左归丸改善MSG-肝再生大鼠肝肾精血亏虚证的作用机制研究

To study the effect and the mechanism of Zuogui Pill on the deficiency of kidney essence and liver blood.Methods:Established the model of deficiency of kidney essence and liver blood of rat(the model of MSG regeneration rat).Observing the deficiency kidney essence and liver blood and ARN or regeneration liver of expression transforming growth factor type 1 beta(TGF β 1).Results:The liver regeneration of the model of MSG regeneration rat,postoperated on 11th day,the degree of liver regeneration was 0 58±0 03,the rate of liver weight/body weight was 3 27±0 12,MI was 0 11±0 02,RBC was 3 83±0 09×10 12 /L,Hb was 119 0±8 3g/L.The group of treatment by Zuogui Pill(dosage 5g/kg daily),postoperated on 11th day,the degree of liver regeneration was 0 84±0 04,the rate of liver weight/body weight was 3 85±0 14,MI was 0 11±0 02,RBC was 5 12±0 06×10 12 /L,Hb was 172 7±3 9g/L.The above datum were significantly different among the groups.The observation results showed MSG induced injury to ARN of the rat treated with monosodium glutamate.Comparing to the model of MSG regeneration rat group,it showed that Zuogui Pill could obviously inhibited the ARN injury in the rat treated with monosodium glutamate.The maladjustment in liver regeneration of the model of MSG regeneration rat,in the end,the liver regeneration was significantly inhibited.The effect of promoting liver regeneration in the model of MSG regeneration rat treated by gastrogavage Zuogui Pill.All data are reported as means±SE.When this analysis revealed a significant difference(p<0 05 or p<0 01).TGF β 1 has an obviously higher expression in liver and ARN during liver regeneration of the model of MSG regeneration rat.The effect of regulation of TGF β 1 expression in liver and ARN of the model of MSG regeneration rat treated by gastrogavage Zuogui Pill.The effect inhibited the deficiency of kidney essence and liver blood of the model MSG regeneration rat treated by gastrogavage Zuogui Pill.Conclusion:The deficiency of kidney essence and liver blood of the model of MSG regeneration rat can be improved gastrogavage Zuogui Pill.The effect of regulation TGF β 1 expression in liver and ARN of the model of MSG regeneration rat treated by gastrogavage.

“肝肾精血亏虚”大鼠动物模型的建立

目的 :研究“肝肾同源于精血”的病理生理机制、肝肾精血亏虚证本质及药物筛选 ,建立“肝肾精血亏虚”大鼠动物模型。方法 :于Wistar大鼠出生后第 2、4、6、8、10d皮下注射左旋谷氨酸单钠 (MSG)每次4mg g体重 ,第 8周手术切除MSG -大鼠大部分 (约 6 8% )肝脏。结果 :“肝肾精血亏虚大鼠”的肝再生度、肝细胞分裂指数 (% )、肝重 体重比值、骨髓有核细胞增生度、外周血红细胞计数和血红蛋白均显著低于正常和生理盐水对照组 (P <0 0 1)。经补益肝肾精血的左归丸治疗后 ,“肝肾精血亏虚大鼠”上述指标显著改善 (P<0 0 5,或P <0 0 1)。结论 :大部切除MSG 大鼠肝脏可制作“肝肾精血亏虚大鼠”动物模型 。