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野战综合手术方舱内微创介入救治闭合性肝损伤出血的护理模式探索 被引量:3
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作者 李菲 荣晶晶 +4 位作者 梁明 孙景阳 郭凯 那立夫 韩雅玲 《中国实用护理杂志》 2016年第22期-,共3页
目的:探讨自行研制的野战综合手术方舱,在战场一线或严重灾害现场等野外复杂环境下,对闭合性肝损伤出血实施紧急介入救治的护理配合模式。方法选取8只比格犬,利用比格犬构建肝损伤出血的大动物实验模型,在野战综合手术方舱内采用... 目的:探讨自行研制的野战综合手术方舱,在战场一线或严重灾害现场等野外复杂环境下,对闭合性肝损伤出血实施紧急介入救治的护理配合模式。方法选取8只比格犬,利用比格犬构建肝损伤出血的大动物实验模型,在野战综合手术方舱内采用微创介入的方式对损伤部位进行栓塞止血,并由护理人员按照不同于院内治疗的特殊护理模式进行术前、术中和术后的护理。结果8只肝损伤出血大动物模型均救治成功,且无手术相关并发症。结论在战场一线或严重灾害现场等野外复杂环境下,利用野战综合手术方舱实施介入救治,并采用不同于院内治疗的特殊护理模式,可有效救治闭合性肝损伤出血等重要脏器急重伤病。 展开更多
关键词 肝损伤出血 方舱 介入栓塞 护理
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Expression of Egr-1 in the liver of mice following hemorrhagic shock and resuscitation 被引量:1
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作者 梅建民 Timothy R.Billiar +2 位作者 于聪慧 David J.Gallo 杨荣华 《Journal of Medical Colleges of PLA(China)》 CAS 2005年第2期79-84,共6页
Objective: To investigate the expression of early growth response gene-1(Egr-1) in the liver following hemorrhagic shock with or without resuscitation(HS or HSR). Methods: Mice were subjected to HS or HSR.Liver expres... Objective: To investigate the expression of early growth response gene-1(Egr-1) in the liver following hemorrhagic shock with or without resuscitation(HS or HSR). Methods: Mice were subjected to HS or HSR.Liver expression of Egr-1 mRNA was detected by Northern blotting.DNA binding activity of the Egr-1 protein in liver nuclear extracts(NE) was determined by electrophoretic mobility shift assay(EMSA).Western blot analysis was used to assess the induction of Egr-1 protein in the liver tissue,cytoplasma and NE. Results: Egr-1 mRNA was strongly expressed as early as 1 h after HS,and its level was decreased in the following 2.5 h but still higher than that in 1 h HS group.The Egr-1 DNA binding activity elevated in the liver NE of 2.5 h HS group and 2.5 h HS + 4 h R group,so did the Egr-1 protein in the liver and the liver NE of the same two groups.However,the maximal Egr-1 protein expression was found in the cytoplasma of liver following 2.5 h HS. Conclusion: Our data suggest that both Egr-1 mRNA and protein are strongly elevated and the binding activity of Egr-1 to its cognate DNA site is increased in the liver following HSR,indicating the increases of Egr-1 transcriptional and translational levels.This study provides evidence that Egr-1 gene is activated in the liver during HS and HSR. 展开更多
关键词 early growth response gene-1(Egr-1) LIVERS hemorrhagic shock RESUSCITATION
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Role of transcription factor Egr-1 in liver injury following hemorrhagic shock and resuscitation
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作者 梅建民 Timothy R.Billiar +3 位作者 于聪慧 David J.Gallo 杨荣华 刘沙雷 《Journal of Medical Colleges of PLA(China)》 CAS 2005年第2期85-90,共6页
Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and ... Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and HSR injuries. The expressions of TNF-α, IL-6, G-CSF and ICAM-1 mRNAs in the liver were examined by RT-PCR, and their serum levels were measured by ELISA. The liver inflammatory infiltration and liver injury in both Egr-1 WT and KO mice following HS/R were evaluated by liver MPO content, serum ALT level and histological examination. Results: Egr-1 inhibition resulted in less mRNA expression of TNF-α, IL-6 , G-CSF and ICAM-1 in the liver, and lower serum levels of TNF-α, IL-6, G-CSF and ICAM-1 antigens in Egr-1 KO mice following HS/R. The liver inflammatory infiltration and liver injury were less severe in Egr-1 KO mice following HS/R, as evidenced by lower serum ALT level, lower hepatic MPO content and histological manifestations. Conclusion: Our data suggest that transcription factor Egr-1 is involved in regulating the expression of inflammatory response genes and plays a role in liver injury following HS/R. 展开更多
关键词 transcription factor Egr-1 LIVER inflammatory response hemorrhagic shock RESUSCITATION
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