 49)%(P>0. 05) and (1. 85+-0. 49)% vs (2. 53+-0. 45)%(P<0. 05), at the 10th day; (1.61+-0. 35)% vs (3. 11+-0. 51)%(P<0. 05), and (1.61+-0. 35)% vs (7. 05+-1. 82)% (P<0. 05), on the28th day. In irradiated arteries, the maximal incidence of intimal SMCs apoptosis was (7. 05+--1.82)% in 20 Gy group vs (3. 11+-0. 51)% in 10 Gy group (P<0. 05), on the 28th day. In the same doseirradiation group, the incidence of intimal SMCs apoptosis was higher on the 28th day than that onthe 10th day. Conclusion: Intra-arterial gamma irradiation can inhibit intimal SMCs proliferationand stimulate SMCs apoptosis in balloon-in jured arteries. These may be contributive to preventionof restenosis of arteries after balloon injury. 展开更多
关键词 intravascular irradiation smooth muscle cells PROLIFERATION APOPTOSIS RESTENOSIS
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Effects of Tetrandrine on Cytosolic Free Calcium in Cultured Bovine Aortic Smooth Muscle Cells 被引量:1
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作者 李新天 王幼林 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第2期38-41,共4页
\ The effects of tetrandrine (Tet) on cytosolic free calcium ([Ca2+]i) in subcultured bovine aortic smooth muscle cells (SMC) were studied by Fura2 and ARCMMIC cation measurement system. Tet (1~100 μmol·L-1) ... \ The effects of tetrandrine (Tet) on cytosolic free calcium ([Ca2+]i) in subcultured bovine aortic smooth muscle cells (SMC) were studied by Fura2 and ARCMMIC cation measurement system. Tet (1~100 μmol·L-1) had no effect on the resting [Ca2+]i, but had inhibitory effects on [Ca2+]i elevation induced by high K+, 5HT, ATP, Ang II and NE in the presence of extracellular Ca2+. High concentration of Tet also inhibited Pheinduced [Ca2+]i elevation in absence of extracellular Ca2+. Tet (1~100 μmol·L-1) inhibited KCl (60 mmol·L-1) induced [Ca2+]i elevation in dosedependent manner, the IC50 value was 9.2 (95% confidence limits: 5.7~14.9) mmol·L-1. The results suggested that Tet had blocking effects on both VOC and ROC in bovine aortic SMC. It appears that the mechanisms of blocking effect of Tet on ROC might be primarily due to its Ca2+ entry blocking effects. 展开更多
关键词 TETRANDRINE Vascular smooth muscle cell Fura2 Calcium channel blockers
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Epithelioid angiomyolipoma of the liver:Cross-sectional imaging findings of 10 immunohistochemically-verified cases 被引量:26
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作者 Peng-Ju Xu Yan Shan +3 位作者 Fu-Hua Yan Yuan Ji Ying Ding Mei-Lin Zhou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第36期4576-4581,共6页
AIM: To retrospectively evaluate the computed tomography (CT)/magnetic resonance imaging (MRI) imaging features of epithelioid angiomyolipoma of the liver (Epi-HAML), with pathology as a reference. METHODS: Th... AIM: To retrospectively evaluate the computed tomography (CT)/magnetic resonance imaging (MRI) imaging features of epithelioid angiomyolipoma of the liver (Epi-HAML), with pathology as a reference. METHODS: The CT/MRI findings (number, diameter, lobar location, and appearance of lesions) in a series of 10 patients with 12 pathologically proven epithelioid angiomyolipomas of the liver were retrospectively analyzed. The imaging features, including attenuation/ signal intensity characteristics, presence of fat, hypervascular, outer rim, and vessels within lesion, were evaluated and compared with that of non-Epi- HAML in 11 patients (13 lesions). The Fisher exact test was used to compare difference in probability of imaging features between the two types. RESULTS: For 21 patients, CT images of 15 patients and MR images of six patients were available. No patient underwent two examinations. For the 15 patients with a CT scan, all HAML lesions in the two groups (10 Epi-HAML and seven non-Epi-HAML) manifested as hypoattenuation. For the six patients with MRI, all lesions (two Epi-HAML and six non-Epi- HAML) were hypointense on TlWI (fat suppression) and hyperintense on T2WI. There were 10 non-Epi-HAML, but only two Epi-HAML lesions showed the presence of fat, which significantly different between the two types (P = 0.005). On the dynamic contrast enhancement (DCE) imaging, eight Epi-HAML, and 13 non-Epi lesions manifested as hypervascular. Punctate or curved vessels were displayed in 10 Epi-HAML as well as in nine non- Epi lesions and outer rim enhancement could be found with eight Epi-HAML as well as six non-Epi lesions. CONCLUSION: Little or no presence of adipose tissue was found to be an imaging feature of Epi- HAML, compared with the non-Epi type. In addition, hypervascularity with opacification of central punctiform or filiform vessels on DCE would be a characteristic enhancement pattern for Epi-HAML. 展开更多
关键词 Epithelioid angiomyolipoma LIVER Immunohistochemical staining Magnetic resonance imaging Computed X-ray tomography
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Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture 被引量:1
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作者 Li-jie FAN Takeshi KARINO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第8期623-629,共7页
Background:The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially imp... Background:The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans. However, it is not well known how serum factors affect the adhesion of monocytes. Methods: We have studied the effect of fetal calf serum (FCS), which we considered a source of LDL, on the adhesion of monocytes to endothelial cells (ECs) by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells (EC monoculture) and a co-culture with bovine aortic smooth muscle cells (EC-SMC co-culture). Results: It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells, and the higher the concentration of FCS in the medium, the greater the adhesion of THP-1 cells to endothelial cells. Adhesion of THP-1 cells to an EC-SMC co-culture was approximately twofold greater than that to an EC monoculture, and after adhering to endothelial cells, many THP-1 cells trans-migrated into the layer of smooth muscle cells. Conclusion: The results suggest that the elevation of the LDL (cholesterol) level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytes to the endothelium and their subsequent migration into subendothelial spaces. 展开更多
关键词 Endothelial cells (ECs) Smooth muscle cells (SMCs) MONOCYTE THP-1 cells Low-density lipoprotein (LDL) Adhesion Transendothelial migration Serum concentration
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Yangxueqingnao particles inhibit rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid 被引量:6
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作者 蔡巍 许毅 +3 位作者 陈君柱 黄淑如 卢震亚 王战坤 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第9期892-896,共5页
Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) prolif- eration induced by lysophosphatidic acid (LPA). Methods: The amount of 3H-TdR (3H-thymidine) admixed in cu... Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) prolif- eration induced by lysophosphatidic acid (LPA). Methods: The amount of 3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation end product malondialdehyde (MDA) content of the VSMC were assayed. Results: 1×10?9, 1×10?8, 1×10?7 mol/L LPA in a concentration dependent manner, induced the amount of 3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P<0.01) respectively in the amount of 3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P<0.05), 29.6% (P<0.01), and 48.9% (P<0.01) respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P<0.01) respectively, in the 1×10?7 mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion. 展开更多
关键词 Yangxueqingnao particles Lysophosphatidic acid Vascular smooth muscle cell PROLIFERATION
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Altered serum level of cartilage oligomeric matrix protein and its association with coronary calcification in patients with coronary heart disease 被引量:16
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作者 Fang-Fang WANG Lahati HA +3 位作者 Hai-Yi YU Lin MI Jiang-Li HAN Wei GAO 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第2期87-92,共6页
Background Cartilage oligomeric matrix protein (COMP) is mainly found in the skeletal system and vascular smooth muscle cells. Recent researches showed that it had a protective function on blood vessels and could al... Background Cartilage oligomeric matrix protein (COMP) is mainly found in the skeletal system and vascular smooth muscle cells. Recent researches showed that it had a protective function on blood vessels and could also inhibit vascular calcification. We investigated the serum COMPs in coronary heart disease (CHD) patients, and the relationship between serum COMP and the calcification of coronary artery. Methods A total of 233 consecutive chest pain patients who first underwent coronary angiography followed by multi-slice computed to- mography (MSCT) within six months were recruited and divided into two groups according to the coronary angiography luminal diameter narrowing percentages: CHD group (diameter narrowing 〉 50%, n = 194) and control group (diameter narrowing 〈 50%, n = 39). The Gen- sini score, Syntax score and coronary artery calcium score (CACs) were calculated. The serum COMP level was determined using ELISA. Results The levels of COMP were significantly higher in the CHD group than in the control group 155.7 (124.5-194.5) ng/mL vs. 128.4 (113.0-159.9) ng/mL, P = 0.019. There were no correlation between COMP, Gensini score, Syntax score, severity of coronary stenosis and the number of coronary artery with stenosis 〉 50%. The serum COMP was correlated with age (r = 0.294, P 〈 0.001), fasting glucose (r = 0.163, P = 0.015), HbAlc (r = 0.194, P = 0.015) and CACs (r = 0.137, P = 0.037). Stepwise linear regression analysis showed that COMP level and age were independent predictors of CACs in the CHD patients (fl = 0.402, t = 2.612, P = 0.015; fl = 0.472, t = 3.077, P = 0.005). Performance of COMP for predicting CHD was shown as area under curve (AUC): 0.632, 95% CI: 0.549-0.715 and upper tertile CACs was AUC: 0.602, 95% CI: 0.5264).678 in receiver operating characteristic (ROC) curve analysis. Conclusion Calcification of coronary artery was an independent predictor of serum COMPs. 展开更多
关键词 Cartilage oligomeric matrix protein Coronary artery calcification Coronary heart disease
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Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells 被引量:1
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作者 Li Li Peng Gao +6 位作者 Hou-zao Chen Zhu-qin Zhang Ting-ting Xu Yu-yan Jia Hui-na Zhang Guan-hua Du De-pei Liu 《Chinese Medical Sciences Journal》 CAS CSCD 2013年第2期65-71,共7页
Objective To study the role of sirtuin 1 (SIRT1) in Fas ligand (FasL) expression regulation during vascular lesion formation and to elucidate the potential mechanisms. Methods SIRT1 and FasL protein levels were d... Objective To study the role of sirtuin 1 (SIRT1) in Fas ligand (FasL) expression regulation during vascular lesion formation and to elucidate the potential mechanisms. Methods SIRT1 and FasL protein levels were detected by Western blotting in either mouse arteries extract or the whole rat aortic vascular smooth muscle cell (VSMC) lysate. Smooth muscle cell (SMC)-specific human SIRT1 transgenic (Tg) C57BL/6 mice and their littermate wild-type (WT) controls underwent complete carotid artery ligation (ligation groups) or the ligation-excluded operation (sham groups). The carotid arteries were collected 1 day after operation. Reverse transcription-polymerase chain reaction was performed to detect the mRNA levels of SIRT1 and FasL. Luciferase reporter assays were performed to detect the effect of WT-SIRT1, a dominant-negative form of SIRT1 (SIRT1H363Y), and GATA-6 on the promoter activity of FasL. Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis. Results SIRTI was expressed in both rat aortic VSMCs and mouse arteries. Forced SIRT1 expression increased FasL expression both in injured mouse carotid arteries 1 day after ligation (P〈0.001) and VSMCs treated with serum (P〈0.05 at the transcriptional level, P〈0.001 at the protein level). No notable apoptosis was observed. Furthermore, transcription factor GATA-6 increased the promoter activity of FasL (P〈0.001). The induction of FasL promoter activity by GATA-6 was enhanced by WT-SIRT1 (P〈0.001), while SIRT1H363Y significantly relieved the enhancing effect of WT-SIRT1 on GATA-6 (P〈0.001). Conclusions Overexpression of SIRT1 up-regulates FasL expression in both flow-restricted mouse carotid arteries and serum-stimulated VSMCs. The transcription factor GATA-6 participates in the transcriptional regulation of FasL expression by SIRT 1. 展开更多
关键词 sirtuinl Fas ligand GATA-6 vascular smooth muscle cell
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Hepatic angiomyolipoma: Dynamic computed tomography features and clinical correlation 被引量:10
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作者 Bin Yang Wen-Hui Chen +2 位作者 Qiao-Yun Li Jing-Jing Xiang Ru-Jun Xu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第27期3417-3420,共4页
AIM: To study the dynamic computed tomography (CT) features of hepatic angiomyolipoma (AML) in patients with or without tuberous sclerosis complex (TSC).METHODS: The clinical information, CT fi ndings and histopatholo... AIM: To study the dynamic computed tomography (CT) features of hepatic angiomyolipoma (AML) in patients with or without tuberous sclerosis complex (TSC).METHODS: The clinical information, CT fi ndings and histopathological results of hepatic AML were analyzed retrospectively in 10 patients.RESULTS: Hepatic AML was prone to occur in female patients (7/10), and most of the patients (8/10) had no specific symptoms. All tumors presented as well-def ined, unenveloped nodules in the liver. Six patients with sporadic hepatic AML had a solitary hepatic nodule with a definite fat component. Non-fat components of the hepatic lesions were enhanced earlier and persistently. Prominent central vessels were noted in the portal venous phase in three patients. In four patients with hepatic AML and TSC, most of the nodules were within the peripheral liver. Seven fat-deficient nodules were found with earlier contrast enhancement and rapid contrast material washout in two patients. Lymphangioleiomyomatosis was found in one patient.CONCLUSION: Imaging features of hepatic AML are characteristic. Correct diagnosis preoperatively can be made in combination with clinical features. 展开更多
关键词 LYMPHANGIOLEIOMYOMATOSIS X-RAY Computed tomography ANGIOMYOLIPOMA Tuberous sclerosis complex
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Rosuvastatin inhibits the smooth muscle cell proliferation by targeting TNFα mediated Rho kinase pathway 被引量:3
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作者 Xiao Sun Hao Tong +1 位作者 Man Zharlg Xiao-Hang Wang 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2012年第2期180-184,共5页
Objective To investigate whether Tumor Necrosis Factor-alpha (TNFα) is capable of activating Rho kinase pathway which leads to smooth muscle cell proliferation and the intervention function of Rosuvastatin, and cla... Objective To investigate whether Tumor Necrosis Factor-alpha (TNFα) is capable of activating Rho kinase pathway which leads to smooth muscle cell proliferation and the intervention function of Rosuvastatin, and clarify the mechanism and intervention manner of anti-atherosclerosis by Rosuvastatin. Methods Wistar neonate rat smooth muscle cells were cultured, and the activity of cell proliferation was determined by methyl thiazolyl tetrazolium (MTT). The expression of Rho kinase genes after the stimulation of TNFα was evaluated by RT-PCR. Western blot method was used to measure the protein expression of proliferating cell nuclear antigen (PCNA) after TNFα stimulation and Rosuvastatin intervention in smooth muscle cell. Results The TNFα stimulation significantly enhanced the expression of Rho kinase and increased the expression of PCNA protein in smooth muscle cells (P 〈 0.05). These effects were positively correlated with prolonged treatment whereas additional Rosuvastatin administration inhibited the above-mentioned effects (P 〈 0.05). Conclusions The activation of TNFα mediated Rho kinase signaling pathway can significantly promote smooth muscle cell proliferation, and Rosuvastatin can not only inhibit this pathway but also the induced proliferation. 展开更多
关键词 Tumor necrosis factor-alpha Rho kinase Signaling transduction Vascular smooth muscle cell
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INTERLEUKIN 10 INHIBITS THE RAT VSMC PROLIFERATION AND COLLAGEN SECRETION STIMULATED BY ANGIOTENSIN II 被引量:3
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作者 夏春芳 霍勇 +2 位作者 尹航 朱国英 唐朝枢 《Chinese Medical Sciences Journal》 CAS CSCD 2001年第3期125-128,共4页
Objective. To study the effect of interleukin 10 (IL- 10) on the angiotensin II (AngII) stimulated rat VSMC proliferation and collagen secretion, and furthermore, explore its mechanism. Methods. On cultured VSMC of ra... Objective. To study the effect of interleukin 10 (IL- 10) on the angiotensin II (AngII) stimulated rat VSMC proliferation and collagen secretion, and furthermore, explore its mechanism. Methods. On cultured VSMC of rat, 3H- thymine (3H- TdR) and 3H- proline incorporations were used to evaluate the DNA and collagen synthesis, respectively. Western blot and immunoprecipitation were applied to assay the expression and activity of focal adhesion kinase (FAK), respectively. Results. IL- 10 (10- 8~ 10- 10g/ml) inhibited the increase of 3H- TdR and 3H- proline incorporation as well as FAK activity, which was induced by 10- 7mol/L AngII (P< 0.05 or P< 0.01). IL- 10 also obviously downregulated the synthesis and secretion of collagen by AngII stimulated VSMC. But there was no difference in the protein expression of FAK among all the groups (P >0.05). Conclusion. IL- 10 antagonizes the VSMC proliferation and collagen synthesis by regulating FAK activity stimulated by AngII. 展开更多
关键词 interleukin 10 angiotensin II focal adhesion kinase
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Baicalin inhibits PDGF-BB-stimulated vascular smooth muscle cell proliferation through suppressing PDGFRβ-ERK signaling and increase in p27 accumulation and prevents injury-induced neointimal hyperplasia 被引量:31
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作者 Li-Hua Dong Jin-Kun Wen +5 位作者 Sui-Bing Miao Zhenhua Jia Hai-Juan Hu Rong-Hua Sun Yiling Wu Mei Han 《Cell Research》 SCIE CAS CSCD 2010年第11期1252-1262,共11页
The increased proliferation and migration of vascular smooth muscle cells (VSMCs) are key events in the development of atherosclerotic lesions. Baicalin, an herb-derived flavonoid compound, has been previously shown... The increased proliferation and migration of vascular smooth muscle cells (VSMCs) are key events in the development of atherosclerotic lesions. Baicalin, an herb-derived flavonoid compound, has been previously shown to induce apoptosis and growth inhibition in cancer cells through multiple pathways. However, the potential role of baicalin in regulation of VSMC proliferation and prevention of cardiovascular diseases remains unexplored. In this study, we show that pretreatment with baicalin has a dose-dependent inhibitory effect on PDGF-BB-stimulated VSMC pro- liferation, accompanied with the reduction of proliferating cell nuclear antigen (PCNA) expression. We also show that baicalin-induced growth inhibition is associated with a decrease in cyclin E-CDK2 activation and increase in p27 level in PDGF-stimulated VSMCs, which appears to be at least partly mediated by blockade of PDGF recep- tor [~ (PDGFR~)-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling. In addition, baicalin was also found to inhibit adhesion molecule expression and cell migration induced by PDGF-BB in VSMCs. Furthermore, using an animal carotid arterial balloon-injury model, we found that baicalin significantly inhibited neointimal hyperplasia. Taken together, our results reveal a novel function of baicalin in inducing growth arrest of PDGF-stimulated VSMCs and suppressing neointimal hyperplasia after balloon injury, and suggest that the underlying mechanism involves the inhibition of cyclin E-CDK2 activation and the increase in p27 accumulation via blockade of the PDGFR^-ERK1/2 signaling cascade. 展开更多
关键词 BAICALIN vascular smooth muscle cells proliferation cyclin E neointimal hyperplasia
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Differentiation of CT scan diagnosis between minimal fat renal angiomyolipoma with sufficient blood supply and clear cell renal carcinoma 被引量:1
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作者 Yan Guo Qian Peng +3 位作者 Zhuo Wang Mingjuan Liu Xufeng Yang Teng Long 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第1期35-39,共5页
Objective: The aim of our study was to investigate the feature of minimal fat renal angiomyolipoma with sufficient blood supply using CT scans and improve the diagnosis accuracy required to differentiate it from clea... Objective: The aim of our study was to investigate the feature of minimal fat renal angiomyolipoma with sufficient blood supply using CT scans and improve the diagnosis accuracy required to differentiate it from clear cell renal carcinoma. Methods: Retrospective analysis of 24 cases of post-surgery confirmed angiomyolipoma with sufficient blood supply (total of 25 tumors) in our hospital that were used for a pathological comparison study. Results: Among the 24 patients diagnosed with angiomyolipoma, nobody had bloody urine, Of the 96 patients diagnosed with clear cell renal cancer, 14 had bloody urine (14.6%). In our studied group, the size of angiomyolipomas with sufficient blood supply was between 1.5 cm× 2.0 cm to 8.0 cm× 10.0 cm. During CT scan analysis, twenty tumors had similar density, and five of them had higher density. Only one tumor had a few dots of calcification (4%). Adipose tissue was not visible in 9 tumors, while 16 tumors had visible dots of adipose tissue, as visualized by CT scan. Intensive scanning indicated that all of the tumors showed a strong enhancement in the renal corticomedullary phase. Twenty tumors had significant heterogeneous enhancement in the early phase, while another set of five cases had homogenous prolonged enhancement. Nineteen patients had surgery to remove the angiomyolipomas, while six patients had single side kidney removal due to misdiagnosis for renal cancer in cases where the tumor severely compromised the renal parenchyma and sinus. All 25 cases were classified as renal angiomyolipoma by pathological analysis. Within the 96 cases of clear cell renal cancer, 64 tumors had relatively low density, 29 tumors had equal density, and 3 cases had relatively higher density. Fourteen of the tumors had calcification (14.6%), and none of them had visualized adipose tissue. Enhanced CT scans indicated that 69 cases of renal cancer showed significant enhancement in the renal corticomedu^ary phase, which had the abnormal pattern of "fast-in-and-fast-out". Additionally, 27 cases had slow and prolonged enhancement. Conclusion: Similar to clear cell renal carcinomas, angiomyolipomas with sufficient blood supply also appear to exhibit abnormal enhancement with a pattern of"fast-in-and-fast-out" during the early phase, which is easily misdiagnosed as renal cancer. It is difficult to differentiate them merely through CT scans; the key to differentiating them is to identify the adipose tissue within the tumor. Therefore, it is helpful to use thin-layer CT scans to locate the adipose tissue. 展开更多
关键词 renal tumor angiomyolipoma minimal fat clear cell renal carcinoma CT scan
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A case of hepatic angiomyolipoma difficult to distinguish from hepatocellular carcinoma 被引量:8
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作者 Masahiro Takahara Yasuhiro Miyake +6 位作者 Kazuyuki Matsumoto Daisuke Kawai Eisuke Kaji Tatsuya Toyokawa Morihito Nakatsu Masaharu Ando Mamoru Hirohata 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第23期2930-2932,共3页
We report a case of hepatic angiomyolipoma with uncommon clinical features. A 56-year-old man presented with a hepatic tumor in the caudate lobe. The tumor was hypoechoic on ultrasonography, showed early-phase hyperat... We report a case of hepatic angiomyolipoma with uncommon clinical features. A 56-year-old man presented with a hepatic tumor in the caudate lobe. The tumor was hypoechoic on ultrasonography, showed early-phase hyperattenuation on enhanced computed tomography and did not absorb iron on superparamagnetic iron oxide-enhanced magnetic resonance imaging. Hepatocellular carcinoma was highly suspected, and the patient underwent hepatic resection. Histologically, the tumor was mainly composed of smooth muscle cells and contained small amounts of adipose cells and blood vessels. On immunohistochemical staining, the smooth muscle cells were positive for a melanocytic cell-specific monoclonal antibody. In cases with uncommon features of angiomyolipoma, it is quite difficult to distinguish angiomyolipoma from hepatocellular carcinoma. 展开更多
关键词 Adipose cell Hepatic angiomyolipoma Hepatocellular carcinoma HMB-45 Smooth muscle cell
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C-TYPE NATRIURETIC PEPTIDE INHIBITS UPREGULATION OF α1-ADRENOCEPTOR AND INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN RAT VASCULAR SMOOTH MUSCLE AFTER VASCULAR ENDOTHELIAL INJURY
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作者 王晓红 杨军 +3 位作者 佟利家 苏静怡 唐朝枢 刘乃奎 《Chinese Medical Sciences Journal》 CAS CSCD 2000年第2期73-78,共6页
Objective.In a model of balloon injury of rat aortic endothelium, the effects of C-type natriuretic peptide(CNP) on α 1-adrenoreceptor and inositol 1,4,5-triphosphate (IP 3) receptor were st... Objective.In a model of balloon injury of rat aortic endothelium, the effects of C-type natriuretic peptide(CNP) on α 1-adrenoreceptor and inositol 1,4,5-triphosphate (IP 3) receptor were studied Methods. Aortic injuries were produced by vascular endothelium-denudation.α 1- adrenoreceptor in smooth muscle sarcolemma and IP 3 receptor in smooth muscle sarcoplasmic reticulum in the rat aorta were assayed by radioactive analysis method Results. It was found that neointima was formed and the contents of DNA, collagen and elastin of each intimamedia were significantly increased in 7 days and 21 days after balloon injury of rat aorta. α 1adrenoreceptor in smooth muscle sarcolemma and IP 3 receptor in sarcoplasmic reticulum were also upregulated. Results also showed that the administration of CNP ip significantly decreased the contents of DNA, collagen and elastin of each intimamedia, and inhibited the upregulation of α 1adrenoreceptor and IP 3 receptor Conclusion. The inhibition of the upregulation of α 1adrenoreceptor and IP 3 receptor by CNP might be one of the mechanisms of its suppressive action on intimal proliferation 展开更多
关键词 restenosis balloon angioplasty C-type natriuretic peptide
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