超声诊断肝脏吸虫病的临床研究

目的探讨超声诊断肝脏吸虫病临床诊断价值。方法分析120例肝脏吸虫病患者超声声像特点。结果诊断正确率为96.67%,有4例出现误诊。结论超声诊断肝吸虫病具有准确率高、无创、快捷的优势。

肝脏肺吸虫病的CT诊断

作者认为肺吸虫病发生在肝脏系少见病例，在ＣＴ认识和诊断上有待进一步提高，特别与肝结核、肝脏肿、肝包虫囊肿等鉴别诊断，仍存在一定的困难。临床上肺吸虫病多见脑部，肺，及皮下组织，我院病理科近十余年在肝脏发现肺吸虫病也是仅有一例，上述疾病ＣＴ表现均可出现低密度坏死区，不规则环状增强，斑点状钙化灶，单房及多房改变及大小不等的结节。组织学上的改变，肝细胞混浊肿胀，液化，变性，坏死，钙化，所不同处寄生虫病的病理组织学上可见夏科－雷登氏结晶，大量嗜酸性白细胞．本病与肝脓肿，肝结核的鉴别，在ＣＴ检查（包括动态ＣＴ）及肝动脉造影，Ｂ超检查，其它无更好方法鉴别。唯有病理检查证实，及职防部门专业鉴定。

常见食源性吸虫病及其诊治进展

食源性吸虫病是一类被忽视的热带病,据吸虫寄生的部位分为肝脏吸虫病、肺吸虫病及肠吸虫病。随着当前食物供应的全球化,食源性吸虫病呈现出感染人群数量增加和疾病分布区域面积增大的趋势。本文就常见食源性吸虫病及其诊治进展进行概述。

Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica

To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). Suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully, the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.