品管圈活动在肝病病房护士针刺伤发生中的应用

目的:探讨品管圈(QCC)活动在肝病病房护士针刺伤发生中的应用效果。方法:成立QCC,按QCC活动的步骤实施活动,依据实施前后针刺伤发生数的变化确认活动效果。结果:实施QCC活动后肝病病房针刺伤发生数显著下降;目标达标率为122%,进步率为75%。结论:开展QCC活动能显著减少肝病病房针刺伤的发生次数,避免和减少护理人员感染疾病。

新型冠状病毒肺炎疫情下肝病病房“零感染”的防护策略

目的探讨新型冠状病毒肺炎形势下,作为定点收治医院的肝病病房有效防控新型冠状病毒肺炎,确保"零感染"的防护策略。方法通过对2020年1月20日至3月20日疫情期间科室采取的有关加强制度严格落实、进行各类人员针对性培训、严密的消毒隔离措施等的回顾性分析,进行肝病病房确保"零感染"的规范化护理管理策略的总结。结果 2020年1月20日至3月10日疫情期间我科共有医护人员31人,工勤人员2人,患者出院28人,新入24人,目前现有25人,医护人员、出入院患者、工勤人员实现"零感染"。结论全面有效的防控制度、工作流程,专业性的培训是避免医院内交叉感染发生及病毒传播的有效措施,为防止新型冠状病毒的院内交叉感染及其防御救治提供了重要保障。

Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease

AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds.

层级管理在肝病科优质护理服务中的应用

目的:分析探究层级管理在肝病科病房优质护理服务中的应用。方法:选取2015年6月~2017年5月到肝病科接受治疗肝病患者200例作为研究对象。按照就诊的时间不同分为对照组和观察组。2015年6月~2016年5月来肝病科就诊的90患者作为对照组,给予常规的护理服务。2016年6月~2017年5月就诊的110例患者作为观察组给予层级管理护理服务。观察两组患者护理前后患者的恢复情况及患者对两种管理方式的满意度。结果:在治疗前,两组护理评分无明显差异(P>0.05),观察组的评分明显高于对照组(P<0.05)。观察组满意度明显高于对照组(P<0.05)。结论:层级管理在肝病科病房优质护理服务中具有重要价值。