Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. Th...Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. The rats were randomly divided in to 4 groups （2 experimental groups; group AI and A2, and 2 control groups;group Bland B2 each containing 16 rats）. The experimental groups were given a single dose of doxorubicin i.e. 10 mg/kg body weight intraperitoneally and sacrificed after 7 days and 14 days for each group. Rats under control groups were given a single intraperitoneal dose of 2.5 ml/kg body weight normal saline and sacrificed with their respective experimental groups. A significant difference （p 〈 0.001） and （p = 0.005） in final body weight was observed among group A1 experimental and BI control rats, and among group A2 experimental and B2 control rats. As compared to group B2 control, group A2 experimental rats had significantly （p = 0.043） lower liver weight. Diameter ofhepatocyte （p 〈 0.001 for both groups） and nucleus （p=0.004 for group A1 and control BI, and p 〈 0.001 for group A2 and control B2） was significantly higher in experimental rats as compared to their respective controls. Cross-sections of the liver of both control groups had normal architecture. However, there was progressive destruction of liver architecture across the experimental groups. Doxorubicin causes the disruption of normal architecture of liver in rats. Thus care needs to be taken during doxorubicin chemotherapy to minimize effects on river.展开更多
为初步探究青钱柳多糖(CPP)对高脂血症小鼠降血脂作用和机制,将高脂血症小鼠随机分为模型对照组、辛伐他汀组、空白对照组以及青钱柳低、中、高剂量组,每组16组。模型对照组与空白对照组均喂以等量的无菌水,辛伐他汀组喂以4 mg/kg的辛...为初步探究青钱柳多糖(CPP)对高脂血症小鼠降血脂作用和机制,将高脂血症小鼠随机分为模型对照组、辛伐他汀组、空白对照组以及青钱柳低、中、高剂量组,每组16组。模型对照组与空白对照组均喂以等量的无菌水,辛伐他汀组喂以4 mg/kg的辛伐他汀与CMC-Na(0.5%)的混悬液,青钱柳低、中、高剂量组分别喂以100 mg/kg、200 mg/kg和400 mg/kg的CPP水溶液,连续喂养4周。实验结束后,测定血清TC、TG、HDL-C、LDL-C、FFA水平和LPS活力,取肝脏做病理形态学观察,用RT-PCR方法测定HSL m RNA的表达量。结果表明,与模型对照组相比,CPP组小鼠血清TC、TG、LDL-C的水平和LPS的活力(P<0.01)均显著降低,HDL-C和FFA的水平(P<0.01)则显著增加;CPP组小鼠肝脂变程度较模型组有不同程度的改善;相比于模型对照组,CPP组的HSL m RNA的表达量均显著上升(P<0.05)。综上,CPP可以明显降低高脂血症小鼠血脂水平,改善其因摄入过多脂质而导致肝脏脂肪变性。展开更多
Objective: To investigate the histopathological changes in the liver and other organs after impact injury. Methods: The rabbits were impacted with a BIM IV biological impacting machine at the xiphoid process. The seve...Objective: To investigate the histopathological changes in the liver and other organs after impact injury. Methods: The rabbits were impacted with a BIM IV biological impacting machine at the xiphoid process. The severity of liver injury was graded and scored through gross anatomy. At the same time, the pathological changes in the liver, heart, and lung were observed by light and electron microscopes. Results: Light microscopy showed that the pathological changes in the liver were: 1) loss of normal structure, hemorrhage and distortion of hepatic lobules; 2) cloudy swelling, degeneration, vacuolation and necrosis of liver cells; 3) infiltration of neutrophils. The lungs were injured and there were liver cell emboli in the small pulmonary arteries. Electron microscopy showed that the ultrastructure of the liver cells was severely damaged and the cells had significant features of necrosis. Conclusions: The major pathomorphological changes in the liver after impact injury are hemorrhage and necrosis. They may be complicated by exfoliation of liver cells to hepatic sinusoids. These cells circulate with the blood to form emboli in the pulmonary blood vessels.展开更多
文摘Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. The rats were randomly divided in to 4 groups （2 experimental groups; group AI and A2, and 2 control groups;group Bland B2 each containing 16 rats）. The experimental groups were given a single dose of doxorubicin i.e. 10 mg/kg body weight intraperitoneally and sacrificed after 7 days and 14 days for each group. Rats under control groups were given a single intraperitoneal dose of 2.5 ml/kg body weight normal saline and sacrificed with their respective experimental groups. A significant difference （p 〈 0.001） and （p = 0.005） in final body weight was observed among group A1 experimental and BI control rats, and among group A2 experimental and B2 control rats. As compared to group B2 control, group A2 experimental rats had significantly （p = 0.043） lower liver weight. Diameter ofhepatocyte （p 〈 0.001 for both groups） and nucleus （p=0.004 for group A1 and control BI, and p 〈 0.001 for group A2 and control B2） was significantly higher in experimental rats as compared to their respective controls. Cross-sections of the liver of both control groups had normal architecture. However, there was progressive destruction of liver architecture across the experimental groups. Doxorubicin causes the disruption of normal architecture of liver in rats. Thus care needs to be taken during doxorubicin chemotherapy to minimize effects on river.
文摘为初步探究青钱柳多糖(CPP)对高脂血症小鼠降血脂作用和机制,将高脂血症小鼠随机分为模型对照组、辛伐他汀组、空白对照组以及青钱柳低、中、高剂量组,每组16组。模型对照组与空白对照组均喂以等量的无菌水,辛伐他汀组喂以4 mg/kg的辛伐他汀与CMC-Na(0.5%)的混悬液,青钱柳低、中、高剂量组分别喂以100 mg/kg、200 mg/kg和400 mg/kg的CPP水溶液,连续喂养4周。实验结束后,测定血清TC、TG、HDL-C、LDL-C、FFA水平和LPS活力,取肝脏做病理形态学观察,用RT-PCR方法测定HSL m RNA的表达量。结果表明,与模型对照组相比,CPP组小鼠血清TC、TG、LDL-C的水平和LPS的活力(P<0.01)均显著降低,HDL-C和FFA的水平(P<0.01)则显著增加;CPP组小鼠肝脂变程度较模型组有不同程度的改善;相比于模型对照组,CPP组的HSL m RNA的表达量均显著上升(P<0.05)。综上,CPP可以明显降低高脂血症小鼠血脂水平,改善其因摄入过多脂质而导致肝脏脂肪变性。
文摘Objective: To investigate the histopathological changes in the liver and other organs after impact injury. Methods: The rabbits were impacted with a BIM IV biological impacting machine at the xiphoid process. The severity of liver injury was graded and scored through gross anatomy. At the same time, the pathological changes in the liver, heart, and lung were observed by light and electron microscopes. Results: Light microscopy showed that the pathological changes in the liver were: 1) loss of normal structure, hemorrhage and distortion of hepatic lobules; 2) cloudy swelling, degeneration, vacuolation and necrosis of liver cells; 3) infiltration of neutrophils. The lungs were injured and there were liver cell emboli in the small pulmonary arteries. Electron microscopy showed that the ultrastructure of the liver cells was severely damaged and the cells had significant features of necrosis. Conclusions: The major pathomorphological changes in the liver after impact injury are hemorrhage and necrosis. They may be complicated by exfoliation of liver cells to hepatic sinusoids. These cells circulate with the blood to form emboli in the pulmonary blood vessels.
