期刊文献+
共找到8篇文章
< 1 >
每页显示 20 50 100
HPLC测定肝达片中熊果酸的含量 被引量:4
1
作者 裴建梅 颜杰 贾孟良 《华西药学杂志》 CAS CSCD 北大核心 2005年第4期365-366,共2页
目的建立测定肝达片中熊果酸含量的HPLC方法.方法采用ODS柱,流动相为甲醇-水-磷酸(86∶14∶0.1),流速1.0 ml·min-1,检测波长208 nm,进样量20 μl.结果熊果酸在0.4~4.0 μg范围内呈良好的线性关系(r=0.9996),平均回收率为101.7%,RS... 目的建立测定肝达片中熊果酸含量的HPLC方法.方法采用ODS柱,流动相为甲醇-水-磷酸(86∶14∶0.1),流速1.0 ml·min-1,检测波长208 nm,进样量20 μl.结果熊果酸在0.4~4.0 μg范围内呈良好的线性关系(r=0.9996),平均回收率为101.7%,RSD=1.24%.结论所用方法准确、可靠、简便,精密度好,可对肝达片进行质量控制. 展开更多
关键词 肝达片 熊果酸 高效液相色谱
下载PDF
肝达片联合治疗慢性乙型肝炎疗效观察 被引量:2
2
作者 李光 刘舜贤 +1 位作者 余拉结 汤如卿 《中国现代医生》 2008年第20期73-74,共2页
目的观察肝达片、抗乙肝免疫核糖核酸、潘生丁联合应用对慢性乙型肝炎的疗效。方法治疗组78例,使用肝达片、抗乙肝免疫核糖核酸、潘生丁和一般保肝药物;对照组68例,仅使用一般护肝药物,3个月为1疗程。结果两组综合疗效及乙肝病毒复制指... 目的观察肝达片、抗乙肝免疫核糖核酸、潘生丁联合应用对慢性乙型肝炎的疗效。方法治疗组78例,使用肝达片、抗乙肝免疫核糖核酸、潘生丁和一般保肝药物;对照组68例,仅使用一般护肝药物,3个月为1疗程。结果两组综合疗效及乙肝病毒复制指标HBeAg、HBV-DNA相比较,差异有显著性。结论肝达片联合抗乙肝免疫核糖核酸、潘生丁治疗慢性乙型肝炎疗效较好,副作用少。 展开更多
关键词 肝达片 抗乙免疫核糖核酸 潘生丁 慢性乙型
下载PDF
肝达康片中茜草的薄层色谱鉴别方法探讨 被引量:3
3
作者 欧阳荣 胡铁骊 《中国中医药信息杂志》 CAS CSCD 2001年第10期38-38,共1页
关键词 茜草 薄层色谱法 鉴别
下载PDF
肝达康片治疗肝郁脾虚证非酒精性脂肪肝的临床观察 被引量:3
4
作者 张玉喜 马晓瑞 +2 位作者 黄雪梅 李阳 王海燕 《宁夏医学杂志》 CAS 2016年第10期916-918,共3页
目的探讨治疗非酒精性脂肪肝(NAFLD)肝郁脾虚证的方法。方法将120例NAFLD患者随机分为治疗组60例和对照组60例。治疗组给予肝达康片口服,对照组给予西药易善复(多烯磷脂酰胆碱)胶囊口服,连续服用3个月,观察2组患者治疗前后NAFLD的改善... 目的探讨治疗非酒精性脂肪肝(NAFLD)肝郁脾虚证的方法。方法将120例NAFLD患者随机分为治疗组60例和对照组60例。治疗组给予肝达康片口服,对照组给予西药易善复(多烯磷脂酰胆碱)胶囊口服,连续服用3个月,观察2组患者治疗前后NAFLD的改善情况。结果 2组患者治疗后肝/脾CT比值均明显改善(P<0.05),且治疗组在改善肝/脾CT比值方面优于对照组(P<0.05)。治疗组在降低TC、γ-GT方面有明显作用(P<0.05),在降低LDL-C、TG、ALT、AST方面作用尤为显著(P<0.05);对照组在降低TG、TC、AST、γ-GT方面有明显作用(P<0.05),在降低ALT方面作用尤为显著(P<0.05),治疗组在降低LDL-C方面优于对照组(P<0.05)。治疗组在肝区不适、乏力方面有明显改善(P<0.05),在食欲减退、舌淡或有瘀点、脉弦或涩方面改善尤为显著(P<0.05);对照组在乏力、食欲减退方面有明显改善(P<0.05),治疗组在食欲减退、舌淡或有瘀点、脉弦或涩方面改善优于对照组(P<0.05)。治疗组总有疗效为95.0%,对照组总有效为80%,治疗组优于对照组(P<0.05)。结论口服肝达康片能改善NAFLD患者的肝/脾CT比值以及血清γ-GT、ALT、AST、TC、TG、LDLC等生化指标,也可改善患者肝区不适、食欲减退、乏力、舌淡或有瘀点、脉弦或涩等症状、体征,提高临床总体疗效,是治疗NAFLD肝郁脾虚证的有效药物。 展开更多
关键词 非酒精性脂肪 临床疗效
下载PDF
肝达康片联合阿德福韦酯治疗慢性乙型肝炎的临床研究 被引量:8
5
作者 王文华 彭期兵 +1 位作者 夏平 李航 《现代药物与临床》 CAS 2019年第9期2756-2760,共5页
目的探讨肝达康片联合阿德福韦酯胶囊治疗慢性乙型肝炎临床疗效。方法选取2017年5月—2018年5月在重庆市大足区人民医院治疗的慢性乙型肝炎患者96例,根据入院号分为对照组(48例)和治疗组(48例)。对照组口服阿德福韦酯胶囊,10 mg/次,1次... 目的探讨肝达康片联合阿德福韦酯胶囊治疗慢性乙型肝炎临床疗效。方法选取2017年5月—2018年5月在重庆市大足区人民医院治疗的慢性乙型肝炎患者96例,根据入院号分为对照组(48例)和治疗组(48例)。对照组口服阿德福韦酯胶囊,10 mg/次,1次/d;治疗组在对照组基础上口服肝达康片,3 g/次,3次/d。入组者均治疗12周。观察两组患者临床疗效,同时比较治疗前后两组患者HBV-DNA和HBe Ag转阴率、肝功能、肝纤维化、血清学指标及CLDQ和SF-36量表评分。结果治疗后,对照组和治疗组临床有效率分别为81.25%和97.92%,两组比较差异具有统计学意义(P<0.05)。治疗后,治疗组患者HBV-DNA、HBe Ag转阴率明显高于对照组(P<0.05)。治疗后,两组丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、总胆汁酸(TBA)、透明质酸(HA)、层粘连蛋白(LN)、四型胶原(Ⅳ-C)、Ⅲ型前胶原N端肽(PC-Ⅲ)、白细胞介素-17(IL-17)、巨噬细胞移动抑制因子(MIF)、肿瘤坏死因子-α(TNF-α)、白细胞介素-22(IL-22)、转化生长因子-β1(TGF-β1)水平显著降低(P<0.05),且治疗组患者这些指标明显低于对照组(P<0.05)。治疗后,两组CLDQ量表评分、SF-36量表评分均显著升高(P<0.05),且治疗组患者CLDQ和SF-36量表评分明显高于对照组(P<0.05)。结论肝达康片联合阿德福韦酯胶囊治疗慢性乙型肝炎可有效改善患者肝功能,降低机体炎症反应,促进HBV-DNA和HBeAg转阴,利于患者生活质量提高。 展开更多
关键词 阿德福韦酯胶囊 慢性乙型 纤维化 总胆红素 层粘连蛋白 巨噬细胞移动抑制因子
原文传递
Wnt5a participates in hepatic stellate cell activation observed by gene expression profile and functional assays 被引量:18
6
作者 Wu-Jun Xiong Li-Juan Hu +4 位作者 Yi-Cheng Jian Li-Jing Wang Ming Jiang Wei Li Yi He 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第15期1745-1752,共8页
AIM:To identify differentially expressed genes in quiescent and activated hepatic stellate cells(HSCs)and explore their functions.METHODS:HSCs were isolated from the normal Sprague Dawley rats by in suit perfusion of ... AIM:To identify differentially expressed genes in quiescent and activated hepatic stellate cells(HSCs)and explore their functions.METHODS:HSCs were isolated from the normal Sprague Dawley rats by in suit perfusion of collagenase and pronase and density Nycodenz gradient centrifugation.Total RNA and mRNA of quiescent HSCs,and cultureactivated HSCs were extracted,quantified and reversely transcripted into cDNA.The global gene expression profile was analyzed by microarray with Affymetrix rat genechip.Differentially expressed genes were annotated with Gene Ontology(GO)and analyzed with Kyoto encyclopedia of genes and genomes(KEGG)pathway using the Database for Annotation,Visualization and Integrated Discovery.Microarray data were validated by quantitative real-time polymerase chain reaction(qRTPCR).The function of Wnt5a on human HSCs line LX-2 was assessed with lentivirus-mediated Wnt5a RNAi.The expression of Wnt5a in fibrotic liver of a carbon tetrachloride(CCl4)-induced fibrosis rat model was also analyzed with Western blotting.RESULTS:Of the 28 700 genes represented on this chip,2566 genes displayed at least a 2-fold increase or decrease in expression at a P<0.01 level with a false discovery rate.Of these,1396 genes were upregulated,while 1170 genes were downregulated in culture-activated HSCs.These differentially expressed transcripts were grouped into 545 GO based on biological process GO terms.The most enriched GO terms included response to wounding,wound healing,regulation of cell growth,vasculature development and actin cytoskeleton organization.KEGG pathway analysis revealed that Wnt5a signaling pathway participated in the activation of HSCs.Wnt5a was significantly increased in cultureactivated HSCs as compared with quiescent HSCs.qRTPCR validated the microarray data.Lentivirus-mediated suppression of Wnt5a expression in activated LX-2 resulted in significantly impaired proliferation,downregulated expressions of typeⅠcollagen and transforming growth factor-β1.Wnt5a was upregulated in the fibrotic liver of a CCl4-induced fibrosis rat model.CONCLUSION:Wnt5a is involved in the activation of HSCs,and it may serve as a novel therapeutic target in the treatment of liver fibrosis. 展开更多
关键词 Hepatic stellate cells WNT5A MICROARRAY Bioinformatics analyses Liver fibrosis
下载PDF
肝功能正常的乙肝患者要不要治疗
7
作者 张斌 《家庭用药》 2005年第10期15-15,共1页
我今年33岁,男性,被检查出乙肝“大三阳”已有四年了,肝功能检查一直正常。起初医生说肝功能正常不需要治疗,治也治不好。后来身体感觉更差,浑身无力,脸色发黄,食欲差。又看过几个医生,吃了几种药,仍未见好转。前几天做了个检... 我今年33岁,男性,被检查出乙肝“大三阳”已有四年了,肝功能检查一直正常。起初医生说肝功能正常不需要治疗,治也治不好。后来身体感觉更差,浑身无力,脸色发黄,食欲差。又看过几个医生,吃了几种药,仍未见好转。前几天做了个检查,肝功能正常,但DNA测出乙肝病毒含量高达10×10^8,医生仍说不用治疗,在我自己的要求下才给开了一些药吃。请问我的病怎么治? 展开更多
关键词 功能 乙型炎病毒 肝达片 清热解毒冲剂
原文传递
Gene Expression Profiles in Porcine Tissues of Liver and Kidney
8
作者 Tatiana Glazko Nataliya Khlopova +2 位作者 Scott Fahrenkrug John Garbe Valeriy Glazko 《Journal of Life Sciences》 2011年第3期192-200,共9页
Microarray technologies are widely used all over the world for the gene expression analysis in various tissues, but still this technology has some limitations. The problem of eliminated reproducibility of the results,... Microarray technologies are widely used all over the world for the gene expression analysis in various tissues, but still this technology has some limitations. The problem of eliminated reproducibility of the results, obtained in different laboratories using different platforms, is very relevant nowadays. For revelation of problems ofmicroarrays, the comparative analysis of hepatic and renal gene expression profiles (GEP) was carried out by using swine protein annotated oligonucleotide microarrays (SPAM). Revealed differences in GEP between kidney and liver of pigs were correlated with functional and histological distinctions of these organs. It was shown that sources of errors in the comparative analysis of organ-specific GEP could be connected to the cross hybridization of one probe to transcripts (cDNA of mRNA) of different genes and to individual variability in gene expression between animals, related with the changeability of influences of exo- and endogenous regulation factors. 展开更多
关键词 Oligonucleotide microarrays gene expression cross hybridization individual variability.
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部